2001
Membrane Properties Underlying Patterns of GABA-Dependent Action Potentials in Developing Mouse Hypothalamic Neurons
Wang Y, Gao X, van den Pol A. Membrane Properties Underlying Patterns of GABA-Dependent Action Potentials in Developing Mouse Hypothalamic Neurons. Journal Of Neurophysiology 2001, 86: 1252-1265. PMID: 11535674, DOI: 10.1152/jn.2001.86.3.1252.Peer-Reviewed Original ResearchConceptsHypothalamic neuronsAction potentialsMouse hypothalamusMultiple spikesSpike thresholdFrequency of GABAMediobasal hypothalamic neuronsProbability of GABAGlutamate receptor antagonistsConventional whole cellMouse hypothalamic neuronsMultiple action potentialsPostnatal day 2Intrinsic membrane propertiesMean reversal potentialIonotropic glutamate receptorsSimilar spike patternsPeaks of depolarizationGABA excitationMembrane potentialReceptor antagonistSpike patternsExcitatory activityGlutamate receptorsSynaptic transmissionLateral hypothalamus: Early developmental expression and response to hypocretin (orexin)
Van Den Pol A, Patrylo P, Ghosh P, Gao X. Lateral hypothalamus: Early developmental expression and response to hypocretin (orexin). The Journal Of Comparative Neurology 2001, 433: 349-363. PMID: 11298360, DOI: 10.1002/cne.1144.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAnimals, NewbornBrainCalciumCarrier ProteinsCells, CulturedElectrophysiologyEmbryo, MammalianHypothalamic Area, LateralImmunohistochemistryIn Vitro TechniquesIntracellular Signaling Peptides and ProteinsNeuronsNeuropeptidesOrexin ReceptorsOrexinsRatsRats, Sprague-DawleyReceptors, G-Protein-CoupledReceptors, NeuropeptideRNA, MessengerConceptsLateral hypothalamic areaSynaptic activityLH neuronsNeuronal activitySleep regulationWhole-cell patch-clamp recordingsRobust increaseAdult central nervous systemEndocrine controlPostnatal day 1Day of birthCentral nervous systemPatch-clamp recordingsVoltage-clamp recordingsEmbryonic day 19Hypocretin-1Excitatory influenceHypothalamic areaHypocretin-2Spinal cordMature brainFood intakeHypocretin systemLH cellsReceptor mRNA
1999
Neurotrophin‐3 potentiates excitatory GABAergic synaptic transmission in cultured developing hypothalamic neurones of the rat
Gao X, van den Pol A. Neurotrophin‐3 potentiates excitatory GABAergic synaptic transmission in cultured developing hypothalamic neurones of the rat. The Journal Of Physiology 1999, 518: 81-95. PMID: 10373691, PMCID: PMC2269394, DOI: 10.1111/j.1469-7793.1999.0081r.x.Peer-Reviewed Original ResearchMeSH Keywords6-Cyano-7-nitroquinoxaline-2,3-dioneAnimalsCalciumCarbazolesCells, CulturedElectric StimulationEnzyme InhibitorsExcitatory Amino Acid AntagonistsExcitatory Postsynaptic PotentialsGamma-Aminobutyric AcidHypothalamusIndole AlkaloidsMembrane PotentialsNeuronsNeurotrophin 3Patch-Clamp TechniquesProtein-Tyrosine KinasesRatsSynapsesSynaptic TransmissionTetrodotoxinConceptsSpontaneous postsynaptic currentsFrequency of GABANT-3 effectsNeurotrophin-3Postsynaptic currentsHypothalamic neuronesMature neuronesWhole-cell patch-clamp recordingsMiniature post-synaptic currentsGABAergic synaptic transmissionMiniature postsynaptic currentsDose-dependent potentiationPresence of tetrodotoxinAction potential frequencyPeripheral nervous systemPatch-clamp recordingsTyrosine kinase inhibitorsMean amplitudePost-synaptic currentsNon-selective tyrosine kinase inhibitorRat hypothalamic neuronesGABA excitationGABA releaseGABAergic synapsesPresynaptic locus
1998
Glutamate Inhibits GABA Excitatory Activity in Developing Neurons
van den Pol A, Gao X, Patrylo P, Ghosh P, Obrietan K. Glutamate Inhibits GABA Excitatory Activity in Developing Neurons. Journal Of Neuroscience 1998, 18: 10749-10761. PMID: 9852609, PMCID: PMC6793361, DOI: 10.1523/jneurosci.18-24-10749.1998.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornCalciumCells, CulturedCerebral CortexEmbryo, MammalianExcitatory Postsynaptic PotentialsGABA AntagonistsGamma-Aminobutyric AcidGene ExpressionGlutamic AcidGramicidinHypothalamusIn Vitro TechniquesNeuronsPatch-Clamp TechniquesPresynaptic TerminalsPropionatesRatsRats, Sprague-DawleyReceptors, Metabotropic GlutamateSpinal CordConceptsGroup III mGluRsExcitatory actionGABA activityGroup II metabotropic glutamate receptorsFura-2 digital imagingSynapse formationBrain developmentInhibitory roleAgonist L-AP4Excitatory GABA actionsGlutamate-mediated inhibitionMetabotropic glutamate receptorsSpinal cord neuronsMajor inhibitory neurotransmitterWhole-cell recordingsSimilar inhibitory actionBrain GABAEarly brain developmentExcitatory roleGABA releaseL-AP4Excitatory transmitterGABA actionHypothalamic neuronsCord neuronsPresynaptic and Postsynaptic Actions and Modulation of Neuroendocrine Neurons by a New Hypothalamic Peptide, Hypocretin/Orexin
van den Pol A, Gao X, Obrietan K, Kilduff T, Belousov A. Presynaptic and Postsynaptic Actions and Modulation of Neuroendocrine Neurons by a New Hypothalamic Peptide, Hypocretin/Orexin. Journal Of Neuroscience 1998, 18: 7962-7971. PMID: 9742163, PMCID: PMC6793026, DOI: 10.1523/jneurosci.18-19-07962.1998.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsArcuate Nucleus of HypothalamusCadmiumCalciumCarrier ProteinsCells, CulturedDose-Response Relationship, DrugElectric StimulationEnzyme InhibitorsEvoked PotentialsFeeding BehaviorFluorescent DyesFura-2Gamma-Aminobutyric AcidGlutamic AcidIntracellular Signaling Peptides and ProteinsMembrane PotentialsMolecular Sequence DataNeuronsNeuropeptidesNeurosecretory SystemsNeurotransmitter AgentsOrexinsPatch-Clamp TechniquesPresynaptic TerminalsSignal TransductionTetrodotoxinThapsigarginConceptsLateral hypothalamusSynaptic activityNeuroendocrine neuronsWhole-cell patch-clamp recordingsNew hypothalamic peptidesLateral hypothalamic neuronsAmino acid transmittersHypothalamic regulatory systemPatch-clamp recordingsHypocretin/orexinPerifornical areaPostsynaptic actionsOrexigenic peptideHypocretin-1Hypothalamic circuitsHypocretin peptidesHypothalamic neuronsHypothalamic slicesArcuate nucleusHypocretin-2Postsynaptic receptorsAxonal inputsHypothalamic peptidesFeeding centerAxon terminals