2017
The stochastic nature of action potential backpropagation in apical tuft dendrites
Short SM, Oikonomou KD, Zhou WL, Acker CD, Popovic MA, Zecevic D, Antic SD. The stochastic nature of action potential backpropagation in apical tuft dendrites. Journal Of Neurophysiology 2017, 118: 1394-1414. PMID: 28566465, PMCID: PMC5558024, DOI: 10.1152/jn.00800.2016.Peer-Reviewed Original ResearchConceptsDendritic CaPyramidal neuronsApical tuftCortical pyramidal neuronsAction potential backpropagationRat brain slicesSpontaneous synaptic inputsDifferent cortical layersVoltage-gated CaAP backpropagationAP burstsGlutamate iontophoresisSynaptic contactsSomatic APsApical trunkAP frequencyBrain slicesDendritic physiologySynaptic inputsCortical layersChannel inactivationLocal NaNeuronsSpike-timing dependent plasticityTrials
2015
Electrical behaviour of dendritic spines as revealed by voltage imaging
Popovic MA, Carnevale N, Rozsa B, Zecevic D. Electrical behaviour of dendritic spines as revealed by voltage imaging. Nature Communications 2015, 6: 8436. PMID: 26436431, PMCID: PMC4594633, DOI: 10.1038/ncomms9436.Peer-Reviewed Original ResearchCombining Membrane Potential Imaging with Other Optical Techniques
Jaafari N, Vogt KE, Saggau P, Leslie LM, Zecevic D, Canepari M. Combining Membrane Potential Imaging with Other Optical Techniques. Advances In Experimental Medicine And Biology 2015, 859: 103-125. PMID: 26238050, PMCID: PMC5675139, DOI: 10.1007/978-3-319-17641-3_4.Peer-Reviewed Original ResearchConceptsMembrane potential imagingOptical techniquesPotential imagingUncaging techniquesChannelrhodopsin stimulationVoltage-sensitive dyeVariety of applicationsSpatial mappingElectrical signalsImportant applicationsVoltageFluorescence intensityImagingParticular moleculeSignalsMembrane potential changesGateNovel investigationOptogeneticsMeasurementsIntensityTechniqueCurrentImaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines
Popovic M, Vogt K, Holthoff K, Konnerth A, Salzberg BM, Grinvald A, Antic SD, Canepari M, Zecevic D. Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines. Advances In Experimental Medicine And Biology 2015, 859: 57-101. PMID: 26238049, PMCID: PMC5671121, DOI: 10.1007/978-3-319-17641-3_3.Peer-Reviewed Original ResearchConceptsIndividual neuronsMembrane potential changesVoltage-sensitive dye recordingAction potential initiationIndividual dendritic spinesSite of originAxon collateralsIndividual nerve cellsMembrane potential transientsVoltage-sensitive dyeDendritic spinesRegional electrical propertiesDendritic treeNerve cellsNeuronal processesSingle axonsPotential initiationComplex operational unitsBehavioral modificationNeuronal network analysisNeuronsInput-output functionMultisite recordings
2013
Combining Ca2+ and membrane potential imaging in single neurons.
Canepari M, Vogt KE, De Waard M, Zecevic D. Combining Ca2+ and membrane potential imaging in single neurons. Cold Spring Harbor Protocols 2013, 2013: 1161-4. PMID: 24298027, PMCID: PMC5225264, DOI: 10.1101/pdb.prot073114.Peer-Reviewed Original Research
2012
Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices.
