2001
High-Yield Synthesis and Purification of an α-Helical Transmembrane Domain
Fisher L, Engelman D. High-Yield Synthesis and Purification of an α-Helical Transmembrane Domain. Analytical Biochemistry 2001, 293: 102-108. PMID: 11373085, DOI: 10.1006/abio.2001.5122.Peer-Reviewed Original ResearchAmino AcidsChromatography, High Pressure LiquidGlycophorinsPeptidesProtein Structure, SecondarySpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
1985
Stability of transmembrane regions in bacteriorhodopsin studied by progressive proteolysis
Dumont M, Trewhella J, Engelman D, Richards F. Stability of transmembrane regions in bacteriorhodopsin studied by progressive proteolysis. The Journal Of Membrane Biology 1985, 88: 233-247. PMID: 3913776, DOI: 10.1007/bf01871088.Peer-Reviewed Original ResearchConceptsMolecular weight distributionFragments of bacteriorhodopsinVisible absorption spectraX-ray diffractionX-ray diffraction patternsDiffraction patternsAqueous mediaNative purple membraneUrea-polyacrylamide gel electrophoresisWeight distributionSmall soluble peptidesAbsorption spectraHydrophobic segmentsBacteriorhodopsin sequenceAmino acid analysisHigh-pressure liquid chromotographyPolyacrylamide gel electrophoresisDigestion conditionsPurple membraneOptical absorptionSoluble peptidesBacteriorhodopsinMembrane-embedded regionsLiquid chromotographyProducts of digestion