2020
A Biomarker for Predicting Responsiveness to Stem Cell Therapy Based on Mechanism-of-Action: Evidence from Cerebral Injury
Hartman R, Nathan N, Ghosh N, Pernia C, Law J, Nuryyev R, Plaia A, Yusof A, Tone B, Dulcich M, Wakeman D, Dilmac N, Niles W, Sidman R, Obenaus A, Snyder E, Ashwal S. A Biomarker for Predicting Responsiveness to Stem Cell Therapy Based on Mechanism-of-Action: Evidence from Cerebral Injury. Cell Reports 2020, 31: 107622. PMID: 32402283, DOI: 10.1016/j.celrep.2020.107622.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersBrain InjuriesDisease Models, AnimalRatsRats, Sprague-DawleyRegenerative MedicineStem Cell TransplantationConceptsHuman neural stem cellsHypoxic-ischemic injuryTherapeutic mechanismCerebral hypoxic-ischemic injuryCell therapyMolecular profileHierarchical region splittingStem cell therapyNeural stem cellsCerebral injurySelection biomarkerPredicting ResponsivenessNecrotic coreCognitive outcomesBiomarkersTherapyInjuryLesionsStem cellsResponsivenessPenumbraCellsCore volumeSalvageabilityRecipients
2017
Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo
Wakeman D, Hiller B, Marmion D, McMahon C, Corbett G, Mangan K, Ma J, Little L, Xie Z, Perez-Rosello T, Guzman J, Surmeier D, Kordower J. Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo. Stem Cell Reports 2017, 9: 149-161. PMID: 28579395, PMCID: PMC5511045, DOI: 10.1016/j.stemcr.2017.04.033.Peer-Reviewed Original ResearchConceptsParkinson's diseaseDopamine neuronsMidbrain dopamine neuronsStem cell therapyGrafted neuronsHost striatumCell-based therapiesPluripotent stem cell therapyFunctional deficitsPrimate modelFiber innervationParkinsonian phenotypeTherapeutic efficacyTransplantation studiesCell therapyNeuronsSignificant reversalTranslational developmentBehavioral assessmentClinical applicationTherapyElectrophysiological signaturesRatsDiseaseMinimal manipulation
2014
Homing of Neural Stem Cells From the Venous Compartment Into a Brain Infarct Does Not Involve Conventional Interactions With Vascular Endothelium
Goncharova V, Das S, Niles W, Schraufstatter I, Wong A, Povaly T, Wakeman D, Miller L, Snyder E, Khaldoyanidi S. Homing of Neural Stem Cells From the Venous Compartment Into a Brain Infarct Does Not Involve Conventional Interactions With Vascular Endothelium. Stem Cells Translational Medicine 2014, 3: 229-240. PMID: 24396034, PMCID: PMC3925049, DOI: 10.5966/sctm.2013-0052.Peer-Reviewed Original ResearchConceptsHuman neural stem cellsNeural stem cellsIntravenous administrationStromal cell-derived factor-1αEx vivo fucosylationCutaneous lymphocyte antigenBlood-brain barrierStem cellsMortality of ratsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsImplantation of cellsBrain infarctsVein endothelial cellsLymphocyte antigenVascular endotheliumIntracranial injectionVenous compartmentCell surface moietiesFactor-1αIntravascular spaceEndothelial cellsAdministrationLuminal surfaceRemoval of fucose
2011
Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson’s disease
Kriks S, Shim J, Piao J, Ganat Y, Wakeman D, Xie Z, Carrillo-Reid L, Auyeung G, Antonacci C, Buch A, Yang L, Beal M, Surmeier D, Kordower J, Tabar V, Studer L. Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson’s disease. Nature 2011, 480: 547-551. PMID: 22056989, PMCID: PMC3245796, DOI: 10.1038/nature10648.Peer-Reviewed Original Research