2013
Insecticide Resistance Allele Frequencies in Anopheles gambiae before and after Anti-Vector Interventions in Continental Equatorial Guinea
Reddy MR, Godoy A, Dion K, Matias A, Callender K, Kiszewski AE, Kleinschmidt I, Ridl FC, Powell JR, Caccone A, Slotman MA. Insecticide Resistance Allele Frequencies in Anopheles gambiae before and after Anti-Vector Interventions in Continental Equatorial Guinea. American Journal Of Tropical Medicine And Hygiene 2013, 88: 897-907. PMID: 23438768, PMCID: PMC3752755, DOI: 10.4269/ajtmh.12-0467.Peer-Reviewed Original Research
2012
The Effective Population Size of Malaria Mosquitoes: Large Impact of Vector Control
Athrey G, Hodges T, Reddy M, Overgaard H, Matias A, Ridl F, Kleinschmidt I, Caccone A, Slotman M. The Effective Population Size of Malaria Mosquitoes: Large Impact of Vector Control. PLOS Genetics 2012, 8: e1003097. PMID: 23271973, PMCID: PMC3521722, DOI: 10.1371/journal.pgen.1003097.Peer-Reviewed Original ResearchGenetic isolation within the malaria mosquito Anopheles melas
Deitz K, Athrey G, Reddy M, Overgaard H, Matias A, Jawara M, della Torre A, Petrarca V, Pinto J, Kiszewski A, Kengne P, Costantini C, Caccone A, Slotman M. Genetic isolation within the malaria mosquito Anopheles melas. Molecular Ecology 2012, 21: 4498-4513. PMID: 22882458, PMCID: PMC4067978, DOI: 10.1111/j.1365-294x.2012.05724.x.Peer-Reviewed Original ResearchConceptsMicrosatellite dataIsland populationsGambiae complexMaximum likelihood phylogenetic analysisBp of mtDNALevel of divergenceLast glaciation periodA. gambiae complexMalaria vectorsSalt marsh grassAnopheles gambiae complexAnopheles melasMajor malaria vectorApproximate Bayesian analysisMtDNA divergenceGenetic differentiationGenetic divergenceGenetic isolationMainland populationsPhylogenetic analysisPopulation structureMicrosatellite markersLarval habitatsA. gambiaeGlaciation periodLimited Usefulness of Microsatellite Markers from the Malaria Vector Anophelesgambiae When Applied to the Closely Related Species Anopheles melas
Deitz K, Reddy V, Reddy M, Satyanarayanah N, Lindsey M, Overgaard H, Jawara M, Caccone A, Slotman M. Limited Usefulness of Microsatellite Markers from the Malaria Vector Anophelesgambiae When Applied to the Closely Related Species Anopheles melas. Journal Of Heredity 2012, 103: 585-593. PMID: 22593601, DOI: 10.1093/jhered/ess025.Peer-Reviewed Original Research
2011
Outdoor host seeking behaviour of Anopheles gambiae mosquitoes following initiation of malaria vector control on Bioko Island, Equatorial Guinea
Reddy M, Overgaard H, Abaga S, Reddy V, Caccone A, Kiszewski A, Slotman M. Outdoor host seeking behaviour of Anopheles gambiae mosquitoes following initiation of malaria vector control on Bioko Island, Equatorial Guinea. Malaria Journal 2011, 10: 184. PMID: 21736750, PMCID: PMC3146901, DOI: 10.1186/1475-2875-10-184.Peer-Reviewed Original ResearchEstimation of the Human Blood Index in Malaria Mosquito Vectors in Equatorial Guinea after Indoor Antivector Interventions
Pappa V, Reddy M, Overgaard H, Abaga S, Caccone A. Estimation of the Human Blood Index in Malaria Mosquito Vectors in Equatorial Guinea after Indoor Antivector Interventions. American Journal Of Tropical Medicine And Hygiene 2011, 84: 298-301. PMID: 21292902, PMCID: PMC3029185, DOI: 10.4269/ajtmh.2011.10-0463.Peer-Reviewed Original Research
2010
Anopheles Immune Genes and Amino Acid Sites Evolving Under the Effect of Positive Selection
Parmakelis A, Moustaka M, Poulakakis N, Louis C, Slotman MA, Marshall JC, Awono-Ambene PH, Antonio-Nkondjio C, Simard F, Caccone A, Powell JR. Anopheles Immune Genes and Amino Acid Sites Evolving Under the Effect of Positive Selection. PLOS ONE 2010, 5: e8885. PMID: 20126662, PMCID: PMC2811201, DOI: 10.1371/journal.pone.0008885.Peer-Reviewed Original ResearchConceptsPositive selectionImmune genesGambiae complexDN/dS ratiosLineage-specific evolutionPopulation genetics frameworkAmino acid sitesAnopheles gambiae complexSpecific amino acidsInnate immunity genesAncestral polymorphismComparative phylogeneticsGene diversityEvolutionary historyNatural populationsGenetic diversityGenetic frameworkNatural selectionImmunity genesVector biologyDS ratiosGenesAmino acidsDiversityGenetic knowledge
