2022
Multiplexed LNP-mRNA vaccination against pathogenic coronavirus species
Peng L, Fang Z, Renauer PA, McNamara A, Park JJ, Lin Q, Zhou X, Dong MB, Zhu B, Zhao H, Wilen CB, Chen S. Multiplexed LNP-mRNA vaccination against pathogenic coronavirus species. Cell Reports 2022, 40: 111160. PMID: 35921835, PMCID: PMC9294034, DOI: 10.1016/j.celrep.2022.111160.Peer-Reviewed Original ResearchConceptsAntibody responseCoronavirus speciesSequential vaccinationSARS-CoVAntigen-specific antibody responsesSARS-CoV-2 DeltaAdaptive immune cellsEffective immune responsePotent antibody responsesCOVID-19 vaccineSARS-CoV-2MRNA vaccine candidatesActivated B cellsSingle-cell RNA sequencing profilesRNA sequencing profilesSimultaneous vaccinationAntibody immunityVaccination scheduleImmune profileImmune cellsImmune responseVaccine candidatesMERS-CoV.Animal modelsB cellsVariant-specific vaccination induces systems immune responses and potent in vivo protection against SARS-CoV-2
Peng L, Renauer PA, Ökten A, Fang Z, Park JJ, Zhou X, Lin Q, Dong MB, Filler R, Xiong Q, Clark P, Lin C, Wilen CB, Chen S. Variant-specific vaccination induces systems immune responses and potent in vivo protection against SARS-CoV-2. Cell Reports Medicine 2022, 3: 100634. PMID: 35561673, PMCID: PMC9040489, DOI: 10.1016/j.xcrm.2022.100634.Peer-Reviewed Original ResearchConceptsImmune responseImmune cell populationsSARS-CoV-2 spikeAssessment of efficacySARS-CoV-2LNP-mRNABreakthrough infectionsCD8 TImmune profilingMRNA vaccinesPotent protectionT lymphocytesNeutralization activityDelta variantAnimal modelsPotent antibodiesRepertoire diversityCell responsesAuthentic virusSystemic increaseVariant lineagesClonal expansionCell populationsCOVID-19Vaccination
2019
Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells
Dong MB, Wang G, Chow RD, Ye L, Zhu L, Dai X, Park JJ, Kim HR, Errami Y, Guzman CD, Zhou X, Chen KY, Renauer PA, Du Y, Shen J, Lam SZ, Zhou JJ, Lannin DR, Herbst RS, Chen S. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell 2019, 178: 1189-1204.e23. PMID: 31442407, PMCID: PMC6719679, DOI: 10.1016/j.cell.2019.07.044.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBreast NeoplasmsCD8-Positive T-LymphocytesCell Line, TumorClustered Regularly Interspaced Short Palindromic RepeatsCytokinesFemaleHumansImmunologic MemoryImmunotherapyMaleMiceMice, KnockoutNF-kappa BProgrammed Cell Death 1 ReceptorRNA HelicasesRNA, Guide, CRISPR-Cas SystemsTranscriptomeConceptsCRISPR screensTarget discoveryGenome-scale CRISPR screensCD8 TRNA helicase DHX37Vivo CRISPR screensGenetic screenGenome scaleTranscriptomic profilingBiochemical interrogationAntigen-specific CD8 TAnti-tumor immune responseFunctional regulatorTriple-negative breast cancerDHX37Essential roleTim-3PD-1Cytokine productionTumor infiltrationImmunotherapy targetImmunotherapy settingsRegulatorBreast cancerT cellsPrecise spatio-temporal interruption of regulatory T cell-mediated CD8+ T cell suppression leads to tumor immunity
Zhou X, Zhao S, He Y, Geng S, Shi Y, Wang B. Precise spatio-temporal interruption of regulatory T cell-mediated CD8+ T cell suppression leads to tumor immunity. Cancer Research 2019, 79: canres.1250.2018. PMID: 30254146, DOI: 10.1158/0008-5472.can-18-1250.Peer-Reviewed Original ResearchConceptsTumor-draining lymph nodesRegulatory T cellsT cellsStrong tumor-suppressive effectSphingosine-1-phosphate receptor 1Low systemic side effectsT cell activityT cell suppressionSignificant tumor reductionSystemic side effectsAntitumor immune activationTumor-suppressive effectsDraining lymphMinimal blockadeTreg ablationTreg inhibitionLymph nodesTumor immunityTumor inoculationClinical evidenceImmune activationImmune suppressionTumor reductionCD8Cell suppression
2015
Herpes Simplex Virus 1 Suppresses the Function of Lung Dendritic Cells via Caveolin-1
Wu B, Geng S, Bi Y, Liu H, Hu Y, Li X, Zhang Y, Zhou X, Zheng G, He B, Wang B. Herpes Simplex Virus 1 Suppresses the Function of Lung Dendritic Cells via Caveolin-1. MSphere 2015, 22: 883-895. PMID: 26018534, PMCID: PMC4519715, DOI: 10.1128/cvi.00170-15.Peer-Reviewed Original ResearchConceptsInducible nitric oxide synthaseDendritic cellsHSV-1 infectionCav-1HSV-1Nitric oxideHerpes simplex virus 1 (HSV-1) infectionLung dendritic cellsSimplex virus 1 infectionLung pathological changesCav-1 knockout miceNitric oxide synthaseVirus-1 infectionWild-type miceHost antiviral responseHerpes simplex virus 1Cav-1 deficiencySimplex virus 1Adoptive transferCaveolin-1Cav-1 knockdownINOS inhibitorOxide synthasePathological changesTherapeutic approaches