2004
Cloning of Apoptosis-Related Genes by Representational Difference Analysis of cDNA
Hubank M, Bryntesson F, Regan J, Schatz DG. Cloning of Apoptosis-Related Genes by Representational Difference Analysis of cDNA. Methods In Molecular Biology 2004, 282: 255-273. PMID: 15105570, DOI: 10.1385/1-59259-812-9:255.Peer-Reviewed Original ResearchConceptsRepresentational difference analysisApoptosis-related genesComplementary DNA representational difference analysisCDNA-RDAGene expressionDifferential gene expression analysisIdentification of genesKey target genesExpression levelsGene expression analysisLow-abundance transcriptsGene microarray analysisModel organismsTranscriptional differencesTarget genesExpression analysisDifferential displayMicroarray analysisGene microarrayCDNA fragmentsCDNA ampliconsGenesBase pairsSerial analysisPolymerase chain reaction amplification
1996
Productive T-cell receptor beta-chain gene rearrangement: coincident regulation of cell cycle and clonality during development in vivo.
Hoffman ES, Passoni L, Crompton T, Leu TM, Schatz DG, Koff A, Owen MJ, Hayday AC. Productive T-cell receptor beta-chain gene rearrangement: coincident regulation of cell cycle and clonality during development in vivo. Genes & Development 1996, 10: 948-962. PMID: 8608942, DOI: 10.1101/gad.10.8.948.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, Differentiation, T-LymphocyteCell CycleCell SeparationClone CellsCyclinsDNA-Binding ProteinsFemaleFlow CytometryFluorescent Antibody Technique, IndirectGene Expression Regulation, DevelopmentalGene Rearrangement, beta-Chain T-Cell Antigen ReceptorHomeodomain ProteinsHyaluronan ReceptorsMiceMice, Inbred C57BLProteinsReceptors, Antigen, T-Cell, alpha-betaReceptors, Interleukin-2Retinoblastoma ProteinRNA, MessengerThymus GlandT-LymphocytesConceptsTCRbeta chain genesBeta selectionT-cell receptor beta-chain locusChain geneTCRbeta chain gene rearrangementAlpha beta T cell developmentProductive gene rearrangementHyperphosphorylation of RbGene rearrangementsTCR gene rearrangementsTransition of thymocytesTCRbeta gene rearrangementT cell developmentRegulation of p27Coincident regulationBeta-chain locusPopulation of cellsTCR lociCell cycleCdc2 activityCDK2 activityTCRbeta selectionCyclin AThymocyte expansionCell phenotype