2021
MMAB promotes negative feedback control of cholesterol homeostasis
Goedeke L, Canfrán-Duque A, Rotllan N, Chaube B, Thompson BM, Lee RG, Cline GW, McDonald JG, Shulman GI, Lasunción MA, Suárez Y, Fernández-Hernando C. MMAB promotes negative feedback control of cholesterol homeostasis. Nature Communications 2021, 12: 6448. PMID: 34750386, PMCID: PMC8575900, DOI: 10.1038/s41467-021-26787-7.Peer-Reviewed Original ResearchMeSH KeywordsAlkyl and Aryl TransferasesAnimalsCell Line, TumorCholesterolCholesterol, LDLFeedback, PhysiologicalGene Expression ProfilingHeLa CellsHep G2 CellsHomeostasisHumansHydroxymethylglutaryl CoA ReductasesLiverMice, Inbred C57BLMice, KnockoutPromoter Regions, GeneticReceptors, LDLRNA InterferenceSterol Regulatory Element Binding Protein 2ConceptsCholesterol biosynthesisCholesterol homeostasisMouse hepatic cell lineIntegrative genomic strategyIntricate regulatory networkMaster transcriptional regulatorCellular cholesterol levelsHMGCR activityLDL-cholesterol uptakeCholesterol levelsHuman hepatic cellsSterol contentGenomic strategiesTranscriptional regulatorsRegulatory networksIntracellular cholesterol levelsGene expressionUnexpected roleHepatic cell linesBiosynthesisMMABIntracellular levelsCell linesHomeostasisExpression of SREBP2
2015
The miR-199–dynamin regulatory axis controls receptor-mediated endocytosis
Aranda JF, Canfrán-Duque A, Goedeke L, Suárez Y, Fernández-Hernando C. The miR-199–dynamin regulatory axis controls receptor-mediated endocytosis. Journal Of Cell Science 2015, 128: 3197-3209. PMID: 26163491, PMCID: PMC4582188, DOI: 10.1242/jcs.165233.Peer-Reviewed Original ResearchConceptsClathrin heavy chainReceptor-mediated endocytosisIntracellular traffickingLow-density lipoprotein receptorGene expressionMiR-199aSmall non-coding RNAsNon-coding RNAsTarget gene expressionDynamin genesEukaryotic cellsHuman cell linesEndocytic transportGTPase familyCav-1 expressionUnexpected layerCaveolin-1Intronic sequencesIntracellular transportPhysiological processesEndocytosisImportant regulatorMiR-199bCell linesGenes
2011
miR-33a/b contribute to the regulation of fatty acid metabolism and insulin signaling
Dávalos A, Goedeke L, Smibert P, Ramírez CM, Warrier NP, Andreo U, Cirera-Salinas D, Rayner K, Suresh U, Pastor-Pareja JC, Esplugues E, Fisher EA, Penalva LO, Moore KJ, Suárez Y, Lai EC, Fernández-Hernando C. miR-33a/b contribute to the regulation of fatty acid metabolism and insulin signaling. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 9232-9237. PMID: 21576456, PMCID: PMC3107310, DOI: 10.1073/pnas.1102281108.Peer-Reviewed Original ResearchConceptsFatty acid metabolismFatty acid oxidationMiR-33aInsulin receptor substrate 2Sirtuin 6Acid metabolismInsulin-signaling pathwayIntronic microRNAsSterol regulatory element-binding protein 2Acid oxidationHost genesKey enzymeHepatic cell linesMetabolic syndromeCarnitine palmitoyltransferase 1AMetabolic pathwaysSubstrate 2Cellular imbalanceProtein 2Cholesterol homeostasisGenesCell linesLevels of HDLPathwayMetabolism results
2002
Inhibition of cholesterol biosynthesis by Δ22-unsaturated phytosterols via competitive inhibition of sterol Δ24-reductase in mammalian cells
FERNÁNDEZ C, SUÁREZ Y, FERRUELO AJ, GÓMEZ-CORONADO D, LASUNCIÓN MA. Inhibition of cholesterol biosynthesis by Δ22-unsaturated phytosterols via competitive inhibition of sterol Δ24-reductase in mammalian cells. Biochemical Journal 2002, 366: 109-119. PMID: 12162789, PMCID: PMC1222779, DOI: 10.1042/bj20011777.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding, CompetitiveCaco-2 CellsCholesterolChromatography, High Pressure LiquidDose-Response Relationship, DrugHL-60 CellsHumansHypolipidemic AgentsKineticsMacrophagesMaleMiceMicrosomes, LiverModels, ChemicalOxidoreductasesPhytosterolsRatsRats, Sprague-DawleyStigmasterolTime FactorsTumor Cells, CulturedConceptsCholesterol biosynthesisCholesterol-lowering actionCholesterol-lowering agentsEffective hypocholesterolaemic agentHL-60 human cell linesRat liver microsomesIntestinal absorptionDietary phytosterolsLiver microsomesHypocholesterolaemic agentsLow intracellular concentrationsCaco-2Inhibition of sterolIncorporation of radioactivityHuman cell linesCell linesInhibitionSaturated side chainIntracellular concentrationCholesterolRelevant concentrationsRadioactivity incorporationPresent studyStrong inhibitionCompetitive inhibition
2001
Dose-dependent effects of lovastatin on cell cycle progression. Distinct requirement of cholesterol and non-sterol mevalonate derivatives
Martı́nez-Botas J, Ferruelo A, Suárez Y, Fernández C, Gómez-Coronado D, Lasunción M. Dose-dependent effects of lovastatin on cell cycle progression. Distinct requirement of cholesterol and non-sterol mevalonate derivatives. Biochimica Et Biophysica Acta 2001, 1532: 185-194. PMID: 11470239, DOI: 10.1016/s1388-1981(01)00125-1.Peer-Reviewed Original ResearchConceptsCell proliferationLow-density lipoprotein cholesterolCell cycle progressionDose-dependent effectCell cycle distributionCell cycleCycle progressionLipoprotein cholesterolConcentrations of lovastatinCholesterol supplyCycle distributionCholesterolLovastatinHuman cell linesCell linesCholesterol biosynthesisCholesterol-free mediumNormal cell cyclingM phaseProgressionProliferationPresent studyHL-60Mevalonate derivativesCell cycling