2020
Improved reference genome of the arboviral vector Aedes albopictus
Palatini U, Masri RA, Cosme LV, Koren S, Thibaud-Nissen F, Biedler JK, Krsticevic F, Johnston JS, Halbach R, Crawford JE, Antoshechkin I, Failloux AB, Pischedda E, Marconcini M, Ghurye J, Rhie A, Sharma A, Karagodin DA, Jenrette J, Gamez S, Miesen P, Masterson P, Caccone A, Sharakhova MV, Tu Z, Papathanos PA, Van Rij RP, Akbari OS, Powell J, Phillippy AM, Bonizzoni M. Improved reference genome of the arboviral vector Aedes albopictus. Genome Biology 2020, 21: 215. PMID: 32847630, PMCID: PMC7448346, DOI: 10.1186/s13059-020-02141-w.Peer-Reviewed Original ResearchConceptsArboviral vector Aedes albopictusSex-determining locusFirst physical mapAsian tiger mosquito Aedes albopictusTiger mosquito Aedes albopictusMosquito Aedes albopictusAedes albopictusPiRNA clustersDiverse AeGenome assemblyInnovative control measuresReference genomeAccurate DNA sequencesNovel microRNAsImmunity genesPhysical mapDNA sequencesGenetic manipulationViral insertionVector Aedes albopictusExpression dataGenomeAlbopictus populationsSequencing methodsAdaptation potentialPhylogeography and population structure of the tsetse fly Glossina pallidipes in Kenya and the Serengeti ecosystem
Bateta R, Saarman NP, Okeyo WA, Dion K, Johnson T, Mireji PO, Okoth S, Malele I, Murilla G, Aksoy S, Caccone A. Phylogeography and population structure of the tsetse fly Glossina pallidipes in Kenya and the Serengeti ecosystem. PLOS Neglected Tropical Diseases 2020, 14: e0007855. PMID: 32092056, PMCID: PMC7058365, DOI: 10.1371/journal.pntd.0007855.Peer-Reviewed Original Research
2019
Complex interplay of evolutionary forces shaping population genomic structure of invasive Aedes albopictus in southern Europe
Pichler V, Kotsakiozi P, Caputo B, Serini P, Caccone A, della Torre A. Complex interplay of evolutionary forces shaping population genomic structure of invasive Aedes albopictus in southern Europe. PLOS Neglected Tropical Diseases 2019, 13: e0007554. PMID: 31437154, PMCID: PMC6705758, DOI: 10.1371/journal.pntd.0007554.Peer-Reviewed Original ResearchConceptsDouble digest Restriction-site Associated DNAHistorical contextMultiple independent invasionsSouth East AsiaRestriction-site Associated DNAPopulation genomic structureInvasive Aedes albopictusInvasion historyWorldwide expansionSouthern EuropeFirst countryHistoryAnimal invasionsItalyEuropeAlbaniaSuccessful historyRecent invasionMigration patternsHuman arbovirusesComplex interplayMultiple geographic locationsIndependent invasionsColonistsInvasive rangeSpatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda
Schneider DI, Saarman N, Onyango MG, Hyseni C, Opiro R, Echodu R, O’Neill M, Bloch D, Vigneron A, Johnson TJ, Dion K, Weiss BL, Opiyo E, Caccone A, Aksoy S. Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda. PLOS Neglected Tropical Diseases 2019, 13: e0007340. PMID: 31369548, PMCID: PMC6692048, DOI: 10.1371/journal.pntd.0007340.Peer-Reviewed Original Research
2018
Genetic Differentiation of Glossina pallidipes Tsetse Flies in Southern Kenya.