2008
High Levels of Hybridization between Molecular Forms of Anopheles gambiae from Guinea Bissau
Oliveira E, Salgueiro P, Palsson K, Vicente J, Arez A, Jaenson T, Caccone A, Pinto J. High Levels of Hybridization between Molecular Forms of Anopheles gambiae from Guinea Bissau. Journal Of Medical Entomology 2008, 45: 1057-1063. DOI: 10.1093/jmedent/45.6.1057.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnophelesGuinea-BissauHybridization, GeneticMicrosatellite RepeatsPolymorphism, GeneticConceptsGenetic differentiationIncipient speciationSignificant genetic differentiationSpecies distribution rangeLow recombination regionsDegree of isolationMalaria vector AnophelesSamples of AnChromosome 3Distribution rangeLocus mapsAnopheles gambiaeHigh differentiationS formChromosome XGiles sensu strictoForm compositionSensu strictoVector AnophelesGambiaeMolecular formsS hybridsMicrosatellitesDifferentiationRecombination regionHigh levels of hybridization between molecular forms of Anopheles gambiae from Guinea Bissau.
Oliveira E, Salgueiro P, Palsson K, Vicente J, Arez A, Jaenson T, Caccone A, Pinto J. High levels of hybridization between molecular forms of Anopheles gambiae from Guinea Bissau. Journal Of Medical Entomology 2008, 45: 1057-63. PMID: 19058629, DOI: 10.1603/0022-2585(2008)45[1057:hlohbm]2.0.co;2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnophelesGuinea-BissauHybridization, GeneticMicrosatellite RepeatsPolymorphism, GeneticConceptsGenetic differentiationIncipient speciationSignificant genetic differentiationSpecies distribution rangeLow recombination regionsDegree of isolationMalaria vector AnophelesSamples of AnChromosome 3Distribution rangeLocus mapsAnopheles gambiaeHigh differentiationS formChromosome XGiles sensu strictoForm compositionSensu strictoVector AnophelesGambiaeMolecular formsS hybridsMicrosatellitesDifferentiationRecombination regionDistribution of knock-down resistance mutations in Anopheles gambiae molecular forms in west and west-central Africa
Santolamazza F, Calzetta M, Etang J, Barrese E, Dia I, Caccone A, Donnelly M, Petrarca V, Simard F, Pinto J, della Torre A. Distribution of knock-down resistance mutations in Anopheles gambiae molecular forms in west and west-central Africa. Malaria Journal 2008, 7: 74. PMID: 18445265, PMCID: PMC2405802, DOI: 10.1186/1475-2875-7-74.Peer-Reviewed Original ResearchConceptsL1014F alleleVoltage-gated sodium channel geneM-form populationsSodium channel geneResistance allele frequencyAnopheles gambiae sensu strictoPhenotypic effectsGambiae sensu strictoSelection pressureGambiae ML1014F mutationMolecular formsPosition 1014Channel genesL1014S mutationS formLigation assayL1014S alleleSensu strictoKdr genotypesVector speciesKdr alleleAllele-specific PCRPoint mutationsFormer mutationThe molecular evolution of four anti-malarial immune genes in the Anopheles gambiae species complex
Parmakelis A, Slotman MA, Marshall JC, Awono-Ambene PH, Antonio-Nkondjio C, Simard F, Caccone A, Powell JR. The molecular evolution of four anti-malarial immune genes in the Anopheles gambiae species complex. BMC Ecology And Evolution 2008, 8: 79. PMID: 18325105, PMCID: PMC2288592, DOI: 10.1186/1471-2148-8-79.Peer-Reviewed Original ResearchConceptsInsect innate immune systemAnopheles gambiae speciesLevel of polymorphismAnopheles gambiae complexSpecific adaptive responsesAncestral polymorphismPhylogenetic frameworkMolecular evolutionSuch genesGambiae speciesPositive selectionImmunity genesSelection pressureCandidate genesImmune genesMosquito's abilityGambiae complexStudied genesGenesInnate immune systemAdaptive responseMalaria parasitesPlasmodium parasitesEase of manipulationSpeciesHistorical analysis of a near disaster: Anopheles gambiae in Brazil.
