2019
The Impact of Single and Multiple Sperm Abnormalities and Low-Level Leukocytospermia on Sperm DNA
Alshahrani S, Ahmad G, Khan H, Ayaz A, Ali A. The Impact of Single and Multiple Sperm Abnormalities and Low-Level Leukocytospermia on Sperm DNA. Journal Of Men's Health 2019, 15: e51-e58. DOI: 10.22374/jomh.v15i2.144.Peer-Reviewed Original ResearchStandard sperm parametersSperm parametersDNA damageAnalysis of semen characteristicsCohort of infertile menSperm DNA testingSperm DNA damageDNA fragmentation analysisLevel of leukocytospermiaNick end labelingSperm motilityInfertile menSemen characteristicsSpermSperm abnormalitiesSperm DNASemen abnormalitiesPercentage of DNA damageRetrospective analysisFragment analysisDNAAbnormal parametersART treatmentEnd labelingDNA testing
2018
Proteomic analysis of sperm proteins in infertile men with high levels of reactive oxygen species
Ayaz A, Agarwal A, Sharma R, Kothandaraman N, Cakar Z, Sikka S. Proteomic analysis of sperm proteins in infertile men with high levels of reactive oxygen species. Andrologia 2018, 50: e13015. PMID: 29656391, DOI: 10.1111/and.13015.Peer-Reviewed Original ResearchMeSH KeywordsAdultAmyloid beta-Protein PrecursorAntigens, NeoplasmF-Box ProteinsHSP70 Heat-Shock ProteinsHumansInfertility, MaleMaleMAP Kinase Kinase Kinase 3Nerve Tissue ProteinsNitric Oxide Synthase Type IIPeptidyl-Dipeptidase AProteomicsReactive Oxygen SpeciesRibosomal ProteinsSmall Ubiquitin-Related Modifier ProteinsSpermatozoaTestisUbiquitinsConceptsReactive oxygen speciesSperm proteinsMale infertilityAngiotensin-converting enzymePost-acrosomal regionPoor sperm qualityLevels of reactive oxygen speciesSperm qualityTesticular environmentInfertile menHeat shock proteinsProteomic analysisHSPA2SpermatozoaExpression profilesOxidative stressRPS27aProteinOxygen speciesMAP3K3Western blottingNeck areaRisk factorsUBL4ASperm
2016
Post-Translational Modifications in sperm Proteome: The Chemistry of Proteome diversifications in the Pathophysiology of male factor infertility
Samanta L, Swain N, Ayaz A, Venugopal V, Agarwal A. Post-Translational Modifications in sperm Proteome: The Chemistry of Proteome diversifications in the Pathophysiology of male factor infertility. Biochimica Et Biophysica Acta 2016, 1860: 1450-1465. PMID: 27062907, DOI: 10.1016/j.bbagen.2016.04.001.Peer-Reviewed Original ResearchConceptsPost-translational modificationsSperm proteinsSperm functionOccurrence of post-translational modificationsExpressed protein complementHuman sperm proteinsProtein synthesis machineryAnalysis of post-translational modificationsComplex interaction networksProteome diversificationSperm proteomeFemale reproductive tractHuman spermatozoaProtein complementStructure-function relationshipsSynthesis machineryInteraction networkProtein stabilityMale infertilitySpermMale factor infertilitySpermatozoaProtein profilesDifferential occurrenceFertility potential
2014
Infertile men older than 40 years are at higher risk of sperm DNA damage
Alshahrani S, Agarwal A, Assidi M, Abuzenadah A, Durairajanayagam D, Ayaz A, Sharma R, Sabanegh E. Infertile men older than 40 years are at higher risk of sperm DNA damage. Reproductive Biology And Endocrinology 2014, 12: 103. PMID: 25410314, PMCID: PMC4258051, DOI: 10.1186/1477-7827-12-103.Peer-Reviewed Original ResearchConceptsSperm DNA damageRisk of sperm DNA damageInfertile menReactive oxygen speciesDNA damagePercentage of sperm DNA fragmentationLevels of sperm DNA damageSemen analysisSeminal reactive oxygen speciesPaternal ageSperm DNA fragmentationAge groupsAssociated with higher riskPaternal age >DNA fragmentationSperm parametersAdvanced paternal ageSemen parametersSemen qualityEffect of paternal ageSpermRetrospective studyGroup BMethodsA totalGroup A