Featured Publications
A Programmable DNA Origami Platform for Organizing Intrinsically Disordered Nucleoporins within Nanopore Confinement
Fisher PDE, Shen Q, Akpinar B, Davis LK, Chung KKH, Baddeley D, Šarić A, Melia TJ, Hoogenboom BW, Lin C, Lusk CP. A Programmable DNA Origami Platform for Organizing Intrinsically Disordered Nucleoporins within Nanopore Confinement. ACS Nano 2018, 12: 1508-1518. PMID: 29350911, PMCID: PMC5834394, DOI: 10.1021/acsnano.7b08044.Peer-Reviewed Original ResearchConceptsTransport channelsAtomic force microscopyMolecular dynamics simulationsHigh-speed atomic force microscopyDNA origami platformFG domainsNuclear pore complexes (NPCs) formChannel mimicsCentral transport channelNuclear pore proteinsForce microscopyDNA origamiNuclear transport receptorsDynamics simulationsSelective transportNanopore confinementMolecular exchangePermeability propertiesDNA cylindersChemical compositionFG networkPore proteinsPolymer modelTransport receptorsCollective properties
2022
Omicron-specific mRNA vaccination alone and as a heterologous booster against SARS-CoV-2
Fang Z, Peng L, Filler R, Suzuki K, McNamara A, Lin Q, Renauer PA, Yang L, Menasche B, Sanchez A, Ren P, Xiong Q, Strine M, Clark P, Lin C, Ko AI, Grubaugh ND, Wilen CB, Chen S. Omicron-specific mRNA vaccination alone and as a heterologous booster against SARS-CoV-2. Nature Communications 2022, 13: 3250. PMID: 35668119, PMCID: PMC9169595, DOI: 10.1038/s41467-022-30878-4.Peer-Reviewed Original ResearchConceptsHeterologous boosterSARS-CoV-2Antibody responseMRNA vaccinesMRNA vaccinationDelta variantOmicron variantType of vaccinationStrong antibody responseMRNA vaccine candidatesVaccine candidatesNeutralization potencyImmune evasionSARS-CoV.Two weeksComparable titersVaccinationVaccineTiters 10MiceOmicronWeeksWA-1LNP-mRNABoosterVariant-specific vaccination induces systems immune responses and potent in vivo protection against SARS-CoV-2
Peng L, Renauer PA, Ökten A, Fang Z, Park JJ, Zhou X, Lin Q, Dong MB, Filler R, Xiong Q, Clark P, Lin C, Wilen CB, Chen S. Variant-specific vaccination induces systems immune responses and potent in vivo protection against SARS-CoV-2. Cell Reports Medicine 2022, 3: 100634. PMID: 35561673, PMCID: PMC9040489, DOI: 10.1016/j.xcrm.2022.100634.Peer-Reviewed Original ResearchConceptsImmune responseImmune cell populationsSARS-CoV-2 spikeAssessment of efficacySARS-CoV-2LNP-mRNABreakthrough infectionsCD8 TImmune profilingMRNA vaccinesPotent protectionT lymphocytesNeutralization activityDelta variantAnimal modelsPotent antibodiesRepertoire diversityCell responsesAuthentic virusSystemic increaseVariant lineagesClonal expansionCell populationsCOVID-19Vaccination
2020
DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells
Williams ND, Landajuela A, Kasula RK, Zhou W, Powell JT, Xi Z, Isaacs FJ, Berro J, Toomre D, Karatekin E, Lin C. DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells. Nano Letters 2020, 20: 8890-8896. PMID: 33164530, PMCID: PMC7726105, DOI: 10.1021/acs.nanolett.0c03925.Peer-Reviewed Original ResearchConceptsCopies of proteinsBrightness standardsFluorescence microscopyProtein countingMammalian cellsFluorescent proteinCopy numberLive cellsConventional quantification techniquesBiological LaboratoryFluorescence microscopeProteinDNA origamiVersatile toolCellsBiologyCurrent quantification methodsCopiesOrganic dyes
2007
Self-Assembled Combinatorial Encoding Nanoarrays for Multiplexed Biosensing
Lin C, Liu Y, Yan H. Self-Assembled Combinatorial Encoding Nanoarrays for Multiplexed Biosensing. Nano Letters 2007, 7: 507-512. PMID: 17298017, PMCID: PMC1963466, DOI: 10.1021/nl062998n.Peer-Reviewed Original ResearchConceptsDifferent molecular probesMultiplexed biosensingNucleic acid probesSensitive detectionMolecular probesMultiplexed detectionAcid probesDNA hybridizationBinding reactionNucleic acidsFluorescent dyeInterprobe distanceDisease diagnosticsSensor platformMoleculesMultiple DNA sequencesTwo-dimensional arraySmall amountBioconjugationBiosensingNanoarraysAptamerDyeProbeNanotiles