2022
APOBEC3A regulates transcription from interferon-stimulated response elements
Taura M, Frank JA, Takahashi T, Kong Y, Kudo E, Song E, Tokuyama M, Iwasaki A. APOBEC3A regulates transcription from interferon-stimulated response elements. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2011665119. PMID: 35549556, PMCID: PMC9171812, DOI: 10.1073/pnas.2011665119.Peer-Reviewed Original ResearchConceptsGene expressionResponse elementHost genomic lociInterferon-stimulated response elementRNA sequence analysisLong terminal repeatNegative feedback loopGenomic lociHuman genomeLethal mutationsProximal promoterHIV-1 transcriptionUnexpected roleIFN-I treatmentTerminal repeatDependent inductionViral genomeCytidine deaminaseISG15 inductionAntiviral responseA3AGenomeISG15 expressionType I IFNTranscription
2019
Application of a Modified Smart-seq2 Sample Preparation Protocol for Rare Cell Full-length Single-cell mRNA Sequencing to Mouse Oocytes.
Treger RS, Pope SD, Xing X, Iwasaki A. Application of a Modified Smart-seq2 Sample Preparation Protocol for Rare Cell Full-length Single-cell mRNA Sequencing to Mouse Oocytes. Bio-protocol 2019, 9: e3345. PMID: 33654848, PMCID: PMC7854224, DOI: 10.21769/bioprotoc.3345.Peer-Reviewed Original ResearchEndogenous retrovirusesFull-length cDNA libraryRNA sequencing librariesSmart-seq2 protocolSingle-cell mRNALibrary preparation methodsMurine genomeCDNA librarySequencing librariesExpression analysisERV expressionPrecise regulationGerm cellsIsolate populationMouse oocytesSubsequent sequencingOocytesMature oocytesSample preparation protocolFamily membersRetroelementsGenomeEmbryogenesisLibraryPreparation protocol
2004
Recognition of single-stranded RNA viruses by Toll-like receptor 7
Lund JM, Alexopoulou L, Sato A, Karow M, Adams NC, Gale NW, Iwasaki A, Flavell RA. Recognition of single-stranded RNA viruses by Toll-like receptor 7. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 5598-5603. PMID: 15034168, PMCID: PMC397437, DOI: 10.1073/pnas.0400937101.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsAntigens, DifferentiationBone Marrow CellsChick EmbryoChloroquineCytokinesDendritic CellsEndosomesInterferon-alphaMacrophagesMembrane GlycoproteinsMiceMice, KnockoutMyeloid Differentiation Factor 88OrthomyxoviridaePeritoneumReceptors, Cell SurfaceReceptors, ImmunologicRhabdoviridae InfectionsRNA, ViralSpleenToll-Like Receptor 7Vesicular stomatitis Indiana virusConceptsVesicular stomatitis virusRNA virusesHigh CpG contentGenomes of virusesToll-like receptorsStomatitis virusMammalian genomesGenomic nucleic acidsAdaptor protein MyD88Endocytic pathwayLigand recognitionCpG contentViral infectionTLR adaptor protein MyD88Innate immune responseToll-like receptor 7Molecular signaturesPlasmacytoid dendritic cellsInnate immune cellsProduction of cytokinesGenomeProtein MyD88Types of pathogensNucleic acidsVivo infection