2024
Pathophysiology and Prevention of Manual-Ventilation-Induced Lung Injury (MVILI)
White L, Conrad S, Alexander J. Pathophysiology and Prevention of Manual-Ventilation-Induced Lung Injury (MVILI). Pathophysiology 2024, 31: 583-595. PMID: 39449524, PMCID: PMC11503381, DOI: 10.3390/pathophysiology31040042.Peer-Reviewed Original ResearchManual ventilationLung injuryManual hyperventilationOperator-dependent skillsShort-term ventilationMechanism of injuryManikin-based studyBag valve maskAmerican Heart AssociationEuropean Resuscitation CouncilHyperventilated patientsMechanical ventilationHemodynamic alterationsHealthcare providersHeart AssociationPatient populationResuscitation effortsGastric regurgitationResuscitation CouncilCardiopulmonary resuscitationVentilation parametersPatientsInjury processAcute barotraumaVentilationEnhanced Manual Ventilation with a Handheld Audiovisual Device - BENGI - Insights from a Pilot Study in Special Operations Medicine.
White L, Maxey B, Solitro G, Conrad S, Davidson K, Alhaque A, Alexander J. Enhanced Manual Ventilation with a Handheld Audiovisual Device - BENGI - Insights from a Pilot Study in Special Operations Medicine. Journal Of Special Operations Medicine 2024, 24: 9-17. PMID: 39285506, DOI: 10.55460/76gq-gsck.Peer-Reviewed Original ResearchImpaired Peripheral Vascular Function Following Ischemic Stroke in Mice: Potential Insights into Blood Pressure Variations in the Post-Stroke Patient
Yilmaz G, Alexander J. Impaired Peripheral Vascular Function Following Ischemic Stroke in Mice: Potential Insights into Blood Pressure Variations in the Post-Stroke Patient. Pathophysiology 2024, 31: 488-501. PMID: 39311310, PMCID: PMC11417821, DOI: 10.3390/pathophysiology31030036.Peer-Reviewed Original ResearchIncreased blood pressure variabilityBlood pressure variabilityIschemic strokeAcetylcholine-induced endothelium-dependent vasodilationAssociated with poor clinical outcomesEndothelium-dependent vasodilator responsesPeripheral arteriesVascular smooth muscle toneResponse to SNPStroke animalsImpaired peripheral vascular functionPhenylephrine-induced vasoconstrictionEndothelium-dependent vasodilationPoor clinical outcomesSmooth muscle toneThoracic aortic segmentsDeterminants of blood pressurePressure variabilityPeripheral vascular functionPeripheral vascular responseWire myographyIsolated aortaVasoconstrictor responsesVasodilator responseRemote arterySTABILITY (Symptomatic Review during Biologic Therapy) of Inflammatory Bowel Disease Patients Receiving Infusion Therapy Improves Clinical Outcomes
Morgan K, Morris J, Cai Q, Kilgore P, Cvek U, Trutschl M, Lofton K, Mavuram M, Ramesh P, Dao N, Alhaque A, Alexander J. STABILITY (Symptomatic Review during Biologic Therapy) of Inflammatory Bowel Disease Patients Receiving Infusion Therapy Improves Clinical Outcomes. Pathophysiology 2024, 31: 398-407. PMID: 39189166, PMCID: PMC11348379, DOI: 10.3390/pathophysiology31030030.Peer-Reviewed Original ResearchInflammatory bowel disease patientsInflammatory bowel diseaseFemale IBD patientsMale CD patientsMale IBD patientsCD patientsUC patientsTherapy visitsIBD patientsDisease severityInfusion therapyFemale UC patientsMale UC patientsRetrospective chart reviewIBD-related symptomsPhysician-patient contactLow disease activityPhysician-patient interviewImprove patient outcomesFemale CD patientsCare gapsImproving clinical outcomesDiagnosis dateMedication changesChronic diseasesInhibition of GSK3α,β rescues cognitive phenotypes in a preclinical mouse model of CTNNB1 syndrome
Alexander J, Vazquez-Ramirez L, Lin C, Antonoudiou P, Maguire J, Wagner F, Jacob M. Inhibition of GSK3α,β rescues cognitive phenotypes in a preclinical mouse model of CTNNB1 syndrome. EMBO Molecular Medicine 2024, 16: 2109-2131. PMID: 39103699, PMCID: PMC11393422, DOI: 10.1038/s44321-024-00110-5.Peer-Reviewed Original ResearchHeterozygous loss-of-function variantsLoss-of-function variantsWnt signal transductionWnt target gene expressionSynaptic adhesion complexesTarget gene expressionB-cateninCognitive phenotypesHeterozygous mouse linePreclinical mouse modelsWild-type littermatesBrain functionAdhesion complexesSignal transductionEfficacious treatmentNeuronal functional propertiesRegulation of changesGene expressionMotor deficitsHeterozygous miceN-cadherinNa/K-ATPaseBrainMotor disabilityMouse modelDistraction Enterogenesis in Rats: A Novel Approach for the Treatment of Short Bowel Syndrome
