2024
Selective utilization of glucose metabolism guides mammalian gastrulation
Cao D, Bergmann J, Zhong L, Hemalatha A, Dingare C, Jensen T, Cox A, Greco V, Steventon B, Sozen B. Selective utilization of glucose metabolism guides mammalian gastrulation. Nature 2024, 634: 919-928. PMID: 39415005, PMCID: PMC11499262, DOI: 10.1038/s41586-024-08044-1.Peer-Reviewed Original ResearchConceptsCellular metabolismMammalian gastrulationHexosamine biosynthetic pathwayTranscription factor networksCellular signaling pathwaysSignaling morphogensGlucose metabolismCellular programmeBiosynthetic pathwayFate acquisitionCell fateHousekeeping natureGenetic mechanismsMesoderm migrationFactor networksERK activationExpression patternsSignaling pathwayDevelopmental processesStem cell modelCell typesSpecialized functionsDevelopmental contextMammalian embryosMouse embryos
2023
Self-patterning of human stem cells into post-implantation lineages
Pedroza M, Gassaloglu S, Dias N, Zhong L, Hou T, Kretzmer H, Smith Z, Sozen B. Self-patterning of human stem cells into post-implantation lineages. Nature 2023, 622: 574-583. PMID: 37369348, PMCID: PMC10584676, DOI: 10.1038/s41586-023-06354-4.Peer-Reviewed Original ResearchConceptsStem cellsPlacental cell typesPost-implantation embryonic developmentHuman pluripotent stem cellsPluripotent stem cellsHuman embryonic developmentEmbryonic developmentHuman stem cellsCongenital pathologyPost-implantation epiblastDiverse cell statesSingle-cell transcriptomicsAmniotic ectodermExtra-embryonic endodermSpontaneous differentiationSignaling hubThree-dimensional structureSecreted modulatorsCell types
2019
Self-Assembly of Embryonic and Two Extraembryonic Stem Cell Types Into Gastrulating Embryo-like Structures
Sozen B, Amadei G, Cox A, Wang R, Na E, Czukiewska S, Chappell L, Voet T, Michel G, Jing N, Glover D, Zernicka-Goetz M. Self-Assembly of Embryonic and Two Extraembryonic Stem Cell Types Into Gastrulating Embryo-like Structures. Obstetrical & Gynecological Survey 2019, 74: 30-31. DOI: 10.1097/01.ogx.0000553115.73937.07.Peer-Reviewed Original Research
2018
Self-assembly of embryonic and two extra-embryonic stem cell types into gastrulating embryo-like structures
Sozen B, Amadei G, Cox A, Wang R, Na E, Czukiewska S, Chappell L, Voet T, Michel G, Jing N, Glover D, Zernicka-Goetz M. Self-assembly of embryonic and two extra-embryonic stem cell types into gastrulating embryo-like structures. Nature Cell Biology 2018, 20: 979-989. PMID: 30038254, DOI: 10.1038/s41556-018-0147-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CommunicationCell DifferentiationCell LineCell LineageCell MovementCoculture TechniquesEmbryo, MammalianEndodermEpithelial-Mesenchymal TransitionGastrulationGene Expression Regulation, DevelopmentalGestational AgeMiceMouse Embryonic Stem CellsPhenotypeTranscriptomeTrophoblastsConceptsStem cell typesEpithelial-mesenchymal transitionStem cellsExtra-embryonic compartmentCell typesTrophoblast stem cellsEndoderm stem cellsEmbryonic stem cellsMammalian embryosSpatial transcriptomic analysisTrophoblastGene expression patternsGerm lineMiceGastrulating embryosEmbryo-like structuresIn vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells
Harrison S, Sozen B, Zernicka-Goetz M. In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells. Nature Protocols 2018, 13: 1586-1602. PMID: 29988106, DOI: 10.1038/s41596-018-0005-x.Peer-Reviewed Original ResearchConceptsTrophoblast stem cellsEmbryonic stem cellsMouse embryonic stem cellsExtra-embryonic tissuesStem cellsGerm-layer specificationEmbryos in vitroExtracellular matrixStem cell typesIn vitro generationPost-implantation developmentEmbryo-like structuresEarly embryos in vitroBody planMammalian developmentMammalian embryogenesisTissue in vitroEarly embryosEmbryonic tissues in vitroAggregates of embryonic stem cellsCell numberCo-cultureEmbryonic architectureCell typesCulture conditions
2017
Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro
Harrison S, Sozen B, Christodoulou N, Kyprianou C, Zernicka-Goetz M. Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro. Science 2017, 356 PMID: 28254784, DOI: 10.1126/science.aal1810.Peer-Reviewed Original ResearchConceptsTrophoblast stem cellsStem cellsEmbryonic stem cellsGenetically modified stem cellsExtraembryonic stem cellsGerm cell markersPrimordial germ cell markersStem cell typesCell typesExpression of mesodermCell markersNatural embryoDevelopmental potentialExtraembryonic tissuesMouse embryonic stem cellsMammalian embryogenesisBMP signalingResponse to WntCellsCross-talkThree-dimensional scaffoldsEmbryosSelf-assembly in vitroCoordinated morphogenesis
2015
CD90 and CD105 expression in the mouse ovary and testis at different stages of postnatal development
Tepekoy F, Ozturk S, Sozen B, Ozay R, Akkoyunlu G, Demir N. CD90 and CD105 expression in the mouse ovary and testis at different stages of postnatal development. Reproductive Biology 2015, 15: 195-204. PMID: 26679159, DOI: 10.1016/j.repbio.2015.10.004.Peer-Reviewed Original ResearchConceptsExpression of CD90Postnatal developmentGerm cellsPostnatal dayCell typesSomatic cellsGerm cell compartmentMesenchymal stem cell markersStem cell markersStages of gonadal developmentStages of postnatal developmentExpression of CD105Female gonadal cellsMouse gonadsSertoli cellsPostnatal gonadsTesticular expressionMouse ovariesGonadal cellsCD105 expressionCell markersGonadal developmentAdult ovariesImmunohistochemical localizationCD105