2014
Survival and Integration of Neurons Derived from Human Embryonic Stem Cells in MPTP-Lesioned Primates
Wakeman DR, Weiss S, Sladek JR, Elsworth JD, Bauereis B, Leranth C, Hurley PJ, Roth RH, Redmond DE. Survival and Integration of Neurons Derived from Human Embryonic Stem Cells in MPTP-Lesioned Primates. Cell Transplantation 2014, 23: 981-994. PMID: 23562290, DOI: 10.3727/096368913x664865.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cell linesEmbryonic stem cell linesHuman embryonic stem cellsEmbryonic stem cellsGene expression studiesStem cell linesGFP lentiviral vectorExpression studiesDifferentiated cellsDifferentiation protocolsDopamine neuronal survivalIntegration of neuronsNeuronal cellsNeuronal phenotypeTyrosine hydroxylaseStem cellsExtension of processesBiochemical analysisDopaminergic marker tyrosine hydroxylaseHESCCell linesIII-tubulinMidbrain of MPTPPhenotypeMembrane depolarizationNeonatal immune-tolerance in mice does not prevent xenograft rejection
Mattis V, Wakeman D, Tom C, Dodiya H, Yeung S, Tran A, Bernau K, Ornelas L, Sahabian A, Reidling J, Sareen D, Thompson L, Kordower J, Svendsen C. Neonatal immune-tolerance in mice does not prevent xenograft rejection. Experimental Neurology 2014, 254: 90-98. PMID: 24440640, PMCID: PMC3954854, DOI: 10.1016/j.expneurol.2014.01.007.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAnimals, Outbred StrainsCells, CulturedCorpus StriatumDisease Models, AnimalFemaleGraft RejectionGraft SurvivalHeterograftsHumansHuntingtin ProteinHuntington DiseaseImmune ToleranceMaleMiceMice, Inbred NODMice, SCIDMice, TransgenicNerve Tissue ProteinsNeural Stem CellsNuclear ProteinsTransplantation, HeterologousConceptsNeonatal toleranceRapid rejectionHuman stem cell transplantationImmune-intact miceSignificant immune rejectionStem cell transplantationHumanized mouse modelXenograft rejectionCell transplantationRodent modelsMouse modelImmune rejectionMiceConflicting resultsStem cell typesXenograftsRecent reportsCell typesRejectionTransplantationRatsDisease
2007
Behavioral improvement in a primate Parkinson's model is associated with multiple homeostatic effects of human neural stem cells
Redmond DE, Bjugstad KB, Teng YD, Ourednik V, Ourednik J, Wakeman DR, Parsons XH, Gonzalez R, Blanchard BC, Kim SU, Gu Z, Lipton SA, Markakis EA, Roth RH, Elsworth JD, Sladek JR, Sidman RL, Snyder EY. Behavioral improvement in a primate Parkinson's model is associated with multiple homeostatic effects of human neural stem cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 12175-12180. PMID: 17586681, PMCID: PMC1896134, DOI: 10.1073/pnas.0704091104.Peer-Reviewed Original ResearchConceptsHuman neural stem cellsSubstantia nigraParkinson's diseaseNeural stem cellsTyrosine hydroxylaseBehavioral improvementModel of PDHost substantia nigraStem cellsResponsive progenitor cellsAlpha-synuclein aggregationDA markersNigrostriatal circuitryParkinsonian primatesParkinsonian signsNeuronal numberDA levelsFunctional improvementParkinson modelImmunopositive cellsNormalizing effectDA phenotypeHomeostatic effectsNumber of diseasesProgenitor cells