Featured Publications
Endothelial calreticulin deletion impairs endothelial function in aged mice
Biwer L, Askew-Page H, Hong K, Milstein J, Johnstone S, Macal E, Good M, Bagher P, Sonkusare S, Isakson B. Endothelial calreticulin deletion impairs endothelial function in aged mice. AJP Heart And Circulatory Physiology 2020, 318: h1041-h1048. PMID: 32196361, PMCID: PMC7346539, DOI: 10.1152/ajpheart.00586.2019.Peer-Reviewed Original ResearchConceptsVascular functionTauroursodeoxycholic acidMesenteric arteryAged miceCalcium signalsImpaired endothelial-dependent vasodilationThird-order mesenteric arteriesEndothelial cellsEndothelial-dependent vasodilationIncubation of arteriesImpairs endothelial functionSmall resistance arteriesMultifunctional calcium binding proteinER stress inhibitor tauroursodeoxycholic acidWk of ageEndoplasmic reticulum stressPE constrictionEndothelial functionVasodilatory functionResistance arteriesCalcium binding proteinAgonist carbacholClinical trialsMuscarinic stimulationVasodilationNon–Endoplasmic Reticulum–Based Calr (Calreticulin) Can Coordinate Heterocellular Calcium Signaling and Vascular Function
Biwer L, Good M, Hong K, Patel R, Agrawal N, Looft-Wilson R, Sonkusare S, Isakson B. Non–Endoplasmic Reticulum–Based Calr (Calreticulin) Can Coordinate Heterocellular Calcium Signaling and Vascular Function. Arteriosclerosis Thrombosis And Vascular Biology 2017, 38: 120-130. PMID: 29122814, PMCID: PMC5746467, DOI: 10.1161/atvbaha.117.309886.Peer-Reviewed Original ResearchConceptsThird-order mesenteric arteriesBlood pressureMesenteric arteryVascular functionCalcium signalingIEL holesApplication of CChMyoendothelial junctionsCalcium eventsER calciumSmooth muscle cellsInternal elastic laminaVascular reactivityResistance arteriesSmall arteriesSame arteryKnockout miceArteryΔ miceElastic laminaMuscle cellsEndoplasmic reticulum calciumMiceCALRCalcium signals
2016
Two functionally distinct pools of eNOS in endothelium are facilitated by myoendothelial junction lipid composition
Biwer L, Taddeo E, Kenwood B, Hoehn K, Straub A, Isakson B. Two functionally distinct pools of eNOS in endothelium are facilitated by myoendothelial junction lipid composition. Biochimica Et Biophysica Acta 2016, 1861: 671-679. PMID: 27106139, PMCID: PMC4869716, DOI: 10.1016/j.bbalip.2016.04.014.Peer-Reviewed Original ResearchMeSH KeywordsBiological TransportBradykininCalciumCalcium SignalingCell CommunicationCoculture TechniquesCyclic GMPDiglyceridesEgtazic AcidEndoplasmic ReticulumEndothelial CellsGap JunctionsGene Expression RegulationHumansInositol 1,4,5-TrisphosphateMacrocyclic CompoundsMyocytes, Smooth MuscleNitric Oxide Synthase Type IIIOxazolesPhenylephrinePhosphatidylserinesPhosphorylationPrimary Cell CultureProtein Kinase CProtein Kinase InhibitorsThapsigargin