1990
Purification and characterization of Chlamydomonas reinhardtii chloroplast glutamyl-tRNA synthetase, a natural misacylating enzyme.
Chen M, Jahn D, Schön A, O'Neill G, Söll D. Purification and characterization of Chlamydomonas reinhardtii chloroplast glutamyl-tRNA synthetase, a natural misacylating enzyme. Journal Of Biological Chemistry 1990, 265: 4054-4057. PMID: 2303494, DOI: 10.1016/s0021-9258(19)39701-7.Peer-Reviewed Original ResearchConceptsGlutamyl-tRNA synthetaseChloroplast enzymeApparent molecular massSequential column chromatographyChlamydomonas reinhardtiiActive enzymeMolecular massNondenaturing conditionsEscherichia coliDenaturing conditionsAcceptor RNASynthetaseMono S.Mono QEnzymeTRNAReinhardtiiYeastColumn chromatographyRNACytoplasmicProteinBarleyColiReversed phase chromatography
1983
Partial purification of Drosophila Kc cell RNA polymerase III transcription components. Evidence for shared 5 S RNA and tRNA gene factors.
Burke D, Schaack J, Sharp S, Söll D. Partial purification of Drosophila Kc cell RNA polymerase III transcription components. Evidence for shared 5 S RNA and tRNA gene factors. Journal Of Biological Chemistry 1983, 258: 15224-15231. PMID: 6197413, DOI: 10.1016/s0021-9258(17)43797-5.Peer-Reviewed Original ResearchConceptsS RNA geneTranscription componentsRNA polymerase IIIRNA genesPolymerase IIIStable transcription complex formationReconstitution of transcriptionTranscription complex formationDrosophila Kc cellsCM-Sepharose column chromatographyDrosophila tRNAKc cellsTranscription factorsS RNATRNAGene factorsCompetition experimentsTranscriptionGenesComplex formationPartial purificationDEAE-SephadexReconstitutionCofractionationColumn chromatography
1981
Partial purification of RNase P from Schizosaccharomyces pombe.
Kline L, Nishikawa S, Söll D. Partial purification of RNase P from Schizosaccharomyces pombe. Journal Of Biological Chemistry 1981, 256: 5058-5063. PMID: 6262315, DOI: 10.1016/s0021-9258(19)69366-x.Peer-Reviewed Original ResearchConceptsSimple assay procedureNucleic acid componentsColumn chromatographyReaction productsEnzyme purificationPhosphocellulose column chromatographyAcid componentsSynthetic substratesAssay procedurePurificationAcid solubilityPartial purificationSubstrateYeast RNase PSolubilityChromatographyProductsSeparationPhosphateTrichloroacetic acid solubility
1966
Specificity of sRNA for recognition of codons as studied by the ribosomal binding technique
Söll D, Jones D, Ohtsuka E, Faulkner R, Lohrmann R, Hayatsu H, Khorana H, Cherayil J, Hampel A, Bock R. Specificity of sRNA for recognition of codons as studied by the ribosomal binding technique. Journal Of Molecular Biology 1966, 19: 556-573. PMID: 5338858, DOI: 10.1016/s0022-2836(66)80023-2.Peer-Reviewed Original Research