2023
Electrical properties of dendritic spines
Zecevic D. Electrical properties of dendritic spines. Biophysical Journal 2023, 122: 4303-4315. PMID: 37837192, PMCID: PMC10698282, DOI: 10.1016/j.bpj.2023.10.008.Peer-Reviewed Original ResearchConceptsDendritic spinesIntracellular calcium concentration changesCortical pyramidal neuronsExcitatory synaptic transmissionCalcium concentration changesBasal dendritesPyramidal neuronsMushroom spinesSpine synapsesSynaptic transmissionBrain slicesVoltage-sensitive dyeNervous systemSpine neckSignificant physiological roleSynaptic signalingTwo-photon uncagingElectrical compartmentsSpineOnly experimental studiesBiochemical compartmentalizationPhysiological roleDifferent preparationsAnatomical structuresAdequate sensitivity
2019
Using holographic illumination to study synaptic signal integration at individual dendritic spines
Weng J, Celis C, Zecevic D. Using holographic illumination to study synaptic signal integration at individual dendritic spines. 2019, 10865: 1086514. DOI: 10.1117/12.2507284.Peer-Reviewed Original ResearchSynaptic signal integrationExcitatory synaptic potentialsDendritic spinesSynaptic potentialsTemporal summationAcute cortical brain slicesIndividual synapsesLayer 5 pyramidal neuronsIndividual excitatory synapsesThin basal dendritesCortical brain slicesVoltage-sensitive dye recordingIndividual dendritic spinesSite of originSensory information processingBasal dendritesPyramidal neuronsExcitatory synapsesBrain slicesRepetitive activationVoltage-sensitive dyeSomatic recordingsSingle synapsesSynaptic signalsIndividual neurons
2017
The stochastic nature of action potential backpropagation in apical tuft dendrites
Short SM, Oikonomou KD, Zhou WL, Acker CD, Popovic MA, Zecevic D, Antic SD. The stochastic nature of action potential backpropagation in apical tuft dendrites. Journal Of Neurophysiology 2017, 118: 1394-1414. PMID: 28566465, PMCID: PMC5558024, DOI: 10.1152/jn.00800.2016.Peer-Reviewed Original ResearchConceptsDendritic CaPyramidal neuronsApical tuftCortical pyramidal neuronsAction potential backpropagationRat brain slicesSpontaneous synaptic inputsDifferent cortical layersVoltage-gated CaAP backpropagationAP burstsGlutamate iontophoresisSynaptic contactsSomatic APsApical trunkAP frequencyBrain slicesDendritic physiologySynaptic inputsCortical layersChannel inactivationLocal NaNeuronsSpike-timing dependent plasticityTrials
2011
The spatio‐temporal characteristics of action potential initiation in layer 5 pyramidal neurons: a voltage imaging study
Popovic MA, Foust AJ, McCormick DA, Zecevic D. The spatio‐temporal characteristics of action potential initiation in layer 5 pyramidal neurons: a voltage imaging study. The Journal Of Physiology 2011, 589: 4167-4187. PMID: 21669974, PMCID: PMC3180577, DOI: 10.1113/jphysiol.2011.209015.Peer-Reviewed Original ResearchConceptsSpike trigger zoneAction potential initiationAxon initial segmentTrigger zoneLayer 5 pyramidal neuronsPotential initiationLayer 5 pyramidal cellsMature axonsPyramidal neuronsAxonal collateralsPyramidal cellsNeuronal plasticityAction potentialsImmature axonsPotential imaging techniqueAxonsImmunocytochemical dataNeuronal computationSaltatory conductionAP propagationElectrophysiological measuresInitial segmentImaging techniquesCritical roleInitiation
2010
Imaging Inhibitory Synaptic Potentials Using Voltage Sensitive Dyes
