Featured Publications
Actuating tension-loaded DNA clamps drives membrane tubulation
Liu L, Xiong Q, Xie C, Pincet F, Lin C. Actuating tension-loaded DNA clamps drives membrane tubulation. Science Advances 2022, 8: eadd1830. PMID: 36223466, PMCID: PMC9555772, DOI: 10.1126/sciadv.add1830.Peer-Reviewed Original ResearchConceptsDNA clampMembrane tubulationMembrane dynamicsMembrane-remodeling eventsVesicle tubulationConformational changesSpatiotemporal controlDNA signalsCell membraneDNA nanostructuresTubulationMembrane deformationClosed stateOpen stateSelf-assembled DNA nanostructuresOrganismsProteinMembrane tubeArtificial systemsTube widthMembraneDynamicsA programmable DNA-origami platform for studying lipid transfer between bilayers
Bian X, Zhang Z, Xiong Q, De Camilli P, Lin C. A programmable DNA-origami platform for studying lipid transfer between bilayers. Nature Chemical Biology 2019, 15: 830-837. PMID: 31320758, PMCID: PMC6650167, DOI: 10.1038/s41589-019-0325-3.Peer-Reviewed Original ResearchConceptsLipid transferNon-vesicular lipid transportSynaptotagmin-like mitochondrial lipid-binding protein (SMP) domainLipid transportMembrane contact sitesLipid transport proteinsSMP domainImportant physiological roleDNA origami platformProtein domainsUnstructured linkerContact sitesSynaptotagmin-1Förster resonance energy transferPhysiological roleResonance energy transferMechanistic insightsDNA origami nanostructuresAcceptor liposomesVesicle Tubulation with Self‐Assembling DNA Nanosprings
Grome MW, Zhang Z, Pincet F, Lin C. Vesicle Tubulation with Self‐Assembling DNA Nanosprings. Angewandte Chemie International Edition 2018, 57: 5330-5334. PMID: 29575478, PMCID: PMC5924453, DOI: 10.1002/anie.201800141.Peer-Reviewed Original ResearchMeSH KeywordsDNALipid BilayersMicroscopy, FluorescenceNanostructuresNanotechnologyNucleic Acid ConformationPolymerizationConceptsMembrane-deforming proteinsDNA origami designMembrane tubulationMembrane tubulesMembrane curvatureMembrane surface coverageVesicle tubulationDNA structureLipid bilayersTubulationNanospringsTube morphologyIntricate interplayArtificial nanomachinesVesicle deformationSpherical vesiclesNanotechnologyMajor goalProteinDNAVesiclesNanomachinesBioengineeringDetergentsMorphologyPlacing and shaping liposomes with reconfigurable DNA nanocages
Zhang Z, Yang Y, Pincet F, Llaguno M, Lin C. Placing and shaping liposomes with reconfigurable DNA nanocages. Nature Chemistry 2017, 9: 653-659. PMID: 28644472, PMCID: PMC5542812, DOI: 10.1038/nchem.2802.Peer-Reviewed Original ResearchConceptsMembrane-bound vesiclesDNA cagesRegulated deformationsDNA nanocagesMembrane curvatureMembrane fusionConformational changesBiological membranesCell membraneLipid bilayer membranesMembrane mechanicsVesiclesDiverse structuresMembraneCellsBilayer membranesVersatile toolDelivery vesiclesToroid shapeLiposome shapeSelf-assembly of size-controlled liposomes on DNA nanotemplates
Yang Y, Wang J, Shigematsu H, Xu W, Shih WM, Rothman JE, Lin C. Self-assembly of size-controlled liposomes on DNA nanotemplates. Nature Chemistry 2016, 8: 476-483. PMID: 27102682, PMCID: PMC5021307, DOI: 10.1038/nchem.2472.Peer-Reviewed Original ResearchConceptsDNA nanotemplatesArtificial lipid bilayer membranesLipid bilayer formationLipid bilayer membranesSelf-AssemblyTemplating methodKey intermediateBilayer formationDrug deliveryArtificial vesiclesLipid compositionNanoscale precisionNanotemplatesHomogeneous liposomesUnilamellar vesiclesLiposome formationVesicular transportProtein structureMembrane structureVesicle sizeLiposomesIntermediatesFormationVesiclesStructurePurification of DNA-origami nanostructures by rate-zonal centrifugation
Lin C, Perrault SD, Kwak M, Graf F, Shih WM. Purification of DNA-origami nanostructures by rate-zonal centrifugation. Nucleic Acids Research 2012, 41: e40-e40. PMID: 23155067, PMCID: PMC3553994, DOI: 10.1093/nar/gks1070.Peer-Reviewed Original ResearchConceptsDNA origami nanostructuresDNA nanostructuresDNA origami structuresAgarose gel electrophoresisDNA origami constructsNanostructuresAqueous solutionFraction collectionSeparation resolutionGradient mixingPurification processHigh yieldsPurification approachConcentration stepRate zonal centrifugationCentrifuge tubeAgarose gelDyeAvailable equipmentDesaltingGelContaminantsPurificationPreparationSeparationIn vivo cloning of artificial DNA nanostructures
