An Engineered Rare Codon Device for Optimization of Metabolic Pathways
Wang Y, Li C, Khan M, Wang Y, Ruan Y, Zhao B, Zhang B, Ma X, Zhang K, Zhao X, Ye G, Guo X, Feng G, He L, Ma G. An Engineered Rare Codon Device for Optimization of Metabolic Pathways. Scientific Reports 2016, 6: 20608. PMID: 26852704, PMCID: PMC4745014, DOI: 10.1038/srep20608.Peer-Reviewed Original ResearchConceptsRare codonsCognate tRNAMetabolic pathwaysExpression levelsProtein expressionTarget protein expressionAspartyl-tRNA synthetaseCodons AGGReporter geneRelevant enzymesCopy numberEscherichia coliCodonFatty acid yieldGenesSteady-state kineticsE. coliTRNAPathwayExpressionColiIntermediate levelsTwo-fold increaseSynthetaseAcid yield