Featured Publications
Purification of DNA-origami nanostructures by rate-zonal centrifugation
Lin C, Perrault SD, Kwak M, Graf F, Shih WM. Purification of DNA-origami nanostructures by rate-zonal centrifugation. Nucleic Acids Research 2012, 41: e40-e40. PMID: 23155067, PMCID: PMC3553994, DOI: 10.1093/nar/gks1070.Peer-Reviewed Original ResearchConceptsDNA origami nanostructuresDNA nanostructuresDNA origami structuresAgarose gel electrophoresisDNA origami constructsNanostructuresAqueous solutionFraction collectionSeparation resolutionGradient mixingPurification processHigh yieldsPurification approachConcentration stepRate zonal centrifugationCentrifuge tubeAgarose gelDyeAvailable equipmentDesaltingGelContaminantsPurificationPreparationSeparationIn vivo cloning of artificial DNA nanostructures
Lin C, Rinker S, Wang X, Liu Y, Seeman NC, Yan H. In vivo cloning of artificial DNA nanostructures. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 17626-17631. PMID: 18927233, PMCID: PMC2584761, DOI: 10.1073/pnas.0805416105.Peer-Reviewed Original ResearchConceptsArtificial DNA nanostructuresDNA nanostructuresDNA nanotechnologyGenetic information carriersStandard molecular biology techniquesNanostructuresVersatile methodFour-arm junctionsVivo cloningMolecular biology techniquesNumber of assaysComplex secondary structureBiology techniquesNanotechnologyHigh copy numberLiving cellsFerguson analysisHelper phageCorrect replicationPossible solutionsSecondary structureCopy numberPhagemidCarriersDNA
2021
DNA origami
Dey S, Fan C, Gothelf K, Li J, Lin C, Liu L, Liu N, Nijenhuis M, Saccà B, Simmel F, Yan H, Zhan P. DNA origami. Nature Reviews Methods Primers 2021, 1: 13. DOI: 10.1038/s43586-020-00009-8.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsDNA origami technologyOrigami technologyDNA origami techniqueNear-atomic precisionDNA nanotechnologyOrigami techniqueDNA origamiDrug deliveryOrigami structuresMolecular machinesOrigami designBiological materialsLiving cellsSynthesisVivo synthesisNanophotonicsNanotechnologyNanofabricationNanostructuresNanoelectronicsBioimagingScale of productionFabricationTechnologyFunctionalization
2020
DNA Origami Post‐Processing by CRISPR‐Cas12a
Xiong Q, Xie C, Zhang Z, Liu L, Powell JT, Shen Q, Lin C. DNA Origami Post‐Processing by CRISPR‐Cas12a. Angewandte Chemie International Edition 2020, 59: 3956-3960. PMID: 31883145, PMCID: PMC7101258, DOI: 10.1002/anie.201915555.Peer-Reviewed Original ResearchConceptsDNA origami structuresCRISPR-Cas12aDNA origami devicesDNA origami designDNA origami techniqueNanomaterial fabricationFunctional nanodevicesDNA origamiScaffold strandTremendous promiseCutting-edge toolsVersatile toolNanodevicesNanostructuresFacileFabricationBiocompatibilityBiotechnologySequence specificityOrigamiEnzymatic methodMechanical propertiesStructural transformationDevicesIntricate structure
2019
Engineering Lipid Membranes with Programmable DNA Nanostructures
Shen Q, Grome MW, Yang Y, Lin C. Engineering Lipid Membranes with Programmable DNA Nanostructures. Advanced Biology 2019, 4 PMID: 31934608, PMCID: PMC6957268, DOI: 10.1002/adbi.201900215.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsDNA nanostructuresProgrammable DNA nanostructuresGenetic informationLipid membranesCell's genetic informationAmphipathic lipid moleculesLipid/DNA complexesSelf-assembling capabilitiesDNA-based toolsQuantitative biophysical studiesNanoscopic precisionProgrammable nanostructuresControllable structureHybrid materialsChromatin structureGene therapyNanostructuresDistant moleculesSynthetic biologyAbundant biomoleculesLipid moleculesBiophysical studiesDNA complexesExcellent materialCell nucleiStiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation
Grome MW, Zhang Z, Lin C. Stiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation. ACS Applied Materials & Interfaces 2019, 11: 22987-22992. PMID: 31252462, PMCID: PMC6613048, DOI: 10.1021/acsami.9b05401.Peer-Reviewed Original ResearchConceptsVesicle tubulationMembrane-deforming proteinsDNA-based constructsMembrane anchorArtificial assemblageMembrane bindingSubcellular membranesDNA nanostructuresMembrane affinityTubulationLipid tubulesMembraneDNA nanotechnologyTunable architecturesPeptide densitySurface of liposomesProteinNanostructuresAssemblagesBindingAffinity
2018
Modulation of the Cellular Uptake of DNA Origami through Control over Mass and Shape