Lin C, Rinker S, Wang X, Liu Y, Seeman NC, Yan H. In vivo cloning of artificial DNA nanostructures. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 17626-17631. PMID: 18927233, PMCID: PMC2584761, DOI: 10.1073/pnas.0805416105.Peer-Reviewed Original ResearchMeSH KeywordsCloning, MolecularCross-Linking ReagentsDNADNA ReplicationFicusinNanostructuresNucleic Acid ConformationConceptsArtificial DNA nanostructuresDNA nanostructuresDNA nanotechnologyGenetic information carriersStandard molecular biology techniquesNanostructuresVersatile methodFour-arm junctionsVivo cloningMolecular biology techniquesNumber of assaysComplex secondary structureBiology techniquesNanotechnologyHigh copy numberLiving cellsFerguson analysisHelper phageCorrect replicationPossible solutionsSecondary structureCopy numberPhagemidCarriersDNA
2023
Recent Advances in DNA Origami-Engineered Nanomaterials and Applications
Zhan P, Peil A, Jiang Q, Wang D, Mousavi S, Xiong Q, Shen Q, Shang Y, Ding B, Lin C, Ke Y, Liu N. Recent Advances in DNA Origami-Engineered Nanomaterials and Applications. Chemical Reviews 2023, 123: 3976-4050. PMID: 36990451, PMCID: PMC10103138, DOI: 10.1021/acs.chemrev.3c00028.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsDNA origami techniquePaul RothemundDNA nanotechnologyOrigami techniqueExciting achievementsNanomaterialsMaterials scienceRecent progressRecent advancesNanotechnologyUseful applicationsApplicationsRothemundSignificant advancesUnexplored research avenuesOriginal proposalFieldPhysicsMathematicsEngineeringAdvancesUnique field
2022
Frame-Guided Assembly of Amphiphiles
Dong Y, Yang Y, Lin C, Liu D. Frame-Guided Assembly of Amphiphiles. Accounts Of Chemical Research 2022, 55: 1938-1948. PMID: 35786832, DOI: 10.1021/acs.accounts.2c00234.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsHydrophobic and Hydrophilic InteractionsLiposomesMembrane ProteinsNanostructuresProspective StudiesConceptsFrame-guided assemblyMolecular structureAssembly of amphiphilesArrangement of moleculesTraditional chemical industryMembrane protein incorporationProtein-lipid bilayerAmphiphilic assembliesAssembly morphologyAmphiphile assembliesFunctional nanomaterialsVersatile strategyAqueous environmentHydrophobic groupsHydrophobic moleculesFull surface areaHydrophobic interactionsAmphiphilesDrug deliveryMembrane proteinsFunctional complexAmphiphile concentrationAssembly strategyEPR effectNanomaterials
2020
RNA returns to the fold
Shen Q, Lin C. RNA returns to the fold. Nature Chemistry 2020, 12: 221-222. PMID: 32108762, DOI: 10.1038/s41557-020-0432-5.Commentaries, Editorials and LettersNanostructuresRNA
2019
Engineering Lipid Membranes with Programmable DNA Nanostructures
Shen Q, Grome MW, Yang Y, Lin C. Engineering Lipid Membranes with Programmable DNA Nanostructures. Advanced Biology 2019, 4 PMID: 31934608, PMCID: PMC6957268, DOI: 10.1002/adbi.201900215.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsDNA nanostructuresProgrammable DNA nanostructuresGenetic informationLipid membranesCell's genetic informationAmphipathic lipid moleculesLipid/DNA complexesSelf-assembling capabilitiesDNA-based toolsQuantitative biophysical studiesNanoscopic precisionProgrammable nanostructuresControllable structureHybrid materialsChromatin structureGene therapyNanostructuresDistant moleculesSynthetic biologyAbundant biomoleculesLipid moleculesBiophysical studiesDNA complexesExcellent materialCell nucleiStiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation
Grome MW, Zhang Z, Lin C. Stiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation. ACS Applied Materials & Interfaces 2019, 11: 22987-22992. PMID: 31252462, PMCID: PMC6613048, DOI: 10.1021/acsami.9b05401.Peer-Reviewed Original ResearchConceptsVesicle tubulationMembrane-deforming proteinsDNA-based constructsMembrane anchorArtificial assemblageMembrane bindingSubcellular membranesDNA nanostructuresMembrane affinityTubulationLipid tubulesMembraneDNA nanotechnologyTunable architecturesPeptide densitySurface of liposomesProteinNanostructuresAssemblagesBindingAffinity
2016
DNA Origami Rotaxanes: Tailored Synthesis and Controlled Structure Switching
