Featured Publications
Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin.
Baker CA, Elyada YM, Parra A, Bolton MM. Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin. ELife 2016, 5 PMID: 27525487, PMCID: PMC5001837, DOI: 10.7554/eLife.14193.Peer-Reviewed Original ResearchSparse recurrent excitatory connectivity in the microcircuit of the adult mouse and human cortex
Seeman SC, Campagnola L, Davoudian PA, Hoggarth A, Hage TA, Bosma-Moody A, Baker CA, Lee JH, Mihalas S, Teeter C, Ko AL, Ojemann JG, Gwinn RP, Silbergeld DL, Cobbs C, Phillips J, Lein E, Murphy G, Koch C, Zeng H, Jarsky T. Sparse recurrent excitatory connectivity in the microcircuit of the adult mouse and human cortex. ELife 2018, 7: e37349. PMID: 30256194, PMCID: PMC6158007, DOI: 10.7554/elife.37349.Peer-Reviewed Original ResearchConceptsRecurrent excitatory connectivityExcitatory connectivityAdult mouse primary visual cortexMouse primary visual cortexHuman cortexLayer 2/3 neuronsClasses of neuronsPrimary visual cortexShort-term depressionBody of evidenceExcitatory neuronsAdult miceVisual cortexSynaptic signalingTwo-photon optogeneticsCell classesCortical networksCortical microcircuitsCortexNeuronsFuture studiesMiceMicrocircuitsSynapsesSynaptic connectivity to L2/3 of primary visual cortex measured by two-photon optogenetic stimulation.
Hage TA, Bosma-Moody A, Baker CA, Kratz MB, Campagnola L, Jarsky T, Zeng H, Murphy GJ. Synaptic connectivity to L2/3 of primary visual cortex measured by two-photon optogenetic stimulation. Elife 2022, 11 PMID: 35060903, DOI: 10.7554/eLife.71103.Peer-Reviewed Original ResearchPaclitaxel effects on axonal localization and vesicular trafficking of NaV1.8
Baker C, Tyagi S, Higerd-Rusli G, Liu S, Zhao P, Dib-Hajj F, Waxman S, Dib-Hajj S. Paclitaxel effects on axonal localization and vesicular trafficking of NaV1.8. Frontiers In Molecular Neuroscience 2023, 16: 1130123. PMID: 36860665, PMCID: PMC9970094, DOI: 10.3389/fnmol.2023.1130123.Peer-Reviewed Original ResearchChemotherapy-induced peripheral neuropathyDorsal root gangliaPTX treatmentDRG axonsEffect of paclitaxelVoltage-gated sodium channel NaPain syndromePeripheral neuropathyDRG neuronsSodium channel NaRoot gangliaCell cycle arrestNeuronal somataSensory neuronsSide effectsTherapeutic targetingTumor growthPaclitaxel effectAntineoplastic agentsAxonal localizationPaclitaxelNumber of NaAxonal compartmentAxonsChannel NaUnique inflammatory RNA profiles of microglia in Creutzfeldt–Jakob disease
Baker CA, Manuelidis L. Unique inflammatory RNA profiles of microglia in Creutzfeldt–Jakob disease. Proceedings Of The National Academy Of Sciences Of The United States Of America 2003, 100: 675-679. PMID: 12525699, PMCID: PMC141055, DOI: 10.1073/pnas.0237313100.Peer-Reviewed Original ResearchConceptsCreutzfeldt-Jakob diseaseMyeloid cellsUninfected microgliaInflammatory pathwaysMicroglial cellsIFN-gammaInflammatory stimuliMicrogliaInflammatory genesProtein pathologyInfectious agentsPreclinical diagnosisNeurodegenerative diseasesTransmissible spongiformDiseaseBrainPrP amyloidRNA profilesExpression profilesCellsCDNA arraysPrevious studiesInfectionDiagnosisPathology
2024
Real-time imaging of axonal membrane protein life cycles
Tyagi S, Higerd-Rusli G, Akin E, Baker C, Liu S, Dib-Hajj F, Waxman S, Dib-Hajj S. Real-time imaging of axonal membrane protein life cycles. Nature Protocols 2024, 19: 2771-2802. PMID: 38831222, DOI: 10.1038/s41596-024-00997-x.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMembrane proteinsRecycling of membrane proteinsProtein subcellular localizationMembrane protein homeostasisMembrane protein traffickingEngineered membrane proteinsMultiple membrane proteinsSelf-labeling tagsCell culturesProtein traffickingProtein tagsSubcellular localizationProtein homeostasisSpatiotemporal regulationCellular processesMultiple proteinsSubcellular distributionVesicular packagingThroughput mannerProteinNeuronal compartmentsDistal axonsProtein spatial organizationFluorescent labelingNeuronal culturesBeyond antiviral: role of IFN-I in brain development
Baker C, Iwasaki A. Beyond antiviral: role of IFN-I in brain development. Trends In Immunology 2024, 45: 322-324. PMID: 38644134, DOI: 10.1016/j.it.2024.04.004.Commentaries, Editorials and LettersSpying on specific splicing in spinal nerve injury.
