2018
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization.
Memi F, Tirziu D, Papangeli I. Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization. Journal Of Visualized Experiments 2018 PMID: 30272664, PMCID: PMC6235194, DOI: 10.3791/57920.Peer-Reviewed Original ResearchConceptsMouse heart sectionsMiRNA expression profilingMiRNA-182Relevant protein expressionMiRNAs of interestMiRNA transcriptsT proteinRNA transcriptsExpression profilingMicro-RNAsDetailed protocolSitu hybridization techniqueCardiac troponin T proteinsSitu hybridizationLNA probesProtein expressionTroponin T proteinsTranscriptsHybridization techniqueCardiomyocyte cellsProteinExpressionFluorescent stainingMiRNA detectionRNAs
2013
NO triggers RGS4 degradation to coordinate angiogenesis and cardiomyocyte growth
Jaba IM, Zhuang ZW, Li N, Jiang Y, Martin KA, Sinusas AJ, Papademetris X, Simons M, Sessa WC, Young LH, Tirziu D. NO triggers RGS4 degradation to coordinate angiogenesis and cardiomyocyte growth. Journal Of Clinical Investigation 2013, 123: 1718-1731. PMID: 23454748, PMCID: PMC3613910, DOI: 10.1172/jci65112.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, BiologicalAnimalsCell EnlargementCells, CulturedCoronary VesselsEndothelium, VascularHeart VentriclesMechanistic Target of Rapamycin Complex 1MiceMice, Inbred C57BLMice, TransgenicMultiprotein ComplexesMyocytes, CardiacNeovascularization, PhysiologicNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthasePlacenta Growth FactorPregnancy ProteinsProteinsProteolysisProto-Oncogene Proteins c-aktRatsRats, Sprague-DawleyRGS ProteinsSignal TransductionTOR Serine-Threonine KinasesConceptsCardiomyocyte growthAkt/mTORC1 signalingNovel NO-dependent mechanismProteasomal degradationCoordination of angiogenesisMTORC1 signalingConditional overexpressionMurine cardiac tissueG proteinsTransgenic expressionAkt/Physiological mechanismsMyocyte growthVessel growthGrowth factorTransgenic miceHypertrophic responseAngiogenesisKnockout miceMyocardial hypertrophyExpressionGrowthCardiac hypertrophyNOS inhibitor L-NAMEInduction