2019
The Impact of Single and Multiple Sperm Abnormalities and Low-Level Leukocytospermia on Sperm DNA
Alshahrani S, Ahmad G, Khan H, Ayaz A, Ali A. The Impact of Single and Multiple Sperm Abnormalities and Low-Level Leukocytospermia on Sperm DNA. Journal Of Men's Health 2019, 15: e51-e58. DOI: 10.22374/jomh.v15i2.144.Peer-Reviewed Original ResearchStandard sperm parametersSperm parametersDNA damageAnalysis of semen characteristicsCohort of infertile menSperm DNA testingSperm DNA damageDNA fragmentation analysisLevel of leukocytospermiaNick end labelingSperm motilityInfertile menSemen characteristicsSpermSperm abnormalitiesSperm DNASemen abnormalitiesPercentage of DNA damageRetrospective analysisFragment analysisDNAAbnormal parametersART treatmentEnd labelingDNA testing
2018
Proteomic analysis of sperm proteins in infertile men with high levels of reactive oxygen species
Ayaz A, Agarwal A, Sharma R, Kothandaraman N, Cakar Z, Sikka S. Proteomic analysis of sperm proteins in infertile men with high levels of reactive oxygen species. Andrologia 2018, 50: e13015. PMID: 29656391, DOI: 10.1111/and.13015.Peer-Reviewed Original ResearchMeSH KeywordsAdultAmyloid beta-Protein PrecursorAntigens, NeoplasmF-Box ProteinsHSP70 Heat-Shock ProteinsHumansInfertility, MaleMaleMAP Kinase Kinase Kinase 3Nerve Tissue ProteinsNitric Oxide Synthase Type IIPeptidyl-Dipeptidase AProteomicsReactive Oxygen SpeciesRibosomal ProteinsSmall Ubiquitin-Related Modifier ProteinsSpermatozoaTestisUbiquitinsConceptsReactive oxygen speciesSperm proteinsMale infertilityAngiotensin-converting enzymePost-acrosomal regionPoor sperm qualityLevels of reactive oxygen speciesSperm qualityTesticular environmentInfertile menHeat shock proteinsProteomic analysisHSPA2SpermatozoaExpression profilesOxidative stressRPS27aProteinOxygen speciesMAP3K3Western blottingNeck areaRisk factorsUBL4ASperm
2015
Proteomic analysis of seminal plasma of infertile men with differing levels of reactive oxygen species reveals defective protein turnover
Agarwal A, Ayaz A, Sharma R, Samanta L, Assidi M, Gupta S, Sabanegh E. Proteomic analysis of seminal plasma of infertile men with differing levels of reactive oxygen species reveals defective protein turnover. Fertility And Sterility 2015, 104: e144. DOI: 10.1016/j.fertnstert.2015.07.445.Peer-Reviewed Original ResearchComparative proteomic pathway analysis of different phenotypes of ejaculated spermatozoa in fertile and infertile men
Agarwal A, Cui Z, Sharma R, Samanta L, Gupta S, Ayaz A, Sabanegh E. Comparative proteomic pathway analysis of different phenotypes of ejaculated spermatozoa in fertile and infertile men. Fertility And Sterility 2015, 104: e297-e298. DOI: 10.1016/j.fertnstert.2015.07.931.Peer-Reviewed Original ResearchComparative proteomic network signatures in seminal plasma of infertile men as a function of reactive oxygen species
Agarwal A, Ayaz A, Samanta L, Sharma R, Assidi M, Abuzenadah A, Sabanegh E. Comparative proteomic network signatures in seminal plasma of infertile men as a function of reactive oxygen species. Clinical Proteomics 2015, 12: 23. PMID: 26321892, PMCID: PMC4552280, DOI: 10.1186/s12014-015-9094-5.Peer-Reviewed Original ResearchMembrane metallo-endopeptidaseInfertile menSeminal plasmaSperm functionFertile menLTQ-Orbitrap Elite hybrid mass spectrometer systemProtein foldingNatural reservoir of antioxidantsMale infertilityROS levelsComparison to fertile controlsSeminal plasma of infertile menPost-translational modification of proteinsHigh ROS groupImpairment of sperm functionPathology of male infertilitySeminal plasma proteinsDiagnosis of male infertilityFunction of reactive oxygen speciesMotility of spermPost-translational modificationsFertile groupModification of proteinsInfertile patient groupsOxygen speciesSpermatozoa protein alterations in infertile men with bilateral varicocele
Agarwal A, Sharma R, Durairajanayagam D, Cui Z, Ayaz A, Gupta S, Willard B, Gopalan B, Sabanegh E. Spermatozoa protein alterations in infertile men with bilateral varicocele. Asian Journal Of Andrology 2015, 18: 43-53. PMID: 25999357, PMCID: PMC4736356, DOI: 10.4103/1008-682x.153848.Peer-Reviewed Original ResearchConceptsInfertile menBilateral varicoceleFertile menAssociated with metabolic processesSperm-zona bindingVaricocele casesFunctional proteomic profilingVaricocele-associated male infertilityImmune system processPresence of DEPSperm-zonaSperm functionFertile control groupCells of patientsFertile donorsEnzyme regulationIdentified proteinsOxidoreductase activityMale infertilitySpermatozoa cellsProteomic analysisWestern blot analysisStress responseMetabolic processesProteomic profilingDifferential Proteomic Profiling of Spermatozoal Proteins of Infertile Men With Unilateral or Bilateral Varicocele