O’Quin C, Clayton S, Trosclair L, Meyer H, Dao N, Minagar A, White L, Welch V, Solitro G, Alexander J, Sorrells D. Distraction Enterogenesis in Rats: A Novel Approach for the Treatment of Short Bowel Syndrome. Pathophysiology 2024, 31: 388-397. PMID: 39189165, PMCID: PMC11348226, DOI: 10.3390/pathophysiology31030029.Peer-Reviewed Original ResearchTreatment of short bowel syndromeIn vivo rat studyRat intestinal modelShort bowel syndromeSprague Dawley ratsSmall intestineParenteral nutritionBowel histologyBowel lengthDawley ratsLength-tension relationshipMucosal erosionsRat small intestineRat studiesBowel syndromeEx vivoRatsBowel tissueFailure loadPost-deploymentPatientsBowelHistologyLoad test dataIntestinal modelHistologic Analysis of ‘Distraction Vaginogenesis’ in a Rat Model
Meyer H, Trosclair L, Clayton S, O’Quin C, Crochet C, Colvin J, Welch V, Alhaque A, Solitro G, Shah-Bruce M, Alexander J, Sorrells D. Histologic Analysis of ‘Distraction Vaginogenesis’ in a Rat Model. Pathophysiology 2024, 31: 298-308. PMID: 38921727, PMCID: PMC11206798, DOI: 10.3390/pathophysiology31020022.Peer-Reviewed Original ResearchVaginal agenesisStained with hematoxylin and eosin (H&ERat vaginal tissueSprague Dawley ratsLocal immune reactionHematoxylin and eosin (H&EParaffin-embedded tissuesMasson's trichrome stainingAnti-desmin antibodiesMullerian agenesisResin capEosin (H&EVaginal tissueSurgical vaginoplastyVaginal canalDesmin immunostainingCanal lengthSubmucosal eosinophiliaMechanical dilatationNeutrophilic inflammationPost-surgicalDawley ratsChronic inflammationRat modelTrichrome stainingN-Methyl-D-aspartic acid (NMDA) mediated vasodilation in aorta
Yilmaz G, Alexander J. N-Methyl-D-aspartic acid (NMDA) mediated vasodilation in aorta. Physiology 2024, 39: 2323. DOI: 10.1152/physiol.2024.39.s1.2323.Peer-Reviewed Original ResearchN-methyl-D-aspartic acidNitric oxide synthaseVascular smooth muscleVascular responsesSmooth muscleN-methyl-D-aspartic acid receptor blockadeResponse to NMDAWild type male miceNMDA-induced vasodilationNon-NMDA receptorsIn vitro myographyThoracic aorta segmentsPeripheral vascular responseMK-801Receptor blockadeNMDA concentrationsNMDA receptorsResistance arteriesEndothelial cell layerL-NAMEOsmolarity solutionVasodilatory effectMethyl ester hydrochlorideAortic segmentsInduce vasodilationDevelopment of the Cardiovascular Undergraduate Research Initiative for Underrepresented Students (CURIOUS)
Stokes K, Alexander J, Rodgers K, Woolard M. Development of the Cardiovascular Undergraduate Research Initiative for Underrepresented Students (CURIOUS). Physiology 2024, 39: 2076. DOI: 10.1152/physiol.2024.39.s1.2076.Peer-Reviewed Original ResearchState-of-the-art techniquesState-of-the-artSchool of Graduate StudiesFirst-year studentsCardiovascular researchSummer research programEnd-of-programUndergraduate research initiativesPeer review processHTML formatIntensive experimentsProfessional developmentUnderrepresented studentsStudents' considerationUndergraduate programsGraduate educationStudentsPresentation skillsCareer goalsGraduate studiesGraduate collegeUndergraduate studentsNIH National Heart125. The Retention of Significant Vaginal Lengthening with a Novel Vaginal Expansion Device
Colvin J, Meyer H, O'Quin C, Welch V, Solitro G, Shah-Bruce M, Alexander J, Sorrells D. 125. The Retention of Significant Vaginal Lengthening with a Novel Vaginal Expansion Device. Journal Of Pediatric And Adolescent Gynecology 2024, 37: 300. DOI: 10.1016/j.jpag.2024.01.132.Peer-Reviewed Original ResearchVaginal tissueVaginal canalCanal lengthVaginal lengthVaginal dilator therapyPost removalMullerian agenesisResin capVaginal atresiaWeeks of recoveryDilation therapySurgical vaginoplastyCongenital absenceWeeks post-removalChronic inflammationRe-implantationHistological examinationHistological changesHistological analysisTissue contractionWeeksCanalTissueVaginoplastyAgenesis
2017
Mechanisms underlying hPMSC‐based protection on human brain endothelial cells
