1999
Identification and Characterization of Polycystin-2, thePKD2 Gene Product*
Cai Y, Maeda Y, Cedzich A, Torres V, Wu G, Hayashi T, Mochizuki T, Park J, Witzgall R, Somlo S. Identification and Characterization of Polycystin-2, thePKD2 Gene Product*. Journal Of Biological Chemistry 1999, 274: 28557-28565. PMID: 10497221, DOI: 10.1074/jbc.274.40.28557.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulum localizationPolycystin-2Gene productsPutative phosphorylation sitesProtein binding partnersProtein disulfide isomerasePKD2 gene productImmunofluorescent cell stainingIntegral membrane glycoproteinSubcellular fractionation studiesAutosomal dominant polycystic kidney diseaseEndo H resistanceGolgi proteinsPhosphorylation sitesSecond geneBinding partnerTruncation mutantsTranslation productsPlasma membraneHigh-mannose oligosaccharidesAcid regionEndoplasmic reticulumIntact cellsH resistanceMembrane glycoproteins
1997
Characterization of the Exon Structure of the Polycystic Kidney Disease 2 Gene (PKD2)
Hayashi T, Mochizuki T, Reynolds D, Wu G, Cai Y, Somlo S. Characterization of the Exon Structure of the Polycystic Kidney Disease 2 Gene (PKD2). Genomics 1997, 44: 131-136. PMID: 9286709, DOI: 10.1006/geno.1997.4851.Peer-Reviewed Original ResearchConceptsAG/GT rulePolycystic kidney disease 2 (PKD2) geneExon-intron structureIntegral membrane proteinsAutosomal dominant polycystic kidney diseaseTranslation start siteExon structurePositional cloningMembrane proteinsStart siteGenomic DNASplice acceptorPKD2 geneGenesExon 1Oligonucleotide primersHeteroduplex analysisPKD2Dominant polycystic kidney diseasePolycystic kidney diseaseMutation analysisCalcium channelsCloningSecond formExons