Agarwal A, Sharma R, Durairajanayagam D, Cui Z, Ayaz A, Gupta S, Willard B, Gopalan B, Sabanegh E. Differential Proteomic Profiling of Spermatozoal Proteins of Infertile Men With Unilateral or Bilateral Varicocele. Urology 2015, 85: 580-588. PMID: 25733269, DOI: 10.1016/j.urology.2014.11.030.Peer-Reviewed Original ResearchConceptsSperm protein profilePost-translational modificationsInfertile menRoutine sperm parametersProtein profilesSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisFunctional proteomic profilingGel analysis softwareVaricocele-associated male infertilitySperm deoxyribonucleic acid fragmentationComparing spectral countsVaricocele samplesDeoxyribonucleic acid fragmentationProtein foldingSperm parametersDifferential proteomic profilingProtein ubiquitinationCellular functionsSignal transductionGel electrophoresisExpressed proteinsMale infertilityReactive oxygen speciesMajor protein alterations in spermatozoa from infertile men with unilateral varicocele
Agarwal A, Sharma R, Durairajanayagam D, Ayaz A, Cui Z, Willard B, Gopalan B, Sabanegh E. Major protein alterations in spermatozoa from infertile men with unilateral varicocele. Reproductive Biology And Endocrinology 2015, 13: 8. PMID: 25890347, PMCID: PMC4383193, DOI: 10.1186/s12958-015-0007-2.Peer-Reviewed Original ResearchConceptsInfertile menVaricocele groupLTQ-Orbitrap Elite hybrid mass spectrometer systemAcquisition of sperm motilityPost-translational modification proteinsFertile groupCause of male factor infertilitySmall molecule biochemistryAcrosome reactionSperm maturationUnilateral varicoceleFunctional annotationModification proteinsReactive oxygen species levelsSperm motilityResultsSperm concentrationBioinformatics toolsSpecies levelFertile menSignal transductionProteomic changesFunctional pathwaysBioinformatics analysisAnalyzed proteinsGene expressionImpact of precise modulation of reactive oxygen species levels on spermatozoa proteins in infertile men
Ayaz A, Agarwal A, Sharma R, Arafa M, Elbardisi H, Cui Z. Impact of precise modulation of reactive oxygen species levels on spermatozoa proteins in infertile men. Clinical Proteomics 2015, 12: 4. PMID: 25972767, PMCID: PMC4429661, DOI: 10.1186/1559-0275-12-4.Peer-Reviewed Original ResearchReactive oxygen species levelsInfertile menReactive oxygen speciesLevels of reactive oxygen speciesMale infertilityROS groupHigher ROS levelsHigher reactive oxygen speciesFertile menExpression of sperm proteinsHigh ROS groupPathology of male infertilityROS levelsGlobal proteomic analysisSperm proteinsSpermatozoa proteinsReactive oxygen species groupIn-gel digestionBioinformatics toolsSpecies levelCellular processesExpressed proteinsReproductive functionProteomic analysisIn-gel
2014
Infertile men older than 40 years are at higher risk of sperm DNA damage
Alshahrani S, Agarwal A, Assidi M, Abuzenadah A, Durairajanayagam D, Ayaz A, Sharma R, Sabanegh E. Infertile men older than 40 years are at higher risk of sperm DNA damage. Reproductive Biology And Endocrinology 2014, 12: 103. PMID: 25410314, PMCID: PMC4258051, DOI: 10.1186/1477-7827-12-103.Peer-Reviewed Original ResearchConceptsSperm DNA damageRisk of sperm DNA damageInfertile menReactive oxygen speciesDNA damagePercentage of sperm DNA fragmentationLevels of sperm DNA damageSemen analysisSeminal reactive oxygen speciesPaternal ageSperm DNA fragmentationAge groupsAssociated with higher riskPaternal age >DNA fragmentationSperm parametersAdvanced paternal ageSemen parametersSemen qualityEffect of paternal ageSpermRetrospective studyGroup BMethodsA totalGroup AEffect of advancing paternal age on semen parameters and seminal oxidative stress markers in infertile men
Alshahrani S, Agarwal A, Assidi M, Abuzenadah A, Durairajanayagam D, Ayaz A, Sharma R, Sabanegh E. Effect of advancing paternal age on semen parameters and seminal oxidative stress markers in infertile men. BMC Genomics 2014, 15: p42. PMCID: PMC4075617, DOI: 10.1186/1471-2164-15-s2-p42.Peer-Reviewed Original ResearchImpact of World Health Organization (WHO) new standards on the referral pattern of infertile men for assisted reproduction
Alshahrani S, Agarwal A, Assidi M, Abuzenadah A, Durairajanayagam D, Ayaz A, Sharma R. Impact of World Health Organization (WHO) new standards on the referral pattern of infertile men for assisted reproduction. BMC Genomics 2014, 15: p31. PMCID: PMC4075603, DOI: 10.1186/1471-2164-15-s2-p31.Peer-Reviewed Original Research