2020
Two Distinct Structures of Membrane‐Associated Homodimers of GTP‐ and GDP‐Bound KRAS4B Revealed by Paramagnetic Relaxation Enhancement
Lee K, Fang Z, Enomoto M, Gasmi‐Seabrook G, Zheng L, Koide S, Ikura M, Marshall C. Two Distinct Structures of Membrane‐Associated Homodimers of GTP‐ and GDP‐Bound KRAS4B Revealed by Paramagnetic Relaxation Enhancement. Angewandte Chemie International Edition 2020, 59: 11037-11045. PMID: 32227412, PMCID: PMC7395670, DOI: 10.1002/anie.202001758.Peer-Reviewed Original ResearchMeSH KeywordsBinding SitesDimerizationGuanosine DiphosphateGuanosine TriphosphateHumansMagnetic Resonance SpectroscopyMolecular Dynamics SimulationProto-Oncogene Proteins p21(ras)ConceptsKRAS dimerizationParamagnetic relaxation enhancement NMR spectroscopyStructural basisΑ4-α5 interfaceEffector-binding siteParamagnetic relaxation enhancementActive GTPRaf activationSmall GTPasesDistinct structuresRaf kinaseGTPDimerizationRelaxation enhancementKRAS4bGTPasesR135E168ActivationKinaseProtomersHomodimerNanodiscsNMR spectroscopyMembrane
2018
Multiplexed Real-Time NMR GTPase Assay for Simultaneous Monitoring of Multiple Guanine Nucleotide Exchange Factor Activities from Human Cancer Cells and Organoids
Gebregiworgis T, Marshall C, Nishikawa T, Radulovich N, Sandí M, Fang Z, Rottapel R, Tsao M, Ikura M. Multiplexed Real-Time NMR GTPase Assay for Simultaneous Monitoring of Multiple Guanine Nucleotide Exchange Factor Activities from Human Cancer Cells and Organoids. Journal Of The American Chemical Society 2018, 140: 4473-4476. PMID: 29543440, DOI: 10.1021/jacs.7b13703.Peer-Reviewed Original ResearchMeSH KeywordsBiological AssayGTP PhosphohydrolasesGuanine Nucleotide Exchange FactorsHEK293 CellsHumansMagnetic Resonance SpectroscopyPancreatic NeoplasmsRhoA GTP-Binding ProteinConceptsGuanine nucleotide exchange factorsRhoA-specific Guanine Nucleotide Exchange FactorGuanine nucleotide exchange factor activityNucleotide exchange factor activityHigher GEF activityNucleotide exchange factorsImportant cellular functionsExchange factor activityExchange of GTPProtein of interestReal-time NMRRNA-seq dataCultured human cellsGEF assaysHuman cancer cellsSmall GTPasesExchange factorGEF activityCellular functionsIsotopic labeling schemesNucleotide exchangeGEF-H1GTPase assaysFunctional analysisHuman cells