2020
Mitomycin-C treatment during differentiation of induced pluripotent stem cell-derived dopamine neurons reduces proliferation without compromising survival or function in vivo
Hiller B, Marmion D, Gross R, Thompson C, Chavez C, Brundin P, Wakeman D, McMahon C, Kordower J. Mitomycin-C treatment during differentiation of induced pluripotent stem cell-derived dopamine neurons reduces proliferation without compromising survival or function in vivo. Stem Cells Translational Medicine 2020, 10: 278-290. PMID: 32997443, PMCID: PMC7848297, DOI: 10.1002/sctm.20-0014.Peer-Reviewed Original ResearchConceptsDopamine neuronsInduced pluripotent stem cellsParkinson's diseaseStem cell-derived dopamine neuronsPD cell therapyMidbrain dopamine neuronsLong-term survivalTransplant of cellsStem cellsHuman induced pluripotent stem cellsPluripotent stem cellsNeuron preparationsMitomycin C treatmentAthymic ratsDrug selectionUndesirable proliferationCell therapyRobust survivalLower proliferationVivo functionNeuronsTransplantationSurvivalProliferative cellsDisease
2017
Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo
Wakeman D, Hiller B, Marmion D, McMahon C, Corbett G, Mangan K, Ma J, Little L, Xie Z, Perez-Rosello T, Guzman J, Surmeier D, Kordower J. Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo. Stem Cell Reports 2017, 9: 149-161. PMID: 28579395, PMCID: PMC5511045, DOI: 10.1016/j.stemcr.2017.04.033.Peer-Reviewed Original ResearchConceptsParkinson's diseaseDopamine neuronsMidbrain dopamine neuronsStem cell therapyGrafted neuronsHost striatumCell-based therapiesPluripotent stem cell therapyFunctional deficitsPrimate modelFiber innervationParkinsonian phenotypeTherapeutic efficacyTransplantation studiesCell therapyNeuronsSignificant reversalTranslational developmentBehavioral assessmentClinical applicationTherapyElectrophysiological signaturesRatsDiseaseMinimal manipulation
2015
Autologous iPSC‐derived dopamine neuron grafts show considerable promise in a nonhuman primate model of Parkinson's disease
Wakeman D. Autologous iPSC‐derived dopamine neuron grafts show considerable promise in a nonhuman primate model of Parkinson's disease. Movement Disorders 2015, 30: 1034-1034. PMID: 26095814, DOI: 10.1002/mds.26267.Peer-Reviewed Original Research
2014
Survival and Integration of Neurons Derived from Human Embryonic Stem Cells in MPTP-Lesioned Primates
Wakeman DR, Weiss S, Sladek JR, Elsworth JD, Bauereis B, Leranth C, Hurley PJ, Roth RH, Redmond DE. Survival and Integration of Neurons Derived from Human Embryonic Stem Cells in MPTP-Lesioned Primates. Cell Transplantation 2014, 23: 981-994. PMID: 23562290, DOI: 10.3727/096368913x664865.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cell linesEmbryonic stem cell linesHuman embryonic stem cellsEmbryonic stem cellsGene expression studiesStem cell linesGFP lentiviral vectorExpression studiesDifferentiated cellsDifferentiation protocolsDopamine neuronal survivalIntegration of neuronsNeuronal cellsNeuronal phenotypeTyrosine hydroxylaseStem cellsExtension of processesBiochemical analysisDopaminergic marker tyrosine hydroxylaseHESCCell linesIII-tubulinMidbrain of MPTPPhenotypeMembrane depolarizationPeripheral alpha‐synuclein and Parkinson's disease
Olanow C, Wakeman D, Kordower J. Peripheral alpha‐synuclein and Parkinson's disease. Movement Disorders 2014, 29: 963-966. PMID: 25043799, DOI: 10.1002/mds.25966.Peer-Reviewed Original Research
2011
Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson’s disease
Kriks S, Shim J, Piao J, Ganat Y, Wakeman D, Xie Z, Carrillo-Reid L, Auyeung G, Antonacci C, Buch A, Yang L, Beal M, Surmeier D, Kordower J, Tabar V, Studer L. Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson’s disease. Nature 2011, 480: 547-551. PMID: 22056989, PMCID: PMC3245796, DOI: 10.1038/nature10648.Peer-Reviewed Original ResearchCell Transplantation and Gene Therapy in Parkinson's Disease
Wakeman D, Dodiya H, Kordower J. Cell Transplantation and Gene Therapy in Parkinson's Disease. Annals Of Global Health 2011, 78: 126-158. PMID: 21259269, DOI: 10.1002/msj.20233.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell TransplantationGene Transfer TechniquesHumansNeuronsParkinson DiseaseStem Cell TransplantationConceptsParkinson's diseaseLong-term disease modificationFetal dopamine neuronsGraft-induced dyskinesiaStem cell transplantationCourse of diseaseDirect cell replacementProgressive neurodegenerative disorderWidespread clinical useGene therapyPatient-derived cellsDopamine replacementMotor symptomsDisease modificationDopamine deficiencyViral vector deliveryCell transplantationSymptomatic strategiesDopamine neuronsClinical trialsEfficacy profileDopaminergic circuitryMotor neuronsClinical studiesVentral midbrain
2007
Behavioral improvement in a primate Parkinson's model is associated with multiple homeostatic effects of human neural stem cells
Redmond DE, Bjugstad KB, Teng YD, Ourednik V, Ourednik J, Wakeman DR, Parsons XH, Gonzalez R, Blanchard BC, Kim SU, Gu Z, Lipton SA, Markakis EA, Roth RH, Elsworth JD, Sladek JR, Sidman RL, Snyder EY. Behavioral improvement in a primate Parkinson's model is associated with multiple homeostatic effects of human neural stem cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 12175-12180. PMID: 17586681, PMCID: PMC1896134, DOI: 10.1073/pnas.0704091104.Peer-Reviewed Original ResearchConceptsHuman neural stem cellsSubstantia nigraParkinson's diseaseNeural stem cellsTyrosine hydroxylaseBehavioral improvementModel of PDHost substantia nigraStem cellsResponsive progenitor cellsAlpha-synuclein aggregationDA markersNigrostriatal circuitryParkinsonian primatesParkinsonian signsNeuronal numberDA levelsFunctional improvementParkinson modelImmunopositive cellsNormalizing effectDA phenotypeHomeostatic effectsNumber of diseasesProgenitor cells
2006
Large animal models are critical for rationally advancing regenerative therapies
Wakeman D, Crain A, Snyder E. Large animal models are critical for rationally advancing regenerative therapies. Regenerative Medicine 2006, 1: 405-413. PMID: 17465832, PMCID: PMC2905042, DOI: 10.2217/17460751.1.4.405.Peer-Reviewed Original Research