Canepari M, Willadt S, Zecevic D, Vogt KE. Imaging Inhibitory Synaptic Potentials Using Voltage Sensitive Dyes. Biophysical Journal 2010, 98: 2032-2040. PMID: 20441768, PMCID: PMC2862202, DOI: 10.1016/j.bpj.2010.01.024.Peer-Reviewed Original ResearchConceptsInhibitory postsynaptic potentialsVoltage-sensitive dyeGABAergic inhibitory postsynaptic potentialsCA1 hippocampal pyramidal neuronsPatch electrodeInhibitory synaptic potentialsInhibitory synaptic transmissionHippocampal pyramidal neuronsIntracellular chloride concentrationWhole-cell configurationPyramidal neuronsApical dendritesPostsynaptic potentialsSynaptic potentialsSynaptic transmissionBrain slicesElectrode recordingsNeuronsSensitive dyeVoltage imaging techniqueDendritesMultiple sitesVoltage imagingAxonsRapid time course of action potentials in spines and remote dendrites of mouse visual cortex neurons
Holthoff K, Zecevic D, Konnerth A. Rapid time course of action potentials in spines and remote dendrites of mouse visual cortex neurons. The Journal Of Physiology 2010, 588: 1085-1096. PMID: 20156851, PMCID: PMC2852997, DOI: 10.1113/jphysiol.2009.184960.Peer-Reviewed Original ResearchConceptsRapid time courseAction potentialsDendritic spinesIndividual dendritic spinesLayer 5 pyramidal neuronsTime courseTiming-dependent synaptic plasticityDendritic branchesCentral mammalian neuronsTerminal dendritic branchesVisual cortex neuronsTerminal branchesSomatic action potentialsIndividual spinesRemote dendritesPyramidal neuronsApical dendritesCortex neuronsExcitatory synapsesSupralinear integrationSynaptic inputsVoltage-sensitive dyeJuvenile miceSynaptic plasticityVisual cortex
2008
Combining Voltage and Calcium Imaging from Neuronal Dendrites
Canepari M, Vogt K, Zecevic D. Combining Voltage and Calcium Imaging from Neuronal Dendrites. Cellular And Molecular Neurobiology 2008, 28: 1079. PMID: 18500551, PMCID: PMC3143714, DOI: 10.1007/s10571-008-9285-y.Peer-Reviewed Original ResearchConceptsHippocampal CA1 pyramidal neuronsCA1 pyramidal neuronsCerebellar Purkinje neuronsOptical signalPyramidal neuronsSame neuronsVoltage-sensitive dyePurkinje neuronsCalcium transientsFura-FFMeaningful new informationNeuronal functionCalcium imagingDendritic integrationNeuronal dendritesFree Ca2NeuronsPhysiological Ca2Membrane potential changesPhysiological changesMembrane potentialCa2Sensitive dye
2007
Dendritic signals from rat hippocampal CA1 pyramidal neurons during coincident pre‐ and post‐synaptic activity: a combined voltage‐ and calcium‐imaging study
Canepari M, Djurisic M, Zecevic D. Dendritic signals from rat hippocampal CA1 pyramidal neurons during coincident pre‐ and post‐synaptic activity: a combined voltage‐ and calcium‐imaging study. The Journal Of Physiology 2007, 580: 463-484. PMID: 17272348, PMCID: PMC2075540, DOI: 10.1113/jphysiol.2006.125005.Peer-Reviewed Original ResearchConceptsLong-term potentiationHippocampal CA1 pyramidal neuronsCA1 pyramidal neuronsPost-synaptic activityPyramidal neuronsRat hippocampal CA1 pyramidal neuronsActivity-dependent long-term synaptic plasticityDendritic action potentialsCalcium imaging studiesLong-term synaptic plasticityDifferent dendritic locationsDendritic signalsDendritic locationMembrane potential transientsNMDA receptorsDendritic arborsPeak amplitudeStimulation protocolSynaptic plasticityAction potentialsMembrane depolarizationDendritic regionsBaselineNeuronsInduction
1999
Fast Optical Recordings of Membrane Potential Changes From Dendrites of Pyramidal Neurons
Antic S, Major G, Zecevic D. Fast Optical Recordings of Membrane Potential Changes From Dendrites of Pyramidal Neurons. Journal Of Neurophysiology 1999, 82: 1615-1621. PMID: 10482775, DOI: 10.1152/jn.1999.82.3.1615.Peer-Reviewed Original ResearchConceptsBasolateral dendritesApical dendritesBrain slicesVoltage-sensitive dyeNeuronal processesPyramidal neuronsMembrane potential transientsPharmacological effectsSynaptic stimulationAction potentialsInvertebrate neuronsNeuronsPatch electrodeSingle neuronsElectrical activityMembrane potential changesFast optical recordingDistal processesVertebrate neuronsOptical recordingDendritesH incubation periodStimulationApical processesRecordings