2024
Noncanonical interaction with microtubules via the N-terminal nonmotor domain is critical for the functions of a bidirectional kinesin
Singh S, Siegler N, Pandey H, Yanir N, Popov M, Goldstein-Levitin A, Sadan M, Debs G, Zarivach R, Frank G, Kass I, Sindelar C, Zalk R, Gheber L. Noncanonical interaction with microtubules via the N-terminal nonmotor domain is critical for the functions of a bidirectional kinesin. Science Advances 2024, 10: eadi1367. PMID: 38324691, PMCID: PMC10849588, DOI: 10.1126/sciadv.adi1367.Peer-Reviewed Original ResearchMeSH KeywordsCryoelectron MicroscopyKinesinsMicrotubulesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsConceptsBidirectional motilityKinesin-5Plus-end-directed motilityKinesin-5 motorsCryo-EMC-terminal tailCryo-EM mapsPlus-end-directed kinesinCryo-electron microscopyDeletion mutantsNoncanonical interactionsIntracellular functionsCin8Cryo-electronMotor domainMotilityNonmotor domainsMutantsB-tubulinSingle-moleculeCell viabilityIn vivoIn vitroIn vitro experimentsLocal defects
2020
Dynamic and asymmetric fluctuations in the microtubule wall captured by high-resolution cryoelectron microscopy
Debs GE, Cha M, Liu X, Huehn AR, Sindelar CV. Dynamic and asymmetric fluctuations in the microtubule wall captured by high-resolution cryoelectron microscopy. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 16976-16984. PMID: 32636254, PMCID: PMC7382274, DOI: 10.1073/pnas.2001546117.Peer-Reviewed Original Research
2017
Structural basis of cooperativity in kinesin revealed by 3D reconstruction of a two-head-bound state on microtubules
Liu D, Liu X, Shang Z, Sindelar CV. Structural basis of cooperativity in kinesin revealed by 3D reconstruction of a two-head-bound state on microtubules. ELife 2017, 6: e24490. PMID: 28504639, PMCID: PMC5459574, DOI: 10.7554/elife.24490.Peer-Reviewed Original Research
2014
High-resolution structures of kinesin on microtubules provide a basis for nucleotide-gated force-generation
Shang Z, Zhou K, Xu C, Csencsits R, Cochran JC, Sindelar CV. High-resolution structures of kinesin on microtubules provide a basis for nucleotide-gated force-generation. ELife 2014, 3: e04686. PMID: 25415053, PMCID: PMC4383081, DOI: 10.7554/elife.04686.Peer-Reviewed Original Research
2007
The beginning of kinesin's force-generating cycle visualized at 9-Å resolution
Sindelar CV, Downing KH. The beginning of kinesin's force-generating cycle visualized at 9-Å resolution. Journal Of Cell Biology 2007, 177: 377-385. PMID: 17470637, PMCID: PMC2064809, DOI: 10.1083/jcb.200612090.Peer-Reviewed Original ResearchConceptsSwitch II helixMicrotubule-binding proteinN-terminal extensionII helixNucleotide-free stateCryo-electron microscopySwitch IMicrotubule contactsResponse elementSingle-particle reconstructionConformational changesMicrotubulesActivation mechanismKinesinHigh-resolution characterizationHelixProtein
2003
Thermodynamic Properties of the Kinesin Neck-Region Docking to the Catalytic Core
Rice S, Cui Y, Sindelar C, Naber N, Matuska M, Vale R, Cooke R. Thermodynamic Properties of the Kinesin Neck-Region Docking to the Catalytic Core. Biophysical Journal 2003, 84: 1844-1854. PMID: 12609886, PMCID: PMC1302753, DOI: 10.1016/s0006-3495(03)74992-3.Peer-Reviewed Original Research