Okeyo WA, Saarman NP, Bateta R, Dion K, Mengual M, Mireji PO, Ouma C, Okoth S, Murilla G, Aksoy S, Caccone A. Genetic Differentiation of Glossina pallidipes Tsetse Flies in Southern Kenya. American Journal Of Tropical Medicine And Hygiene 2018, 99: 945-953. PMID: 30105964, PMCID: PMC6159567, DOI: 10.4269/ajtmh.18-0154.Peer-Reviewed Original ResearchConceptsGenetic differentiationPopulation dynamicsDispersal patternsTsetse fliesLow genetic diversityGreater genetic differentiationEvidence of admixtureWestern clusterEastern clusterRecent anthropogenic influencesAnimal African trypanosomiasisGenetic connectivityWestern relativesGenetic diversityMicrosatellite lociPopulation structureGenotypic dataWidespread signalDisease vectorsMajor vectorVector control effortsGreat Rift ValleyDemographic parametersFliesVector control programs
2017
Temporal genetic differentiation in Glossina pallidipes tsetse fly populations in Kenya
Okeyo WA, Saarman NP, Mengual M, Dion K, Bateta R, Mireji PO, Okoth S, Ouma JO, Ouma C, Ochieng J, Murilla G, Aksoy S, Caccone A. Temporal genetic differentiation in Glossina pallidipes tsetse fly populations in Kenya. Parasites & Vectors 2017, 10: 471. PMID: 29017572, PMCID: PMC5635580, DOI: 10.1186/s13071-017-2415-y.Peer-Reviewed Original ResearchConceptsTemporal genetic differentiationGenetic diversityGenetic differentiationGenetic bottleneckGenetic variationNational ParkTemporal genetic variationExtensive control measuresAnimal African trypanosomiasisAllelic richnessPairwise FSTGenetic driftNe estimatesHabitat alterationPopulation geneticsTemporal differentiationMicrosatellite markersRuma National ParkClosest sampling sitesG. pallidipesMajor vectorTsetse fliesVector populationsDifferentiationDiversityGenetic diversity of Glossina fuscipes fuscipes along the shores of Lake Victoria in Tanzania and Kenya: implications for management
Manangwa O, Nkwengulila G, Ouma JO, Mramba F, Malele I, Dion K, Sistrom M, Khan F, Aksoy S, Caccone A. Genetic diversity of Glossina fuscipes fuscipes along the shores of Lake Victoria in Tanzania and Kenya: implications for management. Parasites & Vectors 2017, 10: 268. PMID: 28558831, PMCID: PMC5450392, DOI: 10.1186/s13071-017-2201-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGenetic VariationInsect VectorsKenyaLakesMicrosatellite RepeatsTanzaniaTsetse FliesGenetic diversity and population structure of the tsetse fly Glossina fuscipes fuscipes (Diptera: Glossinidae) in Northern Uganda: Implications for vector control
Opiro R, Saarman NP, Echodu R, Opiyo EA, Dion K, Halyard A, Dunn AW, Aksoy S, Caccone A. Genetic diversity and population structure of the tsetse fly Glossina fuscipes fuscipes (Diptera: Glossinidae) in Northern Uganda: Implications for vector control. PLOS Neglected Tropical Diseases 2017, 11: e0005485. PMID: 28453513, PMCID: PMC5425221, DOI: 10.1371/journal.pntd.0005485.Peer-Reviewed Original ResearchConceptsAnimal African trypanosomiasisGenetic clustersEffective population size estimatesLake KyogaRecent population bottleneckMitochondrial DNA markersDistinct genetic clustersPolymorphic microsatellite lociNile drainagePopulation size estimatesGenetic structurePopulation bottlenecksMain haplogroupsGenetic diversityMicrosatellite lociGenetic assignmentDNA markersPopulation structureMtDNA analysisGenetic admixtureSampling sitesGenetic unitsMost populationsTrypanosoma parasitesHuman African trypanosomiasis
2016
Vectors as Epidemiological Sentinels: Patterns of Within-Tick Borrelia burgdorferi Diversity
Walter KS, Carpi G, Evans BR, Caccone A, Diuk-Wasser MA. Vectors as Epidemiological Sentinels: Patterns of Within-Tick Borrelia burgdorferi Diversity. PLOS Pathogens 2016, 12: e1005759. PMID: 27414806, PMCID: PMC4944968, DOI: 10.1371/journal.ppat.1005759.Peer-Reviewed Original ResearchConceptsPathogen diversityPositive selectionVertebrate hostsHost pathogen diversityLyme disease bacteriaNatural transmission cycleNatural populationsPathogen evolutionGenomic variationHost diversityMajority of ticksHost processesDiverse inoculumUndocumented levelEvolutionary processesTick diversityDisease vectorsMixed strain infectionsImmune complementIndividual ticksDiversityEpidemiological sentinelsTick vectorTransmission cycleHost
2013
Genetically Distinct Glossina fuscipes fuscipes Populations in the Lake Kyoga Region of Uganda and Its Relevance for Human African Trypanosomiasis
