2014
Survival and Integration of Neurons Derived from Human Embryonic Stem Cells in MPTP-Lesioned Primates
Wakeman DR, Weiss S, Sladek JR, Elsworth JD, Bauereis B, Leranth C, Hurley PJ, Roth RH, Redmond DE. Survival and Integration of Neurons Derived from Human Embryonic Stem Cells in MPTP-Lesioned Primates. Cell Transplantation 2014, 23: 981-994. PMID: 23562290, DOI: 10.3727/096368913x664865.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cell linesEmbryonic stem cell linesHuman embryonic stem cellsEmbryonic stem cellsGene expression studiesStem cell linesGFP lentiviral vectorExpression studiesDifferentiated cellsDifferentiation protocolsDopamine neuronal survivalIntegration of neuronsNeuronal cellsNeuronal phenotypeTyrosine hydroxylaseStem cellsExtension of processesBiochemical analysisDopaminergic marker tyrosine hydroxylaseHESCCell linesIII-tubulinMidbrain of MPTPPhenotypeMembrane depolarization
2011
Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson’s disease
Kriks S, Shim J, Piao J, Ganat Y, Wakeman D, Xie Z, Carrillo-Reid L, Auyeung G, Antonacci C, Buch A, Yang L, Beal M, Surmeier D, Kordower J, Tabar V, Studer L. Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson’s disease. Nature 2011, 480: 547-551. PMID: 22056989, PMCID: PMC3245796, DOI: 10.1038/nature10648.Peer-Reviewed Original Research
2010
Cellular Repair in the Parkinsonian Nonhuman Primate Brain
Redmond DE, Weiss S, Elsworth JD, Roth RH, Wakeman DR, Bjugstad KB, Collier TJ, Blanchard BC, Teng YD, Synder EY, Sladek JR. Cellular Repair in the Parkinsonian Nonhuman Primate Brain. Rejuvenation Research 2010, 13: 188-194. PMID: 20370501, PMCID: PMC2946058, DOI: 10.1089/rej.2009.0960.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainDopamineEmbryonic Stem CellsHumansNerve RegenerationNeuronsParkinsonian DisordersPrimatesStem Cell TransplantationConceptsHuman neural stem cellsSubstantia nigraDopamine neuronsParkinson's diseaseFetal dopaminergic neuronsFetal striatal tissueReduction of medicationLittle therapeutic benefitParkinsonian motor deficitsSubsequent clinical trialsDopaminergic neural systemsNonhuman primate brainCell replacement strategiesLong-term improvementStem cellsNeural stem cellsMotor deficitsDopaminergic neuronsNeurological signsCellular repairClinical trialsStriatal targetsStriatal tissueTherapeutic benefitParkinson's patients
2009
Long‐Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells
Wakeman DR, Hofmann MR, Redmond DE, Teng YD, Snyder EY. Long‐Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells. Current Protocols In Stem Cell Biology 2009, 9: 2d.3.1-2d.3.77. PMID: 19455542, DOI: 10.1002/9780470151808.sc02d03s9.Peer-Reviewed Original ResearchConceptsCentral nervous system disordersNSC/NPCsNeural stem/precursor cellsNeural stem/progenitor cellsNervous system disordersEarly human neurogenesisStem/precursor cellsSmall molecule drug screeningStem/progenitor cellsForebrain neural stem cellsNeural stem cellsHuman neural stem/precursor cellsAdult rodentsTherapeutic modalitiesParkinson's diseaseSystem disordersCadaveric sourcesMonkey brainAlzheimer's diseaseNeurodegenerative disordersHuman neurogenesisNeuronal migrationDiseaseLong-term maintenanceMigratory capability
2006
Adhesive Interactions Between Human Neural Stem Cells and Inflamed Human Vascular Endothelium Are Mediated by Integrins
Mueller F, Serobyan N, Schraufstatter I, DiScipio R, Wakeman D, Loring J, Snyder E, Khaldoyanidi S. Adhesive Interactions Between Human Neural Stem Cells and Inflamed Human Vascular Endothelium Are Mediated by Integrins. Stem Cells 2006, 24: 2367-2372. PMID: 17071855, PMCID: PMC2885956, DOI: 10.1634/stemcells.2005-0568.Peer-Reviewed Original ResearchMeSH KeywordsCell AdhesionCell LineCell MovementEmbryonic Stem CellsEndothelial CellsHumansImmunohistochemistryInflammationIntegrin alpha2Integrin alpha6Integrin beta1IntegrinsNeuronsReverse Transcriptase Polymerase Chain ReactionStress, MechanicalTumor Necrosis Factor-alphaUp-RegulationVascular Cell Adhesion Molecule-1