Baker CA. Spying on specific splicing in spinal nerve injury. Neurotherapeutics 2024, 21: e00315. PMID: 38216398, DOI: 10.1016/j.neurot.2024.e00315.Commentaries, Editorials and Letters
2023
Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator.
Abdelfattah AS, Zheng J, Singh A, Huang YC, Reep D, Tsegaye G, Tsang A, Arthur BJ, Rehorova M, Olson CVL, Shuai Y, Zhang L, Fu TM, Milkie DE, Moya MV, Weber TD, Lemire AL, Baker CA, Falco N, Zheng Q, Grimm JB, Yip MC, Walpita D, Chase M, Campagnola L, Murphy GJ, Wong AM, Forest CR, Mertz J, Economo MN, Turner GC, Koyama M, Lin BJ, Betzig E, Novak O, Lavis LD, Svoboda K, Korff W, Chen TW, Schreiter ER, Hasseman JP, Kolb I. Sensitivity optimization of a rhodopsin-based fluorescent voltage indicator. Neuron 2023, 111: 1547-1563.e9. PMID: 37015225, DOI: 10.1016/j.neuron.2023.03.009.Peer-Reviewed Original ResearchInflammation differentially controls transport of depolarizing Nav versus hyperpolarizing Kv channels to drive rat nociceptor activity
Higerd-Rusli G, Tyagi S, Baker C, Liu S, Dib-Hajj F, Dib-Hajj S, Waxman S. Inflammation differentially controls transport of depolarizing Nav versus hyperpolarizing Kv channels to drive rat nociceptor activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2215417120. PMID: 36897973, PMCID: PMC10089179, DOI: 10.1073/pnas.2215417120.Peer-Reviewed Original ResearchConceptsCell biological mechanismsAxonal surfaceLive-cell imagingIon channel traffickingAnterograde transport vesiclesTransport vesiclesInflammatory mediatorsChannel traffickingPlasma membraneVesicular loadingIon channelsKv channelsPotential therapeutic targetPotassium channel KSodium channel NaTraffickingBiological mechanismsTherapeutic targetAbundanceRetrograde transportDistal axonsChannel NaInflammatory painNociceptor activityAxonal transport
2021
Autonomous Purkinje cell activation instructs bidirectional motor learning through evoked dendritic calcium signaling.
Bonnan A, Rowan MMJ, Baker CA, Bolton MM, Christie JM. Autonomous Purkinje cell activation instructs bidirectional motor learning through evoked dendritic calcium signaling. Nature Communications 2021, 12: 2153. PMID: 33846328, PMCID: PMC8042043, DOI: 10.1038/s41467-021-22405-8.Peer-Reviewed Original Research
2019
Regional Specialization of Pyramidal Neuron Morphology and Physiology in the Tree Shrew Neocortex.
Parra A, Baker CA, Bolton MM. Regional Specialization of Pyramidal Neuron Morphology and Physiology in the Tree Shrew Neocortex. Cerebral Cortex (New York, N.Y. : 1991) 2019, 29: 4488-4505. PMID: 30715235, DOI: 10.1093/cercor/bhy326.Peer-Reviewed Original Research
2018
A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality.
Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA, Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT, Sunkin SM, Lecoq J, Lin MZ, Boyden ES, Murphy GJ, da Costa NM, Waters J, Li L, Tasic B, Zeng H. A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality. Cell 2018, 174: 465-480.e22. PMID: 30007418, PMCID: PMC6086366, DOI: 10.1016/j.cell.2018.06.035.Peer-Reviewed Original ResearchTwo-Photon Bidirectional Control and Imaging of Neuronal Excitability with High Spatial Resolution In Vivo.
Forli A, Vecchia D, Binini N, Succol F, Bovetti S, Moretti C, Nespoli F, Mahn M, Baker CA, Bolton MM, Yizhar O, Fellin T. Two-Photon Bidirectional Control and Imaging of Neuronal Excitability with High Spatial Resolution In Vivo. Cell Reports 2018, 22: 3087-3098. PMID: 29539433, PMCID: PMC5863087, DOI: 10.1016/j.celrep.2018.02.063.Peer-Reviewed Original Research
2011
Abrogation of complex glycosylation by swainsonine results in strain- and cell-specific inhibition of prion replication.
Browning S, Baker CA, Smith E, Mahal SP, Herva ME, Demczyk CA, Li J, Weissmann C. Abrogation of complex glycosylation by swainsonine results in strain- and cell-specific inhibition of prion replication. J Biol Chem 2011, 286: 40962-73. PMID: 21930694, DOI: 10.1074/jbc.M111.283978.Peer-Reviewed Original Research
2007
Prion strain discrimination in cell culture: the cell panel assay.