Powell JT, Akhuetie‐Oni B, Zhang Z, Lin C. DNA Origami Rotaxanes: Tailored Synthesis and Controlled Structure Switching. Angewandte Chemie International Edition 2016, 55: 11412-11416. PMID: 27527591, PMCID: PMC5019031, DOI: 10.1002/anie.201604621.Peer-Reviewed Original ResearchMeSH KeywordsDNAMacrocyclic CompoundsMicroscopy, Electron, TransmissionNanostructuresNucleic Acid HybridizationRotaxanesConceptsRotaxane assemblySupramolecular assembliesAssembly routeStructure switchingStructural switchingRotaxanesFunctional nanodevicesUnique structureBuilding blocksMacrocyclesDNA hybridizationElectron microscopyFinal productMultistep assemblyAssemblySynthesisStructural integrityHereinNanodevicesTranslational motionMicroscopySecond mechanismFirst mechanismRouteElectrophoresis
2015
Controlled Co-reconstitution of Multiple Membrane Proteins in Lipid Bilayer Nanodiscs Using DNA as a Scaffold
Raschle T, Lin C, Jungmann R, Shih WM, Wagner G. Controlled Co-reconstitution of Multiple Membrane Proteins in Lipid Bilayer Nanodiscs Using DNA as a Scaffold. ACS Chemical Biology 2015, 10: 2448-2454. PMID: 26356202, PMCID: PMC4769731, DOI: 10.1021/acschembio.5b00627.Peer-Reviewed Original ResearchConceptsMembrane protein complexesMultiple membrane proteinsVoltage-gated anion channelsMembrane proteinsProtein complexesHeterotrimeric membrane protein complexIndividual membrane proteinsLipid bilayer nanodiscsSingle nanodiscBilayer nanodiscsProtein aggregationMembrane environmentAnion channelNanodiscsProteinLipid bilayersModel systemProof of principleOligonucleotide sequencesComplementary oligonucleotide sequencesComplexesTemporary attachmentDNASequenceBilayers
2011
Synthesis and Characterization of Self-Assembled DNA Nanostructures
Lin C, Ke Y, Chhabra R, Sharma J, Liu Y, Yan H. Synthesis and Characterization of Self-Assembled DNA Nanostructures. Methods In Molecular Biology 2011, 749: 1-11. PMID: 21674361, DOI: 10.1007/978-1-61779-142-0_1.ChaptersConceptsDNA nanostructuresDNA nanotechnologySelf-assembled DNA nanostructuresSuch nanoscale objectsStructural DNA nanotechnologyCharacterization of SelfDNA-based nanodevicesExcellent yieldsNanoscale objectsNanostructuresArtificial nanostructuresMolecular speciesBuilding blocksNanotechnologyFast evolvementMain building blocksNanoarchitecturesLarge varietySynthesisMaterialsNanodevicesPreparationCharacterizationBuilding materialsYieldRecovery of intact DNA nanostructures after agarose gel–based separation
Bellot G, McClintock MA, Lin C, Shih WM. Recovery of intact DNA nanostructures after agarose gel–based separation. Nature Methods 2011, 8: 192-194. PMID: 21358621, PMCID: PMC3843644, DOI: 10.1038/nmeth0311-192.Peer-Reviewed Original Research
2010
Knitting complex weaves with DNA origami
Shih WM, Lin C. Knitting complex weaves with DNA origami. Current Opinion In Structural Biology 2010, 20: 276-282. PMID: 20456942, PMCID: PMC2916953, DOI: 10.1016/j.sbi.2010.03.009.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsPhotonic interaction between quantum dots and gold nanoparticles in discrete nanostructures through DNA directed self-assembly
Wang Q, Wang H, Lin C, Sharma J, Zou S, Liu Y. Photonic interaction between quantum dots and gold nanoparticles in discrete nanostructures through DNA directed self-assembly. Chemical Communications 2010, 46: 240-242. PMID: 20024338, DOI: 10.1039/b915712c.Peer-Reviewed Original Research
2009
A Replicable Tetrahedral Nanostructure Self-Assembled from a Single DNA Strand
Li Z, Wei B, Nangreave J, Lin C, Liu Y, Mi Y, Yan H. A Replicable Tetrahedral Nanostructure Self-Assembled from a Single DNA Strand. Journal Of The American Chemical Society 2009, 131: 13093-13098. PMID: 19737020, PMCID: PMC3083857, DOI: 10.1021/ja903768f.Peer-Reviewed Original ResearchConceptsAtomic force microscopy imagingForce microscopy imagingRolling-circle amplificationDNA nanostructuresSingle DNA strandsFerguson analysisMicroscopy imagingTetrahedraStandard molecular cloning techniquesDNA strandsSingle strandsRestriction enzyme digestionCloning techniquesMolecular cloning techniquesA cascade of activity
Lin C, Yan H. A cascade of activity. Nature Nanotechnology 2009, 4: 211-212. PMID: 19350024, DOI: 10.1038/nnano.2009.66.Commentaries, Editorials and Letters