Popovic M, Gao X, Zecevic D. Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices. Journal Of Visualized Experiments 2012, e4261. PMID: 23222505, PMCID: PMC3565854, DOI: 10.3791/4261.Peer-Reviewed Original ResearchConceptsLaser light sourceSub-micrometer resolutionLight sourceOptical recordingDetectable pharmacological effectsCurrent sensitivityVoltage-sensitive dyeSubthreshold eventsElectrical propertiesDetailed measurementsMultiple-site optical recordingVoltage-sensitive dye recordingFluorescence signalOptimal wavelengthsMembrane potential transientsElectrical signalsIndividual dendritic spinesExperimental evidenceWavelengthMeasurement oneMeasurementsBroadeningDye loadingPotential transientsBrightnessCortical Dendritic Spine Heads Are Not Electrically Isolated by the Spine Neck from Membrane Potential Signals in Parent Dendrites
Popovic MA, Gao X, Carnevale NT, Zecevic D. Cortical Dendritic Spine Heads Are Not Electrically Isolated by the Spine Neck from Membrane Potential Signals in Parent Dendrites. Cerebral Cortex 2012, 24: 385-395. PMID: 23054810, PMCID: PMC3888368, DOI: 10.1093/cercor/bhs320.Peer-Reviewed Original Research
2011
Somatic Membrane Potential and Kv1 Channels Control Spike Repolarization in Cortical Axon Collaterals and Presynaptic Boutons
Foust AJ, Yu Y, Popovic M, Zecevic D, McCormick DA. Somatic Membrane Potential and Kv1 Channels Control Spike Repolarization in Cortical Axon Collaterals and Presynaptic Boutons. Journal Of Neuroscience 2011, 31: 15490-15498. PMID: 22031895, PMCID: PMC3225031, DOI: 10.1523/jneurosci.2752-11.2011.Peer-Reviewed Original ResearchMeSH Keywords4-AminopyridineAnimalsAxonsBiophysical PhenomenaComputer SimulationCrystallinsDose-Response Relationship, DrugElapid VenomsElectric StimulationFemaleGreen Fluorescent ProteinsIn Vitro TechniquesMaleMembrane PotentialsMiceMice, TransgenicModels, NeurologicalMu-CrystallinsNerve NetNeuronsPatch-Clamp TechniquesPotassium Channel BlockersPresynaptic TerminalsShaker Superfamily of Potassium ChannelsSomatosensory CortexStyrenesTetraethylammoniumConceptsAxon collateralsPresynaptic boutonsKv1 channelsSpike repolarizationPresynaptic terminalsSubthreshold depolarizationAction potentialsΑ-dendrotoxinLayer 5 pyramidal cellsIntracortical axon collateralsSynaptic neurotransmitter releaseMouse brain slicesSomatic membrane potentialKv1 subunitsPyramidal cellsSynaptic transmissionBrain slicesVoltage-sensitive dyeLow dosesNeurotransmitter releaseSynaptic strengthBoutonsCollateralsRepolarizationIon channelsThe spatio‐temporal characteristics of action potential initiation in layer 5 pyramidal neurons: a voltage imaging study
Popovic MA, Foust AJ, McCormick DA, Zecevic D. The spatio‐temporal characteristics of action potential initiation in layer 5 pyramidal neurons: a voltage imaging study. The Journal Of Physiology 2011, 589: 4167-4187. PMID: 21669974, PMCID: PMC3180577, DOI: 10.1113/jphysiol.2011.209015.Peer-Reviewed Original ResearchConceptsSpike trigger zoneAction potential initiationAxon initial segmentTrigger zoneLayer 5 pyramidal neuronsPotential initiationLayer 5 pyramidal cellsMature axonsPyramidal neuronsAxonal collateralsPyramidal cellsNeuronal plasticityAction potentialsImmature axonsPotential imaging techniqueAxonsImmunocytochemical dataNeuronal computationSaltatory conductionAP propagationElectrophysiological measuresInitial segmentImaging techniquesCritical roleInitiation
2010
Action Potentials Initiate in the Axon Initial Segment and Propagate through Axon Collaterals Reliably in Cerebellar Purkinje Neurons
Foust A, Popovic M, Zecevic D, McCormick DA. Action Potentials Initiate in the Axon Initial Segment and Propagate through Axon Collaterals Reliably in Cerebellar Purkinje Neurons. Journal Of Neuroscience 2010, 30: 6891-6902. PMID: 20484631, PMCID: PMC2990270, DOI: 10.1523/jneurosci.0552-10.2010.Peer-Reviewed Original ResearchConceptsAxon initial segmentAxon collateralsAction potentialsPurkinje neuronsComplex spikesLocal axon collateralsCerebellar Purkinje neuronsInitial segmentFast action potentialsAxon branch pointsSynaptic inputsVoltage-sensitive dyeCerebellar cortexNeuronal processingSpike initiationDischarge frequencySingle trialCollateralsOutput cellsNeuronsImaging Inhibitory Synaptic Potentials Using Voltage Sensitive Dyes