Mahal SP, Baker CA, Demczyk CA, Smith EW, Julius C, Weissmann C. Prion strain discrimination in cell culture: the cell panel assay. Proc Natl Acad Sci U S A 2007, 104: 20908-13. PMID: 18077360, DOI: 10.1073/pnas.0710054104.Peer-Reviewed Original Research
2004
New molecular markers of early and progressive CJD brain infection
Lu ZY, Baker CA, Manuelidis L. New molecular markers of early and progressive CJD brain infection. Journal Of Cellular Biochemistry 2004, 93: 644-652. PMID: 15660413, DOI: 10.1002/jcb.20220.Peer-Reviewed Original ResearchConceptsCreutzfeldt-Jakob diseaseBrain infectionTransmissible spongiform encephalopathiesHuman Creutzfeldt-Jakob diseaseAccessible peripheral tissuesL-selectin mRNASerum amyloid A3Progressive diseaseInflammatory pathwaysMIP-1alphaMIP-1betaIntracerebral inoculationPeripheral tissuesEarly diagnosisTSE infectionInfectious componentNormal brainTSE strainsInfectious agentsMyeloid cellsSimilar sequential changesImmune systemMouse brainTSE agentsNew molecular markersEarly induction of interferon-responsive mRNAs in Creutzfeldt-Jakob disease
Baker CA, Lu ZY, Manuelidis L. Early induction of interferon-responsive mRNAs in Creutzfeldt-Jakob disease. Journal Of NeuroVirology 2004, 10: 29-40. PMID: 14982726, PMCID: PMC4624297, DOI: 10.1080/13550280490261761.Peer-Reviewed Original ResearchConceptsCreutzfeldt-Jakob diseaseCJD agentCJD brainsInterferon-sensitive genesImmune responseForeign infectious agentsHost immune responseAbnormal prion proteinHost defense mechanismsMicroglial responseInflammatory pathwaysClinical signsPure microgliaViral infectionInfectious agentsWhole brainImmune recognitionPersistent virusesInterferon responsePreclinical diagnosisInfectionEarly inductionBrainMicrogliaGene expression profiles
2002
Microglia from Creutzfeldt-Jakob Disease-Infected Brains Are Infectious and Show Specific mRNA Activation Profiles
Baker CA, Martin D, Manuelidis L. Microglia from Creutzfeldt-Jakob Disease-Infected Brains Are Infectious and Show Specific mRNA Activation Profiles. Journal Of Virology 2002, 76: 10905-10913. PMID: 12368333, PMCID: PMC136595, DOI: 10.1128/jvi.76.21.10905-10913.2002.Peer-Reviewed Original ResearchMeSH KeywordsAmyloid beta-PeptidesAnimalsAntigens, CDAntigens, Differentiation, MyelomonocyticBrainCD40 AntigensChemokine CXCL10Chemokines, CXCCreutzfeldt-Jakob SyndromeGene ExpressionGene Expression ProfilingInterleukin-1Leukocyte Common AntigensLipopolysaccharidesMiceMicrogliaReceptor, Macrophage Colony-Stimulating FactorReceptors, CCR5RNA, MessengerTumor Necrosis Factor-alphaConceptsCreutzfeldt-Jakob diseaseCJD agentInfectious agentsCJD-infected miceB lymphocyte chemoattractantCytokine interleukin-1betaResult of infectionPathological prion proteinBrain cell typesMicroglial studiesMicroglial changesMicroglial markersReverse transcription-PCRAntigen presentationInterleukin-1betaBrain homogenatesMicrogliaPrP levelsBacterial lipopolysaccharideCellular activationPathological PrPActivated cellsBovine spongiformSpongiform encephalopathiesMolecular pathways
1999
Microglial Activation Varies in Different Models of Creutzfeldt-Jakob Disease
Baker C, Lu Z, Zaitsev I, Manuelidis L. Microglial Activation Varies in Different Models of Creutzfeldt-Jakob Disease. Journal Of Virology 1999, 73: 5089-5097. PMID: 10233972, PMCID: PMC112554, DOI: 10.1128/jvi.73.6.5089-5097.1999.Peer-Reviewed Original ResearchConceptsCreutzfeldt-Jakob diseaseLysosomal protease cathepsin SCathepsin SMRNA expressionAbnormal PrP formationHost mRNA expressionUpregulation of CCR5Chemokine receptor CCR5Glial cell involvementGrowth factor beta1Microglial transcriptsNeuronal destructionProtease cathepsin SMicroglial activationAmyloid depositionPathogenetic pathwaysReceptor CCR5Cell involvementRodent modelsChronic reservoirDistinctive neuropathologyPleiotropic cytokineTGF-beta1Primary sitePrP pathology