2000
Transfer RNA Identity Change in Anticodon Variants of E. coli tRNAPhe in Vivo
Kim H, Kim I, Söll D, Lee Y. Transfer RNA Identity Change in Anticodon Variants of E. coli tRNAPhe in Vivo. Molecules And Cells 2000, 10: 76-82. PMID: 10774751, DOI: 10.1007/s10059-000-0076-7.Peer-Reviewed Original ResearchConceptsMutant tRNA genesMutant tRNAsTRNA genesAnticodon sequenceAnticodon mutantsHost viabilityE. coliAmino acidsMost aminoacyl-tRNA synthetasesOpal stop codonAminoacyl-tRNA synthetasesSite-directed mutagenesisE. coli tRNAMajor recognition elementAnticodon variantsSuch tRNAsTRNAStop codonAminoacylation specificityAnticodonSimilarity dendrogramVivo evolutionGenesAcceptor specificityAnticodon change
1994
Coexpression of eukaryotic tRNASer and yeast seryl-tRNA synthetase leads to functional amber suppression in Escherichia coli
Weygand-Durasević I, Nalaskowska M, Söll D. Coexpression of eukaryotic tRNASer and yeast seryl-tRNA synthetase leads to functional amber suppression in Escherichia coli. Journal Of Bacteriology 1994, 176: 232-239. PMID: 8282701, PMCID: PMC205035, DOI: 10.1128/jb.176.1.232-239.1994.Peer-Reviewed Original ResearchConceptsSeryl-tRNA synthetaseYeast seryl-tRNA synthetaseEscherichia coliSerine tRNA geneE. coliConservation of determinantsTRNA genesSchizosaccharomyces pombePrimary transcriptPlasmid promoterAmber suppressionTRNA identityFunctional expressionColiCoexpressionSynthetasePombeGenesPromoterSuppressorTranscriptsOrganismsConservationExpressionEfficient suppression
1988
Site-directed mutagenesis to fine-tune enzyme specificity
Uemura H, Rogers M, Swanson R, Watson L, Söll D. Site-directed mutagenesis to fine-tune enzyme specificity. Protein Engineering Design And Selection 1988, 2: 293-296. PMID: 3150543, DOI: 10.1093/protein/2.4.293.Peer-Reviewed Original ResearchConceptsOligonucleotide-directed mutagenesisEscherichia coli glutaminyl-tRNA synthetaseGenetic selectionGlutaminyl-tRNA synthetaseAmino acid replacementsSite-directed mutagenesisAcid replacementsEnzyme specificitySingle residueMutagenesisSide chainsRepulsive charge-charge interactionsSpecific recognitionCharge-charge interactionsNucleic acidsMutantsProteinSupFSynthetaseResiduesGlutamineSelectionThe 5′-terminal guanylate of chloroplast histidine tRNA is encoded in its gene.
Burkard U, Söll D. The 5′-terminal guanylate of chloroplast histidine tRNA is encoded in its gene. Journal Of Biological Chemistry 1988, 263: 9578-9581. PMID: 2838471, DOI: 10.1016/s0021-9258(19)81555-7.Peer-Reviewed Original ResearchAnimalsBase SequenceChloroplastsCyanobacteriaDNA, RecombinantEndoribonucleasesEscherichia coliEscherichia coli ProteinsEuglena gracilisGuanine NucleotidesGuanosine MonophosphateMolecular Sequence DataPentosyltransferasesPlantsRibonuclease PRNA PrecursorsRNA, Transfer, Amino Acid-SpecificRNA, Transfer, HisSaccharomyces cerevisiaeVegetables
1983
Each element of the Drosophila tRNA Arg gene split promoter directs transcription in Xenopus oocytes
Sharp S, Dingermann T, Schaack J, Sharp J, Burke D, DeRobertis E, Söll D. Each element of the Drosophila tRNA Arg gene split promoter directs transcription in Xenopus oocytes. Nucleic Acids Research 1983, 11: 8677-8690. PMID: 6561520, PMCID: PMC326616, DOI: 10.1093/nar/11.24.8677.Peer-Reviewed Original ResearchConceptsD-control regionDrosophila tRNAArg geneTRNAArg geneEukaryotic tRNA gene transcriptionXenopus oocytesRNA polymerase III transcription factorT-control regionEukaryotic tRNA genesTRNA gene transcriptionIntragenic control regionTranscription initiation siteSpecific DNA sequencesTRNA genesTranscription initiationArg genesControl regionTranscription factorsGene transcriptionDNA sequencesMutant formsOocyte nucleusSequence 5Initiation siteGenesTranscription
1981
Internal control regions for transcription of eukaryotic tRNA genes.
