2010
Structural and Functional Modifications of Corneal Crystallin ALDH3A1 by UVB Light
Estey T, Chen Y, Carpenter JF, Vasiliou V. Structural and Functional Modifications of Corneal Crystallin ALDH3A1 by UVB Light. PLOS ONE 2010, 5: e15218. PMID: 21203538, PMCID: PMC3006428, DOI: 10.1371/journal.pone.0015218.Peer-Reviewed Original ResearchConceptsActive site CysNon-native aggregationNon-covalent interactionsAldehyde dehydrogenase 3A1MALDI-TOF mass spectrometryMammalian corneal epitheliumCorneal crystallinsSpectroscopic studiesChemical modificationUV-induced damageCys residuesGlucose-6-phosphate dehydrogenaseTertiary structureMass spectrometryMultifaceted roleResult of aggregationALDH3A1Enzymatic activityCorneal proteinsUV-induced inactivationOxidative stressProteinFunctional modificationsResiduesDirect absorptionAldehyde Dehydrogenase 1B1: Molecular Cloning and Characterization of a Novel Mitochondrial Acetaldehyde-Metabolizing Enzyme
Stagos D, Chen Y, Brocker C, Donald E, Jackson BC, Orlicky DJ, Thompson DC, Vasiliou V. Aldehyde Dehydrogenase 1B1: Molecular Cloning and Characterization of a Novel Mitochondrial Acetaldehyde-Metabolizing Enzyme. Drug Metabolism And Disposition 2010, 38: 1679-1687. PMID: 20616185, PMCID: PMC2957164, DOI: 10.1124/dmd.110.034678.Peer-Reviewed Original ResearchMeSH KeywordsAcetaldehydeAldehyde DehydrogenaseAldehyde Dehydrogenase 1 FamilyAldehyde Dehydrogenase, MitochondrialAmino Acid SequenceAnimalsBaculoviridaeBlotting, WesternCell LineCloning, MolecularEthanolGenetic VectorsHumansImmunohistochemistryInsectaMaleMiceMice, Inbred C57BLMice, KnockoutMitochondriaMolecular Sequence DataNADOrgan SpecificityOxidation-ReductionPlasmidsRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
2005
Glutamate Cysteine Ligase Catalysis DEPENDENCE ON ATP AND MODIFIER SUBUNIT FOR REGULATION OF TISSUE GLUTATHIONE LEVELS*
Chen Y, Shertzer HG, Schneider SN, Nebert DW, Dalton TP. Glutamate Cysteine Ligase Catalysis DEPENDENCE ON ATP AND MODIFIER SUBUNIT FOR REGULATION OF TISSUE GLUTATHIONE LEVELS*. Journal Of Biological Chemistry 2005, 280: 33766-33774. PMID: 16081425, DOI: 10.1074/jbc.m504604200.Peer-Reviewed Original Research