2001
Distinction between signaling mechanisms in lipid rafts vs. caveolae
Sowa G, Pypaert M, Sessa W. Distinction between signaling mechanisms in lipid rafts vs. caveolae. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 14072-14077. PMID: 11707586, PMCID: PMC61169, DOI: 10.1073/pnas.241409998.Peer-Reviewed Original ResearchConceptsCav-1Raft domainsLipid raftsCholesterol-rich lipid raft domainsLipid raft domainsCaveolae assemblyEndothelial nitric oxide synthaseCaveolae biogenesisAcylated proteinsSignal transductionSpatial regulationPlasma membraneNegative regulationCaveolin-1CaveolaeFirst clear exampleRaftsPhysical interactionProteinCellsRegulationENOS functionBiogenesisDomainClear example
2000
Direct Interaction between Endothelial Nitric-oxide Synthase and Dynamin-2 IMPLICATIONS FOR NITRIC-OXIDE SYNTHASE FUNCTION*
Cao S, Yao J, McCabe T, Yao Q, Katusic Z, Sessa W, Shah V. Direct Interaction between Endothelial Nitric-oxide Synthase and Dynamin-2 IMPLICATIONS FOR NITRIC-OXIDE SYNTHASE FUNCTION*. Journal Of Biological Chemistry 2000, 276: 14249-14256. PMID: 11120737, DOI: 10.1074/jbc.m006258200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaBlotting, WesternCalcimycinCattleCell LineDose-Response Relationship, DrugDynamin IDynaminsEndothelium, VascularGlutathione TransferaseGolgi ApparatusGTP PhosphohydrolasesIonophoresKineticsMicroscopy, ConfocalMicroscopy, FluorescenceNitric Oxide SynthaseNitric Oxide Synthase Type IIIPrecipitin TestsProtein BindingProtein BiosynthesisRatsRecombinant Fusion ProteinsTransfectionConceptsDynamin 2Bovine aortic endothelial cellsRecombinant eNOS proteinDirect protein-protein interactionDouble-label confocal immunofluorescenceProtein-protein interactionsSpecific protein interactionsDirect interactionClone 9 cellsEndothelial nitric oxide synthaseMembrane compartmentsLarge GTPaseGlutathione S-transferaseProtein interactionsNovel functionDynaminECV-304 cellsSynthase functionIntracellular signalsMembrane distributionConfocal immunofluorescenceFluorescent proteinENOS regulationGreen fluorescentProteinEnhanced Electron Flux and Reduced Calmodulin Dissociation May Explain “Calcium-independent” eNOS Activation by Phosphorylation*
McCabe T, Fulton D, Roman L, Sessa W. Enhanced Electron Flux and Reduced Calmodulin Dissociation May Explain “Calcium-independent” eNOS Activation by Phosphorylation*. Journal Of Biological Chemistry 2000, 275: 6123-6128. PMID: 10692402, DOI: 10.1074/jbc.275.9.6123.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalmodulinCattleDimerizationEgtazic AcidElectronsEnzyme ActivationKineticsMutationNADH DehydrogenaseNADPNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIIPhosphorylationProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktStatic ElectricityConceptsSerine 1179Reductase domainCalmodulin dissociationProtein kinase AktWild-type eNOSBovine endothelial nitric oxide synthaseEndothelial nitric oxide synthaseKinase AktRate-limiting stepReductase activityPhosphorylationENOS activationNOS functionPotential mechanismsAspartateENOS catalytic activityENOS activityCytochrome c reductionAktCalmodulinDomainProteinMutationsProductionActivity
1996
Endothelial Nitric Oxide Synthase Is Regulated by Tyrosine Phosphorylation and Interacts with Caveolin-1*
