2019
Application of a Modified Smart-seq2 Sample Preparation Protocol for Rare Cell Full-length Single-cell mRNA Sequencing to Mouse Oocytes.
Treger RS, Pope SD, Xing X, Iwasaki A. Application of a Modified Smart-seq2 Sample Preparation Protocol for Rare Cell Full-length Single-cell mRNA Sequencing to Mouse Oocytes. Bio-protocol 2019, 9: e3345. PMID: 33654848, PMCID: PMC7854224, DOI: 10.21769/bioprotoc.3345.Peer-Reviewed Original ResearchEndogenous retrovirusesFull-length cDNA libraryRNA sequencing librariesSmart-seq2 protocolSingle-cell mRNALibrary preparation methodsMurine genomeCDNA librarySequencing librariesExpression analysisERV expressionPrecise regulationGerm cellsIsolate populationMouse oocytesSubsequent sequencingOocytesMature oocytesSample preparation protocolFamily membersRetroelementsGenomeEmbryogenesisLibraryPreparation protocol
2014
A Promiscuous Lipid-Binding Protein Diversifies the Subcellular Sites of Toll-like Receptor Signal Transduction
Bonham KS, Orzalli MH, Hayashi K, Wolf AI, Glanemann C, Weninger W, Iwasaki A, Knipe DM, Kagan JC. A Promiscuous Lipid-Binding Protein Diversifies the Subcellular Sites of Toll-like Receptor Signal Transduction. Cell 2014, 156: 705-716. PMID: 24529375, PMCID: PMC3951743, DOI: 10.1016/j.cell.2014.01.019.Peer-Reviewed Original ResearchConceptsToll-like receptorsToll-like receptor signal transductionSignal transductionDifferent organellesProinflammatory cytokine expressionSubcellular sitesInnate immune signal transductionInnate immune systemPhosphoinositide-binding domainsImmune signal transductionLipid binding proteinMultiple subcellular locationsReceptor signal transductionCytokine expressionLipid targetsImmune systemInnate immunityHost defenseProtein complexesSubcellular locationPlasma membraneAdaptor TIRAPTIRAPNatural activatorFamily members