2016
CD301b+ dendritic cells stimulate tissue-resident memory CD8+ T cells to protect against genital HSV-2
Shin H, Kumamoto Y, Gopinath S, Iwasaki A. CD301b+ dendritic cells stimulate tissue-resident memory CD8+ T cells to protect against genital HSV-2. Nature Communications 2016, 7: 13346. PMID: 27827367, PMCID: PMC5105190, DOI: 10.1038/ncomms13346.Peer-Reviewed Original ResearchConceptsCD8 TRM cellsAntigen-presenting cellsGenital HSV-2 infectionTissue-resident memory CD8HSV-2 infectionTRM cellsDendritic cellsMemory CD8Protective immunityT cellsPopulations of APCsGenital HSV-2Protective immune responseFemale genital tractMHC class IHSV-2Genital tractAntigen presentationImmune responseLamina propriaViral infectionConfer protectionBarrier tissuesClass IInfection
2014
Application of the Proximity-Dependent Assay and Fluorescence Imaging Approaches to Study Viral Entry Pathways
Lipovsky A, Zhang W, Iwasaki A, DiMaio D. Application of the Proximity-Dependent Assay and Fluorescence Imaging Approaches to Study Viral Entry Pathways. Methods In Molecular Biology 2014, 1270: 437-451. PMID: 25702134, DOI: 10.1007/978-1-4939-2309-0_30.Peer-Reviewed Original ResearchConceptsGenome-wide RNA interference screenEnriched gene categoriesConfirmation of phenotypesGenome-wide lossRNA interference screenVirus entryFunction genetic screenRNA interference studiesFluorescence imaging approachesProximity ligation assayGenetic screenGene categoriesInterference screenMembrane compartmentsIndividual genesBiochemical approachesCellular compartmentsMolecular intricaciesMicroscopy-based methodBioinformatics analysisProtein locationRNA interferenceCellular organellesViral entry pathwayCellular factors