Echodu R, Sistrom M, Hyseni C, Enyaru J, Okedi L, Aksoy S, Caccone A. Genetically Distinct Glossina fuscipes fuscipes Populations in the Lake Kyoga Region of Uganda and Its Relevance for Human African Trypanosomiasis. BioMed Research International 2013, 2013: 614721. PMID: 24199195, PMCID: PMC3807537, DOI: 10.1155/2013/614721.Peer-Reviewed Original ResearchConceptsSex-biased dispersalMicrosatellite DNA dataLack of admixtureAgents of humanVicariant barrierGenetic differentiationSouthern populationsDNA dataTrypanosoma bruceiCompetitive exclusionMain vector speciesSole vectorsContact zonePopulation clustersVector speciesEnvironmental conditionsTsetse fliesDistinct groupsAnimal trypanosomiasisHuman African trypanosomiasisGlossinaAfrican trypanosomiasisIntrogressionSympatryDispersalGlossina fuscipes populations provide insights for human African trypanosomiasis transmission in Uganda
Aksoy S, Caccone A, Galvani AP, Okedi LM. Glossina fuscipes populations provide insights for human African trypanosomiasis transmission in Uganda. Trends In Parasitology 2013, 29: 394-406. PMID: 23845311, PMCID: PMC3772539, DOI: 10.1016/j.pt.2013.06.005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiodiversityDNA, MitochondrialFemaleGene FlowGenetics, PopulationHost-Pathogen InteractionsHumansInsect VectorsMaleMicrobiotaMicrosatellite RepeatsPest Control, BiologicalSymbiosisTrypanosoma brucei gambienseTrypanosoma brucei rhodesienseTrypanosomiasis, AfricanTsetse FliesUgandaConceptsOccurrence of polyandryGene flowPopulation geneticsPopulation structureGenetic applicationsHuman African trypanosomiasisMajor vectorMicrobiome compositionTsetse control programsTrypanosomiasis transmissionRecent spreadPotential rolePresent knowledgeGlossinaAfrican trypanosomiasisPolyandryHuman African trypanosomiasis transmissionInfection outcomesGeneticsFuscipesDiversityPopulationInsecticide Resistance Allele Frequencies in Anopheles gambiae before and after Anti-Vector Interventions in Continental Equatorial Guinea
Reddy MR, Godoy A, Dion K, Matias A, Callender K, Kiszewski AE, Kleinschmidt I, Ridl FC, Powell JR, Caccone A, Slotman MA. Insecticide Resistance Allele Frequencies in Anopheles gambiae before and after Anti-Vector Interventions in Continental Equatorial Guinea. American Journal Of Tropical Medicine And Hygiene 2013, 88: 897-907. PMID: 23438768, PMCID: PMC3752755, DOI: 10.4269/ajtmh.12-0467.Peer-Reviewed Original Research
2012
The Effective Population Size of Malaria Mosquitoes: Large Impact of Vector Control
Athrey G, Hodges T, Reddy M, Overgaard H, Matias A, Ridl F, Kleinschmidt I, Caccone A, Slotman M. The Effective Population Size of Malaria Mosquitoes: Large Impact of Vector Control. PLOS Genetics 2012, 8: e1003097. PMID: 23271973, PMCID: PMC3521722, DOI: 10.1371/journal.pgen.1003097.Peer-Reviewed Original Research
2011
Estimation of the Human Blood Index in Malaria Mosquito Vectors in Equatorial Guinea after Indoor Antivector Interventions
Pappa V, Reddy M, Overgaard H, Abaga S, Caccone A. Estimation of the Human Blood Index in Malaria Mosquito Vectors in Equatorial Guinea after Indoor Antivector Interventions. American Journal Of Tropical Medicine And Hygiene 2011, 84: 298-301. PMID: 21292902, PMCID: PMC3029185, DOI: 10.4269/ajtmh.2011.10-0463.Peer-Reviewed Original Research
2008
High Levels of Genetic Differentiation between Ugandan Glossina fuscipes fuscipes Populations Separated by Lake Kyoga
Abila PP, Slotman MA, Parmakelis A, Dion KB, Robinson AS, Muwanika VB, Enyaru JC, Lokedi L, Aksoy S, Caccone A. High Levels of Genetic Differentiation between Ugandan Glossina fuscipes fuscipes Populations Separated by Lake Kyoga. PLOS Neglected Tropical Diseases 2008, 2: e242. PMID: 18509474, PMCID: PMC2386243, DOI: 10.1371/journal.pntd.0000242.Peer-Reviewed Original ResearchDistribution of knock-down resistance mutations in Anopheles gambiae molecular forms in west and west-central Africa
Santolamazza F, Calzetta M, Etang J, Barrese E, Dia I, Caccone A, Donnelly M, Petrarca V, Simard F, Pinto J, della Torre A. Distribution of knock-down resistance mutations in Anopheles gambiae molecular forms in west and west-central Africa. Malaria Journal 2008, 7: 74. PMID: 18445265, PMCID: PMC2405802, DOI: 10.1186/1475-2875-7-74.Peer-Reviewed Original ResearchConceptsL1014F alleleVoltage-gated sodium channel geneM-form populationsSodium channel geneResistance allele frequencyAnopheles gambiae sensu strictoPhenotypic effectsGambiae sensu strictoSelection pressureGambiae ML1014F mutationMolecular formsPosition 1014Channel genesL1014S mutationS formLigation assayL1014S alleleSensu strictoKdr genotypesVector speciesKdr alleleAllele-specific PCRPoint mutationsFormer mutationHistorical analysis of a near disaster: Anopheles gambiae in Brazil.
