Featured Publications
Submicrometre geometrically encoded fluorescent barcodes self-assembled from DNA
Lin C, Jungmann R, Leifer AM, Li C, Levner D, Church GM, Shih WM, Yin P. Submicrometre geometrically encoded fluorescent barcodes self-assembled from DNA. Nature Chemistry 2012, 4: 832-839. PMID: 23000997, PMCID: PMC3523708, DOI: 10.1038/nchem.1451.Peer-Reviewed Original Research
2022
Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging
Chung KKH, Zhang Z, Kidd P, Zhang Y, Williams ND, Rollins B, Yang Y, Lin C, Baddeley D, Bewersdorf J. Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging. Nature Methods 2022, 19: 554-559. PMID: 35501386, PMCID: PMC9133131, DOI: 10.1038/s41592-022-01464-9.Peer-Reviewed Original ResearchConceptsSlow imaging speedSuper-resolution imagingSuper-resolution microscopy methodsDNA-PAINTOptical sectioningImaging speedFast imagingNanometer resolutionDNA-based points accumulationHigh-fidelity imagesUnbound fluorophoresMicroscopy methodsDocking strandsHigh backgroundPoint accumulationNanoscale topographyProbe
2020
DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells
Williams ND, Landajuela A, Kasula RK, Zhou W, Powell JT, Xi Z, Isaacs FJ, Berro J, Toomre D, Karatekin E, Lin C. DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells. Nano Letters 2020, 20: 8890-8896. PMID: 33164530, PMCID: PMC7726105, DOI: 10.1021/acs.nanolett.0c03925.Peer-Reviewed Original ResearchConceptsCopies of proteinsBrightness standardsFluorescence microscopyProtein countingMammalian cellsFluorescent proteinCopy numberLive cellsConventional quantification techniquesBiological LaboratoryFluorescence microscopeProteinDNA origamiVersatile toolCellsBiologyCurrent quantification methodsCopiesOrganic dyes
2015
Controlled Co-reconstitution of Multiple Membrane Proteins in Lipid Bilayer Nanodiscs Using DNA as a Scaffold
Raschle T, Lin C, Jungmann R, Shih WM, Wagner G. Controlled Co-reconstitution of Multiple Membrane Proteins in Lipid Bilayer Nanodiscs Using DNA as a Scaffold. ACS Chemical Biology 2015, 10: 2448-2454. PMID: 26356202, PMCID: PMC4769731, DOI: 10.1021/acschembio.5b00627.Peer-Reviewed Original ResearchMeSH KeywordsDNAFluorescent DyesIon ChannelsLipid BilayersMembrane ProteinsMicroscopy, FluorescenceModels, BiologicalNanostructuresNuclear MatrixConceptsMembrane protein complexesMultiple membrane proteinsVoltage-gated anion channelsMembrane proteinsProtein complexesHeterotrimeric membrane protein complexIndividual membrane proteinsLipid bilayer nanodiscsSingle nanodiscBilayer nanodiscsProtein aggregationMembrane environmentAnion channelNanodiscsProteinLipid bilayersModel systemProof of principleOligonucleotide sequencesComplementary oligonucleotide sequencesComplexesTemporary attachmentDNASequenceBilayers