Parmakelis A, Russello MA, Caccone A, Marcondes CB, Costa J, Forattini OP, Sallum MA, Wilkerson RC, Powell JR. Historical analysis of a near disaster: Anopheles gambiae in Brazil. American Journal Of Tropical Medicine And Hygiene 2008, 78: 176-8. PMID: 18187802, DOI: 10.4269/ajtmh.2008.78.176.Peer-Reviewed Original ResearchConceptsHuman-mediated range expansionIntrinsic historical interestAnopheles gambiaeDisease vectorsHistorical DNA analysisHuman-mediated dispersalInsect disease vectorsMuseum collectionsHistorical analysisNear disasterHistorical interestAnopheles gambiae complexHistorical informationTime of invasionIntercontinental introductionSpecies complexSpecies identityRange expansionMuseum specimensGambiae complexGambiaeSpeciesAnopheles arabiensisIdentityDNA analysis
2007
Multiple Origins of Knockdown Resistance Mutations in the Afrotropical Mosquito Vector Anopheles gambiae
Pinto J, Lynd A, Vicente J, Santolamazza F, Randle N, Gentile G, Moreno M, Simard F, Charlwood J, do Rosário V, Caccone A, della Torre A, Donnelly M. Multiple Origins of Knockdown Resistance Mutations in the Afrotropical Mosquito Vector Anopheles gambiae. PLOS ONE 2007, 2: e1243. PMID: 18043750, PMCID: PMC2080755, DOI: 10.1371/journal.pone.0001243.Peer-Reviewed Original ResearchConceptsResistance allelesMutation eventsMosquito vector Anopheles gambiaeVoltage-gated sodium channel geneNatural insect populationsEvolution of resistanceInsecticide resistance mutationsIndependent mutation eventsGambiae S formSodium channel geneMalaria vector AnophelesKnockdown resistance alleleKnockdown resistance (kdr) mutationsGene flowMolecular evolutionNeutrality testsSelective sweepsMalaria vector control programmesAdaptive traitsMultiple mutation eventsInsect populationsGenomic regionsHaplotype compositionKdr locusLocal selectionGenetic population structure of Anopheles gambiae in Equatorial Guinea
Moreno M, Salgueiro P, Vicente J, Cano J, Berzosa P, de Lucio A, Simard F, Caccone A, Do Rosario V, Pinto J, Benito A. Genetic population structure of Anopheles gambiae in Equatorial Guinea. Malaria Journal 2007, 6: 137. PMID: 17937805, PMCID: PMC2100067, DOI: 10.1186/1475-2875-6-137.Peer-Reviewed Original ResearchConceptsGenetic differentiationMicrosatellite lociPopulation structureMainland samplesGenetic diversity parametersGenetic population structureGambiae s.A. gambiae s.Incipient speciationPopulation differentiationGene flowGenetic structureGenetic isolationS formMicrosatellite dataGenomic islandsContinental Equatorial GuineaDiversity parametersMosquito vector populationsChromosome 3Anopheles gambiaeIsland samplesAnopheles gambiae s.Shallow differentiationIsland of BiokoDevelopment of a molecular assay to detect predation on Anopheles gambiae complex larval stages.
Schielke E, Costantini C, Carchini G, Sagnon N, Powell J, Caccone A. Development of a molecular assay to detect predation on Anopheles gambiae complex larval stages. American Journal Of Tropical Medicine And Hygiene 2007, 77: 464-6. PMID: 17827361, DOI: 10.4269/ajtmh.2007.77.464.Peer-Reviewed Original ResearchConceptsIdentification of speciesInvertebrate predatorsRestriction enzyme analysisMosquito DNAFamily LestidaeLarval stagesMolecular assaysAnopheles gambiae sensu lato mosquitoesDNA extractsEnzyme analysisPredationGambiae s.DNAPolymerase chain reaction assaysAssaysChain reaction assaysMolecular formsNotonectidaeLibellulidaePredatorsLestidaeSpeciesReaction assaysAnophelesAnPatterns of Selection in Anti-Malarial Immune Genes in Malaria Vectors: Evidence for Adaptive Evolution in LRIM1 in Anopheles arabiensis
Slotman MA, Parmakelis A, Marshall JC, Awono-Ambene PH, Antonio-Nkondjo C, Simard F, Caccone A, Powell JR. Patterns of Selection in Anti-Malarial Immune Genes in Malaria Vectors: Evidence for Adaptive Evolution in LRIM1 in Anopheles arabiensis. PLOS ONE 2007, 2: e793. PMID: 17726523, PMCID: PMC1945087, DOI: 10.1371/journal.pone.0000793.Peer-Reviewed Original ResearchConceptsAdaptive evolutionPrimary malaria vectorMalaria vectorsPatterns of selectionNon-vector speciesAnopheles gambiae complexMcDonald-KreitmanReplacement substitutionsPositive selectionGenetic variationMalaria vector AnLRIM1Immune genesSelection showGambiae complexGenesAdjacent codonsProtein differentiationLevel of resistanceAdaptive responsePlasmodium speciesCEC1Vector AnMaximum likelihood testSpecies
2006
Genetic differentiation between the BAMAKO and SAVANNA chromosomal forms of Anopheles gambiae as indicated by amplified fragment length polymorphism analysis.