Parmakelis A, Russello MA, Caccone A, Marcondes CB, Costa J, Forattini OP, Sallum MA, Wilkerson RC, Powell JR. Historical analysis of a near disaster: Anopheles gambiae in Brazil. American Journal Of Tropical Medicine And Hygiene 2008, 78: 176-8. PMID: 18187802, DOI: 10.4269/ajtmh.2008.78.176.Peer-Reviewed Original ResearchConceptsHuman-mediated range expansionIntrinsic historical interestAnopheles gambiaeDisease vectorsHistorical DNA analysisHuman-mediated dispersalInsect disease vectorsMuseum collectionsHistorical analysisNear disasterHistorical interestAnopheles gambiae complexHistorical informationTime of invasionIntercontinental introductionSpecies complexSpecies identityRange expansionMuseum specimensGambiae complexGambiaeSpeciesAnopheles arabiensisIdentityDNA analysis
2007
Genetic population structure of Anopheles gambiae in Equatorial Guinea
Moreno M, Salgueiro P, Vicente J, Cano J, Berzosa P, de Lucio A, Simard F, Caccone A, Do Rosario V, Pinto J, Benito A. Genetic population structure of Anopheles gambiae in Equatorial Guinea. Malaria Journal 2007, 6: 137. PMID: 17937805, PMCID: PMC2100067, DOI: 10.1186/1475-2875-6-137.Peer-Reviewed Original ResearchConceptsGenetic differentiationMicrosatellite lociPopulation structureMainland samplesGenetic diversity parametersGenetic population structureGambiae s.A. gambiae s.Incipient speciationPopulation differentiationGene flowGenetic structureGenetic isolationS formMicrosatellite dataGenomic islandsContinental Equatorial GuineaDiversity parametersMosquito vector populationsChromosome 3Anopheles gambiaeIsland samplesAnopheles gambiae s.Shallow differentiationIsland of BiokoPatterns of Selection in Anti-Malarial Immune Genes in Malaria Vectors: Evidence for Adaptive Evolution in LRIM1 in Anopheles arabiensis
Slotman MA, Parmakelis A, Marshall JC, Awono-Ambene PH, Antonio-Nkondjo C, Simard F, Caccone A, Powell JR. Patterns of Selection in Anti-Malarial Immune Genes in Malaria Vectors: Evidence for Adaptive Evolution in LRIM1 in Anopheles arabiensis. PLOS ONE 2007, 2: e793. PMID: 17726523, PMCID: PMC1945087, DOI: 10.1371/journal.pone.0000793.Peer-Reviewed Original ResearchConceptsAdaptive evolutionPrimary malaria vectorMalaria vectorsPatterns of selectionNon-vector speciesAnopheles gambiae complexMcDonald-KreitmanReplacement substitutionsPositive selectionGenetic variationMalaria vector AnLRIM1Immune genesSelection showGambiae complexGenesAdjacent codonsProtein differentiationLevel of resistanceAdaptive responsePlasmodium speciesCEC1Vector AnMaximum likelihood testSpecies
2006
Genetic differentiation between the BAMAKO and SAVANNA chromosomal forms of Anopheles gambiae as indicated by amplified fragment length polymorphism analysis.
Slotman M, Mendez M, Torre A, Dolo G, Touré Y, Caccone A. Genetic differentiation between the BAMAKO and SAVANNA chromosomal forms of Anopheles gambiae as indicated by amplified fragment length polymorphism analysis. American Journal Of Tropical Medicine And Hygiene 2006, 74: 641-8. PMID: 16606999, DOI: 10.4269/ajtmh.2006.74.641.Peer-Reviewed Original ResearchConceptsChromosomal formsAnopheles gambiaeSavanna chromosomal formsRibosomal DNA locusFragment length polymorphism (AFLP) primer combinationsPolymorphism primer combinationsGenetic differentiationGene flowDNA lociAFLP analysisFragment length polymorphism analysisPrimer combinationsMolecular markersDiagnostic differencesRecent originLength polymorphism analysisSavannaGambiaePolymorphism analysisMain vectorSympatryGenomeLociDifferentiationMopti