Sharp S, DeFranco D, Dingermann T, Farrell P, Söll D. Internal control regions for transcription of eukaryotic tRNA genes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1981, 78: 6657-6661. PMID: 6947245, PMCID: PMC349108, DOI: 10.1073/pnas.78.11.6657.Peer-Reviewed Original ResearchThe initiator tRNA genes of Drosophila melanogaster: evidence for a tRNA pseudogene
Sharp S, DeFranco D, Silberklang M, Hosbach H, Schmidt T, Kubli E, Gergen J, Wensink P, Söll D. The initiator tRNA genes of Drosophila melanogaster: evidence for a tRNA pseudogene. Nucleic Acids Research 1981, 9: 5867-5882. PMID: 6273811, PMCID: PMC327570, DOI: 10.1093/nar/9.22.5867.Peer-Reviewed Original ResearchConceptsInitiator tRNA geneTRNA genesDrosophila genomeDrosophila melanogasterTRNA gene familyRNA polymerase IIIDrosophila melanogaster DNAKb DNA fragmentTRNA pseudogenesRepetitive DNAGene familyPolymerase IIISingle copyGenomic DNADNA fragmentsPseudogenesRepeat segmentsGenesHybridization analysisFourth cloneMelanogasterSitu hybridizationGenomeDNAFragments
1980
Two control regions for eukaryotic tRNA gene transcription.
DeFranco D, Schmidt O, Söll D. Two control regions for eukaryotic tRNA gene transcription. Proceedings Of The National Academy Of Sciences Of The United States Of America 1980, 77: 3365-3368. PMID: 6774336, PMCID: PMC349616, DOI: 10.1073/pnas.77.6.3365.Peer-Reviewed Original ResearchConceptsControl regionTRNALys geneGene transcriptionEukaryotic tRNA gene transcriptionTRNA gene transcriptionInternal control regionIdentical coding sequenceShort leader sequencePrecursor tRNAsTranscription initiationMature tRNALeader sequenceCoding sequencePBR322 sequencesNuclear extractsRecombinant plasmidTranscriptionXenopus oocytesGenesTRNASequenceAnalysis of a drosophila tRNA gene cluster
Hovemann B, Sharp S, Yamada H, Söll D. Analysis of a drosophila tRNA gene cluster. Cell 1980, 19: 889-895. PMID: 6769590, DOI: 10.1016/0092-8674(80)90080-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceComputersDNA, RecombinantDrosophila melanogasterGenesGenetic LinkagePlasmidsRNA, TransferTranscription, GeneticConceptsTRNA genesFlanking regionsTRNA gene clusterDrosophila tRNA genesTranscription control regionsSecondary structure featuresTranscription directionDrosophila DNAGene clusterMature tRNATRNALys geneControl regionDNA sequencesNucleotide sequenceConsensus sequenceGene regionKb fragmentTerminal sequenceGenesCol E1DNA strandsDNATRNAHybridization of DNASequence
1979
The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts.
Silverman S, Schmidt O, Söll D, Hovemann B. The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts. Journal Of Biological Chemistry 1979, 254: 10290-10294. PMID: 114522, DOI: 10.1016/s0021-9258(19)86707-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceDNA, RecombinantDrosophila melanogasterFemaleOvumRibonucleasesRNA, TransferTranscription, GeneticXenopusConceptsMature tRNAPrecursor RNANucleotide sequenceDrosophila tRNAArg geneGerminal vesicle extractsArginine tRNA geneXenopus germinal vesicleTRNA genesTRNAArg genePrimary transcriptDNA sequencesLeader sequenceVesicle extractsT residuesGenesTranscriptionGerminal vesicleTerminusVitro systemTRNAA sequenceRNASequenceNucleotidesTRNAArgAminoacyl-tRNA Synthetases: General Features and Recognition of Transfer RNAs
Schimmel P, Söll D. Aminoacyl-tRNA Synthetases: General Features and Recognition of Transfer RNAs. Annual Review Of Biochemistry 1979, 48: 601-648. PMID: 382994, DOI: 10.1146/annurev.bi.48.070179.003125.Peer-Reviewed Original Research
1978
Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts.
Schmidt O, Mao J, Silverman S, Hovemann B, Söll D. Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts. Proceedings Of The National Academy Of Sciences Of The United States Of America 1978, 75: 4819-4823. PMID: 105357, PMCID: PMC336212, DOI: 10.1073/pnas.75.10.4819.Peer-Reviewed Original ResearchConceptsGerminal vesicle extractsTRNA genesRNA speciesVesicle extractsEukaryotic gene transcriptionEukaryotic tRNA genesRegulatory DNA regionsPrecursor tRNA moleculesXenopus germinal vesiclePrecursor tRNAsSpecific transcriptionDNA regionsTRNA biosynthesisTRNA precursorsNucleotide modificationsGene transcriptionTRNA moleculesTranscription systemNucleotide analysisTRNAGerminal vesicleGenesTranscriptionTRNALysSpecies