2024
Don’t be so naïve
Horsley V, Nassereddine A. Don’t be so naïve. ELife 2024, 13: e103292. PMID: 39453398, PMCID: PMC11509665, DOI: 10.7554/elife.103292.Peer-Reviewed Original ResearchMeSH KeywordsActinsCell DifferentiationEmbryonic Stem CellsHuman Embryonic Stem CellsHumansPluripotent Stem Cells
2015
Edges of human embryonic stem cell colonies display distinct mechanical properties and differentiation potential
Rosowski KA, Mertz AF, Norcross S, Dufresne ER, Horsley V. Edges of human embryonic stem cell colonies display distinct mechanical properties and differentiation potential. Scientific Reports 2015, 5: 14218. PMID: 26391588, PMCID: PMC4585749, DOI: 10.1038/srep14218.Peer-Reviewed Original ResearchMeSH KeywordsCell DifferentiationColony-Forming Units AssayCytoskeletonHuman Embryonic Stem CellsHumansMechanical PhenomenaConceptsHuman embryonic stem cellsUndifferentiated coloniesDifferentiation processCell fate decisionsHuman embryonic stem cell coloniesEmbryonic stem cell coloniesEmbryonic stem cellsEdges of coloniesStrong traction forcesStem cell coloniesEarly human developmentDevelopmental decisionsFate decisionsActin organizationLive imagingColony geometryMyosin activityCellular environmentSmall coloniesAdherent coloniesDifferentiation potentialCell coloniesDifferentiation efficiencyStem cellsColoniesTranscriptional Profiling of Ectoderm Specification to Keratinocyte Fate in Human Embryonic Stem Cells
Tadeu AM, Lin S, Hou L, Chung L, Zhong M, Zhao H, Horsley V. Transcriptional Profiling of Ectoderm Specification to Keratinocyte Fate in Human Embryonic Stem Cells. PLOS ONE 2015, 10: e0122493. PMID: 25849374, PMCID: PMC4388500, DOI: 10.1371/journal.pone.0122493.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsEmbryonic stem cellsEctoderm specificationStem cellsHuman embryonic stem cell differentiationEmbryonic stem cell differentiationStem cell differentiationKeratinocyte fateEctoderm lineageEpidermal specificationTranscriptional regulationCandidate regulatorsTranscriptional profilingEpidermal developmentGrowth factor activityProtein aP2Keratinocyte developmentCell differentiationΓ-secretase inhibitor DAPTGenesFactor activityHomeostatic conditionsEpithelial tissuesInhibitor DAPTCell signature