Canepari M, Willadt S, Zecevic D, Vogt KE. Imaging Inhibitory Synaptic Potentials Using Voltage Sensitive Dyes. Biophysical Journal 2010, 98: 2032-2040. PMID: 20441768, PMCID: PMC2862202, DOI: 10.1016/j.bpj.2010.01.024.Peer-Reviewed Original ResearchConceptsInhibitory postsynaptic potentialsVoltage-sensitive dyeGABAergic inhibitory postsynaptic potentialsCA1 hippocampal pyramidal neuronsPatch electrodeInhibitory synaptic potentialsInhibitory synaptic transmissionHippocampal pyramidal neuronsIntracellular chloride concentrationWhole-cell configurationPyramidal neuronsApical dendritesPostsynaptic potentialsSynaptic potentialsSynaptic transmissionBrain slicesElectrode recordingsNeuronsSensitive dyeVoltage imaging techniqueDendritesMultiple sitesVoltage imagingAxonsRapid time course of action potentials in spines and remote dendrites of mouse visual cortex neurons
Holthoff K, Zecevic D, Konnerth A. Rapid time course of action potentials in spines and remote dendrites of mouse visual cortex neurons. The Journal Of Physiology 2010, 588: 1085-1096. PMID: 20156851, PMCID: PMC2852997, DOI: 10.1113/jphysiol.2009.184960.Peer-Reviewed Original ResearchConceptsRapid time courseAction potentialsDendritic spinesIndividual dendritic spinesLayer 5 pyramidal neuronsTime courseTiming-dependent synaptic plasticityDendritic branchesCentral mammalian neuronsTerminal dendritic branchesVisual cortex neuronsTerminal branchesSomatic action potentialsIndividual spinesRemote dendritesPyramidal neuronsApical dendritesCortex neuronsExcitatory synapsesSupralinear integrationSynaptic inputsVoltage-sensitive dyeJuvenile miceSynaptic plasticityVisual cortex
2009
Wide-Field and Two-Photon Imaging of Brain Activity with Voltage and Calcium-Sensitive Dyes
Homma R, Baker BJ, Jin L, Garaschuk O, Konnerth A, Cohen LB, Bleau CX, Canepari M, Djurisic M, Zecevic D. Wide-Field and Two-Photon Imaging of Brain Activity with Voltage and Calcium-Sensitive Dyes. Methods In Molecular Biology 2009, 489: 43-79. PMID: 18839087, DOI: 10.1007/978-1-59745-543-5_3.Peer-Reviewed Original ResearchConceptsCalcium-sensitive dyeOlfactory bulb glomeruliCell typesBrain activityIndividual cell typesOlfactory receptor neuronsNerve terminalsSynaptic activityVoltage-sensitive dyeSpike activityTwo-photon imagingVivo preparationMammalian brainReceptor neuronsIndividual neuronsIon-sensitive dyesNeuronsTwo-photon microscopyBrainMembrane potentialLow sensitivitySensitive dyeNoseOutput spike trainsSpike trains
2008
Combining Voltage and Calcium Imaging from Neuronal Dendrites
Canepari M, Vogt K, Zecevic D. Combining Voltage and Calcium Imaging from Neuronal Dendrites. Cellular And Molecular Neurobiology 2008, 28: 1079. PMID: 18500551, PMCID: PMC3143714, DOI: 10.1007/s10571-008-9285-y.Peer-Reviewed Original ResearchConceptsHippocampal CA1 pyramidal neuronsCA1 pyramidal neuronsCerebellar Purkinje neuronsOptical signalPyramidal neuronsSame neuronsVoltage-sensitive dyePurkinje neuronsCalcium transientsFura-FFMeaningful new informationNeuronal functionCalcium imagingDendritic integrationNeuronal dendritesFree Ca2NeuronsPhysiological Ca2Membrane potential changesPhysiological changesMembrane potentialCa2Sensitive dyeFunctional Structure of the Mitral Cell Dendritic Tuft in the Rat Olfactory Bulb
Djurisic M, Popovic M, Carnevale N, Zecevic D. Functional Structure of the Mitral Cell Dendritic Tuft in the Rat Olfactory Bulb. Journal Of Neuroscience 2008, 28: 4057-4068. PMID: 18400905, PMCID: PMC6670455, DOI: 10.1523/jneurosci.5296-07.2008.Peer-Reviewed Original ResearchConceptsMitral cellsDendritic tuftsOlfactory bulbGlomerular tuftPrincipal projection neuronsVoltage-sensitive dye recordingRat olfactory bulbSubthreshold synaptic potentialsPrimary dendritesProjection neuronsSynaptic potentialsSame neuronsEPSPsElectrical compartmentsDendritic branchesAnatomical studySynchronized activationDendritic surfaceNeuronsSynapsesCell typesAverage rangePassive electrical propertiesCellsTufts
