2023
Two cold shock domain containing proteins trigger the development of infectious Trypanosoma brucei
Toh J, Nkouawa A, Dong G, Kolev N, Tschudi C. Two cold shock domain containing proteins trigger the development of infectious Trypanosoma brucei. PLOS Pathogens 2023, 19: e1011438. PMID: 37276216, PMCID: PMC10270622, DOI: 10.1371/journal.ppat.1011438.Peer-Reviewed Original ResearchConceptsCold shock domainMetacyclic parasitesRegulation of transcriptionMicrotubule-based movementProtozoan parasite Trypanosoma bruceiCold shock proteinsRNA-binding proteinC-terminal domainFamily of DNAN-terminal domainDNA damage repairParasite Trypanosoma bruceiSpecific binding motifAmino acid residuesMammalian infectivityNucleocytoplasmic transportDevelopmental programProcyclic parasitesTrypanosoma bruceiCSD2CSD1MRNA stabilityBiological functionsBinding motifBiological processes
2017
The proteome and transcriptome of the infectious metacyclic form of Trypanosoma brucei define quiescent cells primed for mammalian invasion
Christiano R, Kolev NG, Shi H, Ullu E, Walther TC, Tschudi C. The proteome and transcriptome of the infectious metacyclic form of Trypanosoma brucei define quiescent cells primed for mammalian invasion. Molecular Microbiology 2017, 106: 74-92. PMID: 28742275, PMCID: PMC5607103, DOI: 10.1111/mmi.13754.Peer-Reviewed Original ResearchConceptsInfectious metacyclic formsTsetse fly vectorMetacyclic formsVariant surface glycoprotein expression sitesQuiescent cellsGene expression profilesFly vectorsAvailability of nutrientsMammalian invasionsCell surface componentsCell divisionProcyclic trypanosomesMammalian hostsTrypanosoma bruceiMRNA sequencingExpression profilesExpression sitesMetabolic enzymesBloodstream formsInfectious metacyclicsProtein 6TranscriptomeTsetse fliesProteomeProtein levels
1998
Double-stranded RNA induces mRNA degradation in Trypanosoma brucei
Ngô H, Tschudi C, Gull K, Ullu E. Double-stranded RNA induces mRNA degradation in Trypanosoma brucei. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 14687-14692. PMID: 9843950, PMCID: PMC24510, DOI: 10.1073/pnas.95.25.14687.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsMicroscopy, ElectronRNARNA, MessengerTransfectionTrypanosoma brucei bruceiTubulinConceptsMRNA degradationUntranslated regionFlagellar attachment zoneMRNA 5' untranslated regionAlpha-tubulin synthesisImportant protozoan parasitesCaenorhabditis elegansAlpha-tubulin mRNACellular mRNAsTrypanosome cellsGenetic interferenceCytoskeletal structuresCleavage furrowDsRNA transfectionSame phenotypeCytokinesisComplex cortical structureFlagellar axonemeTubulin mRNAPlasmid vectorProtozoan parasiteVivo expressionAttachment zoneMRNADsRNAExonic Sequences in the 5′ Untranslated Region of α-Tubulin mRNA Modulate trans Splicing in Trypanosoma brucei
López-Estraño C, Tschudi C, Ullu E. Exonic Sequences in the 5′ Untranslated Region of α-Tubulin mRNA Modulate trans Splicing in Trypanosoma brucei. Molecular And Cellular Biology 1998, 18: 4620-4628. PMID: 9671472, PMCID: PMC109048, DOI: 10.1128/mcb.18.8.4620.Peer-Reviewed Original ResearchPhysical and transcriptional analysis of the Trypanosoma brucei genome reveals a typical eukaryotic arrangement with close interspersionof RNA polymerase II- and III-transcribed genes
Marchetti M, Tschudi C, Silva E, Ullu E. Physical and transcriptional analysis of the Trypanosoma brucei genome reveals a typical eukaryotic arrangement with close interspersionof RNA polymerase II- and III-transcribed genes. Nucleic Acids Research 1998, 26: 3591-3598. PMID: 9671824, PMCID: PMC147737, DOI: 10.1093/nar/26.15.3591.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceChloramphenicol O-AcetyltransferaseDNA, ProtozoanEukaryotic CellsGene DosageGenome, ProtozoanMolecular Sequence DataPromoter Regions, GeneticProtozoan ProteinsRibonucleoprotein, U2 Small NuclearRibonucleoproteins, Small NuclearRNA Polymerase IIRNA Polymerase IIIRNA, MessengerTranscription, GeneticTrypanosoma brucei bruceiConceptsPol II transcription unitsPol III genesSmall nuclear RNAU3 small nuclear RNACAT gene cassettePol IITranscription unitRNA genesTranscriptional domainsPol IIICAT mRNAU2 small nuclear RNARNA gene lociPol II genesRNA polymerase IIGene cassettesMRNA processing signalsPolymerase IITranscriptional mapTranscriptional analysisCosmid clonesNuclear RNADistinct initiation sitesCAT geneGene locus
1997
Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements
Günzl A, Ullu E, Dörner M, Fragoso S, Hoffmann K, Milner J, Morita Y, Nguu E, Vanacova S, Wünsch S, Dare A, Kwon H, Tschudi C. Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements. Molecular And Biochemical Parasitology 1997, 85: 67-76. PMID: 9108549, DOI: 10.1016/s0166-6851(96)02816-2.Peer-Reviewed Original Research
1995
Accurate Modification of the Trypanosome Spliced Leader Cap Structure in a Homologous Cell-free System (∗)
Ullu E, Tschudi C. Accurate Modification of the Trypanosome Spliced Leader Cap Structure in a Homologous Cell-free System (∗). Journal Of Biological Chemistry 1995, 270: 20365-20369. PMID: 7657609, DOI: 10.1074/jbc.270.35.20365.Peer-Reviewed Original ResearchConceptsSL RNACap structureCell-free systemRibonucleoprotein particleUnusual cap structureSpliced leader sequenceTrans-splicing activityHomologous cell-free systemRNA maturationTrypanosomatid protozoaNucleotide compositionC. fasciculataLeader sequenceTrypanosoma bruceiCell-free extractsT. bruceiT. cruziRNANucleotides 7Vitro reactionBruceiTwo-dimensional thin-layer chromatographyL. amazonensisLeishmania amazonensisCrithidia
1994
Trypanosomatid protozoa provide paradigms of eukaryotic biology.
