2020
Nucleolar localization of RAG1 modulates V(D)J recombination activity
Brecht RM, Liu CC, Beilinson HA, Khitun A, Slavoff SA, Schatz DG. Nucleolar localization of RAG1 modulates V(D)J recombination activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 4300-4309. PMID: 32047031, PMCID: PMC7049140, DOI: 10.1073/pnas.1920021117.Peer-Reviewed Original ResearchConceptsNucleolar localizationProximity-dependent biotin identificationRecombination activityDisruption of nucleoliDiscrete gene segmentsAntigen receptor lociPre-B cell linesNegative regulatory mechanismsN-terminal regionAmino acids 216Biotin identificationLocalization motifNucleolar associationProtein complexesNucleolar proteinsNucleolar sequestrationT-cell receptor genesRegulatory mechanismsNucleolar markerReceptor locusEfficient egressRAG1Amino acidsGene segmentsReceptor gene
1997
Identification of V(D)J recombination coding end intermediates in normal thymocytes 11Edited by K. Yamamoto
Livák F, Schatz D. Identification of V(D)J recombination coding end intermediates in normal thymocytes 11Edited by K. Yamamoto. Journal Of Molecular Biology 1997, 267: 1-9. PMID: 9096202, DOI: 10.1006/jmbi.1996.0834.Peer-Reviewed Original ResearchConceptsRecombination signal sequencesNormal lymphoid precursorsSignal endsJ alpha genesPre-B cell linesGene rearrangement processDouble-strand breaksNormal murine thymocytesSignal sequenceLymphoid precursorsK. YamamotoAlpha geneFirst direct demonstrationHairpin structureLow abundanceStrand breaksGene segmentsCell linesAntigen receptorMurine thymocytesRecombinationDirect demonstrationVivoJoint formationNormal thymocytes
1987
Increased Frequency of N-Region Insertion in a Murine Pre-B-Cell Line Infected with a Terminal Deoxynucleotidyl Transferase Retroviral Expression Vector
Landau N, Schatz D, Rosa M, Baltimore D. Increased Frequency of N-Region Insertion in a Murine Pre-B-Cell Line Infected with a Terminal Deoxynucleotidyl Transferase Retroviral Expression Vector. Molecular And Cellular Biology 1987, 7: 3237-3243. DOI: 10.1128/mcb.7.9.3237-3243.1987.Peer-Reviewed Original ResearchPre-B cell linesN-region insertionsRetroviral expression vectorExpression vectorCell linesMurine pre-B cell lineImmunoglobulin light chain genesNucleotide sequence analysisLight chain genesV-J junctionGene junctionSequence analysisImmunoglobulin genesGenesRearrangement substratesTerminal deoxynucleotidyl transferaseContinuous rearrangementApparent absenceJunctional sitesProvirusDeoxynucleotidyl transferaseRearrangementControl vectorN regionInsertionIncreased frequency of N-region insertion in a murine pre-B-cell line infected with a terminal deoxynucleotidyl transferase retroviral expression vector.
Landau NR, Schatz DG, Rosa M, Baltimore D. Increased frequency of N-region insertion in a murine pre-B-cell line infected with a terminal deoxynucleotidyl transferase retroviral expression vector. Molecular And Cellular Biology 1987, 7: 3237-3243. PMID: 3118194, PMCID: PMC367960, DOI: 10.1128/mcb.7.9.3237.Peer-Reviewed Original ResearchConceptsPre-B cell linesN-region insertionsRetroviral expression vectorExpression vectorCell linesMurine pre-B cell lineImmunoglobulin light chain genesNucleotide sequence analysisLight chain genesV-J junctionGene junctionSequence analysisImmunoglobulin genesGenesRearrangement substratesTerminal deoxynucleotidyl transferaseContinuous rearrangementApparent absenceJunctional sitesProvirusDeoxynucleotidyl transferaseRearrangementControl vectorN regionInsertion