2007
Dendritic signals from rat hippocampal CA1 pyramidal neurons during coincident pre‐ and post‐synaptic activity: a combined voltage‐ and calcium‐imaging study
Canepari M, Djurisic M, Zecevic D. Dendritic signals from rat hippocampal CA1 pyramidal neurons during coincident pre‐ and post‐synaptic activity: a combined voltage‐ and calcium‐imaging study. The Journal Of Physiology 2007, 580: 463-484. PMID: 17272348, PMCID: PMC2075540, DOI: 10.1113/jphysiol.2006.125005.Peer-Reviewed Original ResearchConceptsLong-term potentiationHippocampal CA1 pyramidal neuronsCA1 pyramidal neuronsPost-synaptic activityPyramidal neuronsRat hippocampal CA1 pyramidal neuronsActivity-dependent long-term synaptic plasticityDendritic action potentialsCalcium imaging studiesLong-term synaptic plasticityDifferent dendritic locationsDendritic signalsDendritic locationMembrane potential transientsNMDA receptorsDendritic arborsPeak amplitudeStimulation protocolSynaptic plasticityAction potentialsMembrane depolarizationDendritic regionsBaselineNeuronsInduction
2005
Imaging of Spiking and Subthreshold Activity of Mitral Cells with Voltage‐Sensitive Dyes
DJURIŠIĆ M, ZEČEVIĆ D. Imaging of Spiking and Subthreshold Activity of Mitral Cells with Voltage‐Sensitive Dyes. Annals Of The New York Academy Of Sciences 2005, 1048: 92-102. PMID: 16154924, DOI: 10.1196/annals.1342.009.Peer-Reviewed Original ResearchConceptsSpike trigger zoneMitral cellsSite of originTrigger zonePrincipal output neuronsTerminal dendritic branchesPrimary dendritesOlfactory nerve stimulationRat olfactory bulbNerve stimulationDendritic tuftsOlfactory bulbVoltage-sensitive dyeAction potentialsNeuronal processesSubthreshold activityIndividual neuronsDendritic branchesSpike initiationIntracellular dyeEPSPsOutput neuronsNeuronsDendritesCellsDeterminants of Low EPSP Attenuation in Primary Dendrites of Mitral Cells: Modeling Study
POPOVIĆ M, DJURIŠIĆ M, ZEČEVIĆ D. Determinants of Low EPSP Attenuation in Primary Dendrites of Mitral Cells: Modeling Study. Annals Of The New York Academy Of Sciences 2005, 1048: 344-348. PMID: 16154948, DOI: 10.1196/annals.1342.033.Peer-Reviewed Original ResearchImaging Brain Activity With Voltage- and Calcium-Sensitive Dyes
Baker BJ, Kosmidis EK, Vucinic D, Falk CX, Cohen LB, Djurisic M, Zecevic D. Imaging Brain Activity With Voltage- and Calcium-Sensitive Dyes. Cellular And Molecular Neurobiology 2005, 25: 245-282. PMID: 16050036, DOI: 10.1007/s10571-005-3059-6.Peer-Reviewed Original ResearchConceptsVoltage-sensitive dyeCalcium-sensitive dyeLight sourceDark noiseVibrational noiseOptical recordingRandom emissionBrain activityFluorescent protein sensorOlfactory receptor neuronsNerve terminalsOlfactory bulbSpike activityNoise ratioCell bodiesReceptor neuronsPhotonsOpticsOptimal signalNeuronsIndividual cell typesKinds of noiseExternal sourcesVoltageCell types
2004
Voltage Imaging from Dendrites of Mitral Cells: EPSP Attenuation and Spike Trigger Zones
Djurisic M, Antic S, Chen WR, Zecevic D. Voltage Imaging from Dendrites of Mitral Cells: EPSP Attenuation and Spike Trigger Zones. Journal Of Neuroscience 2004, 24: 6703-6714. PMID: 15282273, PMCID: PMC6729725, DOI: 10.1523/jneurosci.0307-04.2004.Peer-Reviewed Original ResearchConceptsSpike trigger zoneMitral cellsTrigger zoneAction potentialsPrincipal output neuronsTerminal dendritic branchesPrimary dendritesOlfactory nerve stimulationRat olfactory bulbSite of originNerve stimulationDendritic tuftsSecondary dendritesEPSP attenuationOlfactory bulbSynaptic sitesNeuronal processesIndividual neuronsDendritic branchesSpike initiationIntracellular dyeEPSPsOutput neuronsNeuronsDendrites