2015
Combining Membrane Potential Imaging with Other Optical Techniques
Jaafari N, Vogt KE, Saggau P, Leslie LM, Zecevic D, Canepari M. Combining Membrane Potential Imaging with Other Optical Techniques. Advances In Experimental Medicine And Biology 2015, 859: 103-125. PMID: 26238050, PMCID: PMC5675139, DOI: 10.1007/978-3-319-17641-3_4.Peer-Reviewed Original ResearchConceptsMembrane potential imagingOptical techniquesPotential imagingUncaging techniquesChannelrhodopsin stimulationVoltage-sensitive dyeVariety of applicationsSpatial mappingElectrical signalsImportant applicationsVoltageFluorescence intensityImagingParticular moleculeSignalsMembrane potential changesGateNovel investigationOptogeneticsMeasurementsIntensityTechniqueCurrentImaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines
Popovic M, Vogt K, Holthoff K, Konnerth A, Salzberg BM, Grinvald A, Antic SD, Canepari M, Zecevic D. Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines. Advances In Experimental Medicine And Biology 2015, 859: 57-101. PMID: 26238049, PMCID: PMC5671121, DOI: 10.1007/978-3-319-17641-3_3.Peer-Reviewed Original ResearchConceptsIndividual neuronsMembrane potential changesVoltage-sensitive dye recordingAction potential initiationIndividual dendritic spinesSite of originAxon collateralsIndividual nerve cellsMembrane potential transientsVoltage-sensitive dyeDendritic spinesRegional electrical propertiesDendritic treeNerve cellsNeuronal processesSingle axonsPotential initiationComplex operational unitsBehavioral modificationNeuronal network analysisNeuronsInput-output functionMultisite recordings
2012
Cortical Dendritic Spine Heads Are Not Electrically Isolated by the Spine Neck from Membrane Potential Signals in Parent Dendrites
Popovic MA, Gao X, Carnevale NT, Zecevic D. Cortical Dendritic Spine Heads Are Not Electrically Isolated by the Spine Neck from Membrane Potential Signals in Parent Dendrites. Cerebral Cortex 2012, 24: 385-395. PMID: 23054810, PMCID: PMC3888368, DOI: 10.1093/cercor/bhs320.Peer-Reviewed Original Research
2010
Combined Voltage and Calcium Imaging and Signal Calibration
Canepari M, Saggau P, Zecevic D. Combined Voltage and Calcium Imaging and Signal Calibration. 2010, 43-52. DOI: 10.1007/978-1-4419-6558-5_4.Peer-Reviewed Original ResearchOptical measurementsVoltage-sensitive dye signalsVoltage imagingAbsolute scaleCapability of voltageVoltage-sensitive dyeFluorescent voltage-sensitive dyeSignal calibrationDye signalsSpatial mappingVoltageMeasurementsVoltage signalImagingMembrane potential changesSignalsCalibrationCombined voltageFundamental problemImaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines
Canepari M, Popovic M, Vogt K, Holthoff K, Konnerth A, Salzberg B, Grinvald A, Antic S, Zecevic D. Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines. 2010, 25-41. DOI: 10.1007/978-1-4419-6558-5_3.Peer-Reviewed Original ResearchIndividual neuronsMembrane potential changesVoltage-sensitive dye recordingAction potential initiationIndividual dendritic spinesSite of originAxon collateralsIndividual nerve cellsMembrane potential transientsVoltage-sensitive dyeDendritic spinesRegional electrical propertiesDendritic treeNerve cellsNeuronal processesSingle axonsPotential initiationComplex operational unitsBehavioral modificationNeuronal network analysisNeuronsInput-output functionMultisite recordingsSubthreshold eventsSpine
2008
Combining Voltage and Calcium Imaging from Neuronal Dendrites
Canepari M, Vogt K, Zecevic D. Combining Voltage and Calcium Imaging from Neuronal Dendrites. Cellular And Molecular Neurobiology 2008, 28: 1079. PMID: 18500551, PMCID: PMC3143714, DOI: 10.1007/s10571-008-9285-y.Peer-Reviewed Original ResearchConceptsHippocampal CA1 pyramidal neuronsCA1 pyramidal neuronsCerebellar Purkinje neuronsOptical signalPyramidal neuronsSame neuronsVoltage-sensitive dyePurkinje neuronsCalcium transientsFura-FFMeaningful new informationNeuronal functionCalcium imagingDendritic integrationNeuronal dendritesFree Ca2NeuronsPhysiological Ca2Membrane potential changesPhysiological changesMembrane potentialCa2Sensitive dye
1999
Fast Optical Recordings of Membrane Potential Changes From Dendrites of Pyramidal Neurons
Antic S, Major G, Zecevic D. Fast Optical Recordings of Membrane Potential Changes From Dendrites of Pyramidal Neurons. Journal Of Neurophysiology 1999, 82: 1615-1621. PMID: 10482775, DOI: 10.1152/jn.1999.82.3.1615.Peer-Reviewed Original ResearchConceptsBasolateral dendritesApical dendritesBrain slicesVoltage-sensitive dyeNeuronal processesPyramidal neuronsMembrane potential transientsPharmacological effectsSynaptic stimulationAction potentialsInvertebrate neuronsNeuronsPatch electrodeSingle neuronsElectrical activityMembrane potential changesFast optical recordingDistal processesVertebrate neuronsOptical recordingDendritesH incubation periodStimulationApical processesRecordingsChapter sixteen Imaging Membrane Potential Changes in Individual Neurons
Antić S, Zečević D. Chapter sixteen Imaging Membrane Potential Changes in Individual Neurons. 1999, 238-248. DOI: 10.1016/b978-012447836-7/50018-x.Peer-Reviewed Original ResearchVoltage-sensitive dyeIndividual neuronsSingle neuronsVoltage-sensitive dye recordingSpatial resolutionIndirect optical measurementsDirect electrical recordingOptical signalGood spatial resolutionOptical measurementsIndividual nerve cellsPresent sensitivityFunctional neuronal networksNeuronal dendritic treeOptical recordingDendritic treeNerve cellsDye injectionNeuronsIntracellular applicationMembrane potential changesNeuronal networksMultisite recordingsElectrical recordingsLocal modulation