Barzegar M, Minagar A, Wang Y, Yun J, Boyer C, Couraud P, Alexander J. Mechanisms underlying hPMSC‐based protection on human brain endothelial cells. The FASEB Journal 2017, 31 DOI: 10.1096/fasebj.31.1_supplement.lb704.Peer-Reviewed Original ResearchHuman brain vascular smooth muscle cellsHuman brain endothelial cellsBlood-brain barrierBrain endothelial cellsBrain vascular smooth muscle cellsPlacental-derived mesenchymal stem cellsBrain ischemia/reperfusionEndothelial cellsPro-inflammatory mediatorsImmune cell infiltrationLong-term disabilityVascular smooth muscle cellsIschemia/reperfusionLocal pro-inflammatory mediatorsStroke-affected brainClaudin-1 proteinSmooth muscle cellsCollagen gel contractionStem cellsFITC-dextran permeabilityMatrigel basement membrane matrixStroke injuryVascular reactivityCell-based therapiesChronic phase
2016
Brain Endothelial Cells Segregate Lymphatic‐Venous Biomarkers into Microparticles Following Inflammatory Cytokine Stimulation
Yun J, Minagar A, Tsunoda I, Alexander J. Brain Endothelial Cells Segregate Lymphatic‐Venous Biomarkers into Microparticles Following Inflammatory Cytokine Stimulation. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.993.9.Peer-Reviewed Original ResearchBrain endothelial cellsMultiple sclerosisPro-inflammatory cytokines tumor necrosis factorEndothelial cellsCytokines tumor necrosis factorLymphatic vessel endothelial hyaluronan receptorElevated inflammatory cytokinesLYVE-1Department of NeurologyPro-inflammatory cytokinesSignificant decreaseTumor necrosis factorClearance of fluidIFN-γ treatmentVascular endothelial growth factor receptor 3Growth factor receptor 3Forkhead box C2Human brain tissueInflammatory cytokine stimulationEndothelial disturbanceEndothelial microparticlesInflammatory cytokinesImmune cellsNecrosis factorProspero homeobox 1
2003
Interferon (IFN)-ß1a and IFN-ß1b Block IFN-?-Induced Disintegration of Endothelial Junction Integrity and Barrier
Minagar A, Long A, Ma T, Jackson T, Kelley R, Ostanin D, Sasaki M, Warren A, Jawahar A, Cappell B, Alexander J. Interferon (IFN)-ß1a and IFN-ß1b Block IFN-?-Induced Disintegration of Endothelial Junction Integrity and Barrier. Endothelium 2003, 10: 299-307. PMID: 14741845, DOI: 10.1080/10623320390272299.Peer-Reviewed Original ResearchConceptsIFN beta-1aIFN-beta-1bEffect of IFN beta-1bEffect of IFN-gammaIFN-gammaHuman umbilical vein endothelial cellsIFN-betaVE-cadherin expressionVE-cadherinEffects of IFN beta-1aMultiple sclerosisJunction proteinsIFN-gamma-induced decreaseEndothelial cellsEffect of IFN-betaRelapsing-remitting multiple sclerosisEndothelial junctional proteinsEndothelial tight junctionsProtective effectIFN-gamma effectPathogenesis of MSEndothelial junctional integrityInflammatory cell migrationBlood-brain barrierVascular endothelial-cadherin
2002
Mild hypothermia induced before cardiac arrest reduces brain edema formation in rats.
Xiao F, Zhang S, Arnold T, Alexander J, Huang J, Carden D, Conrad S. Mild hypothermia induced before cardiac arrest reduces brain edema formation in rats. Academic Emergency Medicine 2002, 9: 105-14. PMID: 11825833, DOI: 10.1111/j.1553-2712.2002.tb00224.x.Peer-Reviewed Original ResearchConceptsCerebral spinal fluid pressureWet-to-dry weight ratioBrain wet-to-dry weight ratioBrain edema formationMild hypothermiaGroup 2Group 1Cardiac arrestEdema formationGroup 3Spinal fluid pressureMinutes of asphyxiationCell junction proteinsEndothelial cell junction proteinsOccludin degradationBrain myeloperoxidaseMm HgControl surgeryBrain edemaJunction proteinsNormothermic CANeutrophil contentHypothermiaWestern blottingBrain damage
2001
Reciprocal regulation of endothelial substrate adhesion and barrier function.
Alexander J, Zhu Y, Elrod J, Alexander B, Coe L, Kalogeris T, Fuseler J. Reciprocal regulation of endothelial substrate adhesion and barrier function. Microcirculation 2001, 8: 389-401. PMID: 11781812, DOI: 10.1038/sj/mn/7800111.Peer-Reviewed Original ResearchConceptsCell-substrate adhesionFocal adhesion protein paxillinDecreased endothelial barrier functionEnhanced substrate bindingCell-cell contactProtein paxillinRegulation in vitroSubstrate bindingTyrosine phosphorylationTreated with test agentsExposure to inflammatory mediatorsProtein phosphorylationMicrovascular endothelial monolayersMM)- and timeBarrier functionReciprocal regulationCell adhesionCytoplasmic Ca2+Substrate adhesionEndothelial barrier functionTreatment of monolayersPromote localizationRemoval of H2O2ProteinPKG inhibition