Featured Publications
The History of Engineered Tracheal Replacements: Interpreting the Past and Guiding the Future
Greaney A, Niklason L. The History of Engineered Tracheal Replacements: Interpreting the Past and Guiding the Future. Tissue Engineering Part B Reviews 2020, 27: 341-352. PMID: 33045942, PMCID: PMC8390779, DOI: 10.1089/ten.teb.2020.0238.Peer-Reviewed Original ResearchConceptsFailure modesTracheal graftsTracheal replacementGraft mechanicsDesign criteriaInert materialLong segment defectType of graftRecent preclinical workClinical care prioritiesRigid tubeLeuven protocolTracheal allograftsClinical followAirway reconstructionCadaveric allograftsCircumferential defectsTissue analysis techniquesEngineersPreclinical workReplacement graftsCare prioritiesGraft performanceHuman patientsGraftPlatform Effects on Regeneration by Pulmonary Basal Cells as Evaluated by Single-Cell RNA Sequencing
Greaney AM, Adams TS, Raredon M, Gubbins E, Schupp JC, Engler AJ, Ghaedi M, Yuan Y, Kaminski N, Niklason LE. Platform Effects on Regeneration by Pulmonary Basal Cells as Evaluated by Single-Cell RNA Sequencing. Cell Reports 2020, 30: 4250-4265.e6. PMID: 32209482, PMCID: PMC7175071, DOI: 10.1016/j.celrep.2020.03.004.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingBasal marker expressionBasal cellsChronic pulmonary diseaseRat tracheal epitheliumPulmonary diseaseRNA sequencingCell-based therapiesRat tracheaAir-liquid interfaceTissue graftMarker expressionTracheal epitheliumRegenerative outcomesTracheaEpithelial progenitorsDifferential outcomesEpitheliumOutcomesWhole organPopulation level
2016
Engineered Tissue–Stent Biocomposites as Tracheal Replacements
Zhao L, Sundaram S, Le AV, Huang AH, Zhang J, Hatachi G, Beloiartsev A, Caty MG, Yi T, Leiby K, Gard A, Kural MH, Gui L, Rocco KA, Sivarapatna A, Calle E, Greaney A, Urbani L, Maghsoudlou P, Burns A, DeCoppi P, Niklason LE. Engineered Tissue–Stent Biocomposites as Tracheal Replacements. Tissue Engineering Part A 2016, 22: 1086-1097. PMID: 27520928, PMCID: PMC5312617, DOI: 10.1089/ten.tea.2016.0132.Peer-Reviewed Original ResearchElastic, silk‐cardiac extracellular matrix hydrogels exhibit time‐dependent stiffening that modulates cardiac fibroblast response
Stoppel W, Gao A, Greaney A, Partlow B, Bretherton R, Kaplan D, Black L. Elastic, silk‐cardiac extracellular matrix hydrogels exhibit time‐dependent stiffening that modulates cardiac fibroblast response. Journal Of Biomedical Materials Research Part A 2016, 104: 3058-3072. PMID: 27480328, PMCID: PMC5805141, DOI: 10.1002/jbm.a.35850.Peer-Reviewed Original ResearchConceptsExtracellular matrix hydrogelTissue-derived extracellular matrixEndothelial cell ingrowthSilk hydrogelsMechanical propertiesCell ingrowthWeeks in vivoHydrogel stiffeningMatrix hydrogelHydrogelsFunctional cardiac repairStiffeningFocal adhesion proteinsCardiac fibroblastsExpression of integrinsCardiac repairHeart failureProgression to heart failureTissue in vitroAdhesion proteinsCell growthExtracellular matrix