García-Cardeña G, Fan R, Stern D, Liu J, Sessa W. Endothelial Nitric Oxide Synthase Is Regulated by Tyrosine Phosphorylation and Interacts with Caveolin-1*. Journal Of Biological Chemistry 1996, 271: 27237-27240. PMID: 8910295, DOI: 10.1074/jbc.271.44.27237.Peer-Reviewed Original ResearchConceptsNovel regulatory mechanismTyrosine phosphorylationCaveolin-1Bovine aortic endothelial cellsRegulatory mechanismsProtein tyrosine phosphatase inhibitorCaveolin-interacting proteinsPhosphoamino acid analysisTyrosine phosphatase inhibitorTreatment of BAECBovine lung microvascular endothelial cellsEndothelial nitric oxide synthaseSubcellular traffickingPhosphatase inhibitorCoat proteinEndothelial cellsMetabolic labelingSodium orthovanadatePhosphorylationCaveolaeAortic endothelial cellsLung microvascular endothelial cellsProteinAcid analysisImmunoprecipitationCharacterization of Bovine Endothelial Nitric Oxide Synthase Expressed inE. coli
Martasek P, Liu Q, Liu J, Roman L, Gross S, Sessa W, Masters B. Characterization of Bovine Endothelial Nitric Oxide Synthase Expressed inE. coli. Biochemical And Biophysical Research Communications 1996, 219: 359-365. PMID: 8604992, DOI: 10.1006/bbrc.1996.0238.Peer-Reviewed Original ResearchConceptsE. coliBaculovirus expression systemBovine endothelial nitric oxide synthaseSDS/PAGEHEK-293 cellsChaperonin GroELHeterologous expressionRecombinant enzymeExpression systemExpression vectorActive enzymeFormation assaysEnzymatic activityColiEnzymeTissue sourcesEndothelial constitutive nitric oxide synthaseProteinSingle bandSynthaseNitric oxide synthaseAbsorbance maximumEndothelial nitric oxide synthase
1995
The Golgi Association of Endothelial Nitric Oxide Synthase Is Necessary for the Efficient Synthesis of Nitric Oxide(∗)
Sessa W, Garca-Cardea G, Liu J, Keh A, Pollock J, Bradley J, Thiru S, Braverman I, Desai K. The Golgi Association of Endothelial Nitric Oxide Synthase Is Necessary for the Efficient Synthesis of Nitric Oxide(∗). Journal Of Biological Chemistry 1995, 270: 17641-17644. PMID: 7543089, DOI: 10.1074/jbc.270.30.17641.Peer-Reviewed Original ResearchConceptsHEK-293 cellsHeterologous expression systemEndothelial nitric oxide synthaseNovel GolgiGolgi associationExpression systemIntracellular signalsCultured endothelial cellsGolgiIntact blood vesselsEnzymeParticulate enzymeProteinEndothelial cellsCellsSynthaseNitric oxide synthaseOxide synthaseBlood vesselsCompartmentalizationNitric oxideTarget
1994
The Nitric Oxide Synthase Family of Proteins
Sessa W. The Nitric Oxide Synthase Family of Proteins. Journal Of Vascular Research 1994, 31: 131-143. PMID: 7511942, DOI: 10.1159/000159039.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid OxidoreductasesAmino Acid SequenceAnimalsArginineCardiovascular SystemCloning, MolecularConsensus SequenceDNAGene Expression Regulation, EnzymologicHumansImmune SystemMolecular Sequence DataNervous SystemNitric OxideNitric Oxide SynthasePhosphorylationSequence AlignmentSequence Homology, Amino AcidConceptsCytochrome P450 reductase domainNitric oxide synthaseP450 reductase domainNitric oxide synthase (NOS) familySynthase familyMammalian enzymeSeparate genesReductase domainMolecular biologyNovel familyIsoforms of NOSImportant familyNitric oxideProteinEndothelial nitric oxide synthaseEnzyme nitric oxide synthaseGuanidino nitrogenImportant bio-regulatory moleculeOxide synthaseFamilyL-arginineCardiovascular systemRecent advancesGenesEnzymology