SLOTMAN M, MENDEZ M, Torre A, Dolo G, TOURÉ Y, Caccone A. Genetic differentiation between the BAMAKO and SAVANNA chromosomal forms of Anopheles gambiae as indicated by amplified fragment length polymorphism analysis. American Journal Of Tropical Medicine And Hygiene 2006, 74: 641-8. PMID: 16606999, DOI: 10.4269/ajtmh.2006.74.641.Peer-Reviewed Original ResearchConceptsChromosomal formsAnopheles gambiaeSavanna chromosomal formsRibosomal DNA locusFragment length polymorphism (AFLP) primer combinationsPolymorphism primer combinationsGenetic differentiationGene flowDNA lociAFLP analysisFragment length polymorphism analysisPrimer combinationsMolecular markersDiagnostic differencesRecent originLength polymorphism analysisSavannaGambiaePolymorphism analysisMain vectorSympatryGenomeLociDifferentiationMoptiCo‐occurrence of East and West African kdr mutations suggests high levels of resistance to pyrethroid insecticides in Anopheles gambiae from Libreville, Gabon
Pinto J, Lynd A, Elissa N, Donnelly M, Costa C, Gentile G, Caccone A, DO Rosário V. Co‐occurrence of East and West African kdr mutations suggests high levels of resistance to pyrethroid insecticides in Anopheles gambiae from Libreville, Gabon. Medical And Veterinary Entomology 2006, 20: 27-32. PMID: 16608487, DOI: 10.1111/j.1365-2915.2006.00611.x.Peer-Reviewed Original Research
2005
Short report: Phylogenetic relationships of the anthropophilic Plasmodium falciparum malaria vectors in Africa.
MARSHALL J, POWELL J, Caccone A. Short report: Phylogenetic relationships of the anthropophilic Plasmodium falciparum malaria vectors in Africa. American Journal Of Tropical Medicine And Hygiene 2005, 73: 749-52. PMID: 16222020, DOI: 10.4269/ajtmh.2005.73.749.Peer-Reviewed Original ResearchConceptsNumber of speciesMalaria vectorsAfrican mosquito vectorsMosquito vectorsNuclear genesParaphyletic relationshipSubgenus CelliaPhylogenetic relationshipsPhylogenetic analysisAdditional speciesStatistical supportSpeciesPlasmodium falciparumP. falciparumCausative agentCelliaFalciparumGenesHistorical associationVectorBayesian methods
2004
Variation in an intron sequence of the voltage‐gated sodium channel gene correlates with genetic differentiation between Anopheles gambiae s.s. molecular forms
Gentile G, Santolamazza F, Fanello C, Petrarca V, Caccone A, Della Torre A. Variation in an intron sequence of the voltage‐gated sodium channel gene correlates with genetic differentiation between Anopheles gambiae s.s. molecular forms. Insect Molecular Biology 2004, 13: 371-377. PMID: 15271209, DOI: 10.1111/j.0962-1075.2004.00494.x.Peer-Reviewed Original ResearchConceptsVoltage-gated sodium channel geneSodium channel geneGenetic differentiationGenetic variationChannel genesPossible selective sweepMajor haplotypesSingle mutational stepAnopheles gambiae MRDNA sitesGene flowSelective sweepsMutational stepsTaxonomic statusIntron sequencesGambiae MMolecular formsIntron ISingle localityIntronic regionsAnopheles gambiae s.Additional haplotypesGambiae s.HaplotypesGenes