2023
Pooled screening with next-generation gene editing tools
Zhou L, Yang L, Feng Y, Chen S. Pooled screening with next-generation gene editing tools. Current Opinion In Biomedical Engineering 2023, 28: 100479. PMID: 38222973, PMCID: PMC10786633, DOI: 10.1016/j.cobme.2023.100479.Peer-Reviewed Original ResearchGene editing toolsEditing toolsFunction of genesShort palindromic repeatsPooled screensPalindromic repeatsGenetic elementsPool of cellsBiological processesPooled screeningGenetic variantsPotential targetRecent advancesGenesRepeatsPlantsSequencingMutationsAgricultural researchTherapeutic interventionsSimultaneous examinationExpressionPotential future directionsCellsPool
2022
High-content CRISPR screening
Bock C, Datlinger P, Chardon F, Coelho M, Dong M, Lawson K, Lu T, Maroc L, Norman T, Song B, Stanley G, Chen S, Garnett M, Li W, Moffat J, Qi L, Shapiro R, Shendure J, Weissman J, Zhuang X. High-content CRISPR screening. Nature Reviews Methods Primers 2022, 2: 8. DOI: 10.1038/s43586-021-00093-4.Peer-Reviewed Original ResearchCRISPR screeningCRISPR screensBiological discoverySingle-cell RNA sequencingPooled CRISPR screensList of genesHigh-content methodBiological challengesGene functionCell competitionUnbiased interrogationGuide RNARNA sequencingBioinformatics analysisDetailed biological insightsTarget genesBasic biologyPool of cellsBiological insightsCRISPR technologyMolecular mechanismsSuch screensGenesMedical GeneticsBroad utility