Tschudi C, Ullu E. Trypanosomatid protozoa provide paradigms of eukaryotic biology. Infectious Agents And Disease 1994, 3: 181-6. PMID: 7827786.Peer-Reviewed Original ResearchA common pyrimidine-rich motif governs trans-splicing and polyadenylation of tubulin polycistronic pre-mRNA in trypanosomes.
Matthews K, Tschudi C, Ullu E. A common pyrimidine-rich motif governs trans-splicing and polyadenylation of tubulin polycistronic pre-mRNA in trypanosomes. Genes & Development 1994, 8: 491-501. PMID: 7907303, DOI: 10.1101/gad.8.4.491.Peer-Reviewed Original Research
1993
2'-O-methyl RNA oligonucleotides identify two functional elements in the trypanosome spliced leader ribonucleoprotein particle
Ullu E, Tschudi C. 2'-O-methyl RNA oligonucleotides identify two functional elements in the trypanosome spliced leader ribonucleoprotein particle. Journal Of Biological Chemistry 1993, 268: 13068-13073. PMID: 8514747, DOI: 10.1016/s0021-9258(19)38620-x.Peer-Reviewed Original ResearchTemporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts.
Ullu E, Matthews K, Tschudi C. Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts. Molecular And Cellular Biology 1993, 13: 720-725. PMID: 8417363, PMCID: PMC358950, DOI: 10.1128/mcb.13.1.720.Peer-Reviewed Original ResearchConceptsRNA-processing reactionsTrans splicingPre-mRNASplice sitePolycistronic transcription unitsSplice acceptor siteTrypanosome genesMRNA biogenesisTranscription unitTrypanosome mRNAsLeader RNATubulin transcriptsSplicingAlpha-tubulinPolyadenylationMRNA synthesisAcceptor sitesTrypanosomesTemporal orderMRNABiogenesisGenesTranscriptsRNAOrganisms
1991
Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA.
Ullu E, Tschudi C. Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1991, 88: 10074-10078. PMID: 1719544, PMCID: PMC52870, DOI: 10.1073/pnas.88.22.10074.Peer-Reviewed Original Research
1990
Permeable trypanosome cells as a model system for transceiption and trans-splicing
Ullu E, Tschudi C. Permeable trypanosome cells as a model system for transceiption and trans-splicing. Nucleic Acids Research 1990, 18: 3319-3326. PMID: 2356121, PMCID: PMC330939, DOI: 10.1093/nar/18.11.3319.Peer-Reviewed Original ResearchConceptsAlpha-tubulin RNASL RNAFree linear moleculesHigh molecular weight RNAMolecular weight RNARNA speciesLeader RNATrypanosome cellsDifferent genesWeight RNARNATranscriptionModel systemCritical rolePermeable cellsConcentrations of monovalentIntronsCellsGenesLesser extentHigh potassium concentrationSpeciesTrypanosomaMgCl2 concentrationLinear molecules
1985
Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.
Tschudi C, Young A, Ruben L, Patton C, Richards F. Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 3998-4002. PMID: 3858856, PMCID: PMC397921, DOI: 10.1073/pnas.82.12.3998.Peer-Reviewed Original Research
1983
Reverse transcription of trypanosome variable antigen mRNAs initiated by a specific oligonucleotide primer.
Merritt S, Tschudi C, Konigsberg W, Richards F. Reverse transcription of trypanosome variable antigen mRNAs initiated by a specific oligonucleotide primer. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 1536-1540. PMID: 6188156, PMCID: PMC393636, DOI: 10.1073/pnas.80.6.1536.Peer-Reviewed Original Research