Featured Publications
Loss of Endothelial FTO Antagonizes Obesity-Induced Metabolic and Vascular Dysfunction
Krüger N, Biwer L, Good M, Ruddiman C, Wolpe A, DeLalio L, Murphy S, Macal E, Ragolia L, Serbulea V, Best A, Leitinger N, Harris T, Sonkusare S, Gödecke A, Isakson B. Loss of Endothelial FTO Antagonizes Obesity-Induced Metabolic and Vascular Dysfunction. Circulation Research 2019, 126: 232-242. PMID: 31801409, PMCID: PMC7007767, DOI: 10.1161/circresaha.119.315531.Peer-Reviewed Original ResearchConceptsMyogenic toneProstaglandin DResistance arteriesDeficient miceHigh-fat diet-induced glucose intoleranceDiet-induced glucose intoleranceObesity-induced hypertensionImpact of obesityPrevalence of obesityNew treatment optionsDevelopment of obesityHigh-fat dietRegulation of obesityDifferent cell typesCell typesGlucose intoleranceVascular alterationsVascular changesVascular dysfunctionControl miceInsulin resistanceTreatment optionsCardiovascular diseaseObesityArtery
2017
A Cell Culture Model of Resistance Arteries.
Biwer L, Lechauve C, Vanhoose S, Weiss M, Isakson B. A Cell Culture Model of Resistance Arteries. Journal Of Visualized Experiments 2017 PMID: 28930992, PMCID: PMC5752193, DOI: 10.3791/55992.Peer-Reviewed Original ResearchConceptsCell culture modelCell typesMyoendothelial junctionsEndothelial cell polarizationPlating of cellsIntact cell layerDifferent cell typesSignaling microdomainCulture modelSpecific proteinsCell polarizationPhysiological relevanceActivity assaysProtein isolationSmooth muscle cellsVascular cellsMuscle cellsImmunofluorescent analysisIntact resistance arteriesProteinCellsMuscle fractionsIsolationCell layerVCCC
2014
Regulation of Cellular Communication by Signaling Microdomains in the Blood Vessel Wall
Billaud M, Lohman A, Johnstone S, Biwer L, Mutchler S, Isakson B. Regulation of Cellular Communication by Signaling Microdomains in the Blood Vessel Wall. Pharmacological Reviews 2014, 66: 513-569. PMID: 24671377, PMCID: PMC3973613, DOI: 10.1124/pr.112.007351.Peer-Reviewed Original ResearchConceptsCellular communicationAccumulation of proteinsMechanism of exocytosisSignaling microdomainProper vascular functionPlasma membraneBlood vessel wallCell typesIon channelsMicrodomainsGap junctionsOxygen speciesPotassium channelsVon Willebrand factorPlethora of meansRegulationSpecific regionsEssential componentVessel wall functionWillebrand factorParacrine releaseImportant roleVessel wallExocytosisEndothelium-derived hyperpolarization