Bastings MMC, Anastassacos FM, Ponnuswamy N, Leifer FG, Cuneo G, Lin C, Ingber DE, Ryu JH, Shih WM. Modulation of the Cellular Uptake of DNA Origami through Control over Mass and Shape. Nano Letters 2018, 18: 3557-3564. PMID: 29756442, DOI: 10.1021/acs.nanolett.8b00660.Peer-Reviewed Original ResearchConceptsDNA nanotechnologyStructural DNA nanotechnologyThree-dimensional nanostructuresCellular uptakeDNA origami shapesDesigner nanoparticlesNanoparticle uptakeDNA origamiCell delivery vehicleTherapeutic deliveryAttractive platformCell uptakeNanotechnologyNanoparticlesGreater compactnessDifferent cell linesUnparalleled abilityNanostructuresResolution featuresLarge particlesLimited controlParticle shapeOrigamiRatio particlesParticles
2011
Synthesis and Characterization of Self-Assembled DNA Nanostructures
Lin C, Ke Y, Chhabra R, Sharma J, Liu Y, Yan H. Synthesis and Characterization of Self-Assembled DNA Nanostructures. Methods In Molecular Biology 2011, 749: 1-11. PMID: 21674361, DOI: 10.1007/978-1-61779-142-0_1.ChaptersConceptsDNA nanostructuresDNA nanotechnologySelf-assembled DNA nanostructuresSuch nanoscale objectsStructural DNA nanotechnologyCharacterization of SelfDNA-based nanodevicesExcellent yieldsNanoscale objectsNanostructuresArtificial nanostructuresMolecular speciesBuilding blocksNanotechnologyFast evolvementMain building blocksNanoarchitecturesLarge varietySynthesisMaterialsNanodevicesPreparationCharacterizationBuilding materialsYield
2010
Photonic interaction between quantum dots and gold nanoparticles in discrete nanostructures through DNA directed self-assembly
Wang Q, Wang H, Lin C, Sharma J, Zou S, Liu Y. Photonic interaction between quantum dots and gold nanoparticles in discrete nanostructures through DNA directed self-assembly. Chemical Communications 2010, 46: 240-242. PMID: 20024338, DOI: 10.1039/b915712c.Peer-Reviewed Original Research
2009
Designer DNA Nanoarchitectures
Lin C, Liu Y, Yan H. Designer DNA Nanoarchitectures. Biochemistry 2009, 48: 1663-1674. PMID: 19199428, PMCID: PMC2765550, DOI: 10.1021/bi802324w.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsStructural DNA nanotechnologyDNA nanotechnologyDNA nanoarchitecturesSelf-assembled architecturesWatson-Crick base pairing rulesProtein-protein interactionsBase pairing rulesDNA nanostructuresMulticomponent nanomachinesPrinciples of DNAArtificial nanostructuresNanometer precisionHelical DNA structureUnicellular diatomsFunctional groupsDNA structureNanotechnologyNanostructuresMolecular speciesNanoarchitecturesSuch eleganceExciting progressHigh yieldsBiological systemsDNA
2008
Mirror Image DNA Nanostructures for Chiral Supramolecular Assemblies
Lin C, Ke Y, Li Z, Wang JH, Liu Y, Yan H. Mirror Image DNA Nanostructures for Chiral Supramolecular Assemblies. Nano Letters 2008, 9: 433-436. PMID: 19063615, PMCID: PMC2669104, DOI: 10.1021/nl803328v.Peer-Reviewed Original ResearchConceptsChiral supramolecular assembliesStructural DNA nanotechnologyNatural d-DNACircular dichroism spectraSupramolecular assembliesDNA supramoleculesAtomic force microscopeDNA nanostructuresDNA nanotechnologyL-DNADichroism spectraVivo medical applicationsNuclease resistanceForce microscopePolyacrylamide gel electrophoresisOpposite chiralityNanostructuresD-DNAPeriodic nanostructuresMedical applicationsSupramoleculesGel electrophoresisSubstantial complementChiralityNanotechnology
2006
DNA Tile Based Self‐Assembly: Building Complex Nanoarchitectures
Lin C, Liu Y, Rinker S, Yan H. DNA Tile Based Self‐Assembly: Building Complex Nanoarchitectures. ChemPhysChem 2006, 7: 1641-1647. PMID: 16832805, DOI: 10.1002/cphc.200600260.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsDNA tilesSelf-assembled DNA nanostructuresNanometer-scale materialsNanometer-scale componentsComplex nanoarchitecturesDNA nanostructuresNanophotonic devicesAttractive routeExcellent scaffoldProgrammable patternsNanoarchitecturesPotential applicationsExciting progressNanoarraysNanoparticlesNanostructuresRecent developmentsScaffoldsDevicesRouteMaterials
2005
Aptamer‐Directed Self‐Assembly of Protein Arrays on a DNA Nanostructure
Liu Y, Lin C, Li H, Yan H. Aptamer‐Directed Self‐Assembly of Protein Arrays on a DNA Nanostructure. Angewandte Chemie International Edition 2005, 44: 4333-4338. PMID: 15945116, DOI: 10.1002/anie.200501089.Peer-Reviewed Original Research