2019
Cardiomyocyte orientation modulated by the Numb family proteins–N-cadherin axis is essential for ventricular wall morphogenesis
Miao L, Li J, Li J, Lu Y, Shieh D, Mazurkiewicz JE, Barroso M, Schwarz JJ, Xin HB, Singer HA, Vincent PA, Zhong W, Radice GL, Wan LQ, Fan ZC, Huang G, Wu M. Cardiomyocyte orientation modulated by the Numb family proteins–N-cadherin axis is essential for ventricular wall morphogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 15560-15569. PMID: 31300538, PMCID: PMC6681736, DOI: 10.1073/pnas.1904684116.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCadherinsHeart VentriclesIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceMice, KnockoutMyocytes, CardiacNerve Tissue ProteinsOrganogenesisConceptsWall morphogenesisMembrane localizationPlasma membraneCellular orientationSingle-cell lineage tracingMitotic spindle orientationDouble knockout heartsN-cadherin overexpressionPostendocytic recyclingSpindle orientationRecycling endosomesN-cadherin levelsEndocytic organellesKnockin lineLineage tracingMorphogenesisCellular behaviorTrabecular morphogenesisNull cardiomyocytesNumbVentricular compactionMosaic modelControl cellsLocalizationMdKO
2014
Precardiac deletion of Numb and Numblike reveals renewal of cardiac progenitors
Shenje LT, Andersen P, Uosaki H, Fernandez L, Rainer PP, Cho GS, Lee DI, Zhong W, Harvey RP, Kass DA, Kwon C. Precardiac deletion of Numb and Numblike reveals renewal of cardiac progenitors. ELife 2014, 3: e02164. PMID: 24843018, PMCID: PMC4007206, DOI: 10.7554/elife.02164.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineageGene DeletionHeartIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceMice, KnockoutMosaicismMyocytes, CardiacNerve Tissue ProteinsStem CellsConceptsCardiac progenitor cells
2013
Numb family proteins are essential for cardiac morphogenesis and progenitor differentiation
Zhao C, Guo H, Li J, Myint T, Pittman W, Yang L, Zhong W, Schwartz RJ, Schwarz JJ, Singer HA, Tallquist MD, Wu M. Numb family proteins are essential for cardiac morphogenesis and progenitor differentiation. Development 2013, 141: 281-295. PMID: 24335256, PMCID: PMC3879810, DOI: 10.1242/dev.093690.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell LineageCell ProliferationEmbryonic Stem CellsFemaleHeartHeart Defects, CongenitalIntracellular Signaling Peptides and ProteinsMaleMembrane ProteinsMiceMice, KnockoutMice, TransgenicMorphogenesisMyocardiumMyocytes, CardiacNerve Tissue ProteinsPregnancyReceptor, Notch1Signal TransductionConceptsNumb family proteinsCardiac progenitor differentiationCardiac progenitor cell differentiationSecond heart fieldProgenitor differentiationCardiac morphogenesisEndocytosis-dependent mannerProgenitor cell differentiationFamily proteinsCell differentiationOFT alignmentMultiple progenitor cell typesCell fate determinantsLate cardiac developmentEarly embryonic lethalityMRNA deep sequencingProgenitor cell typesSuppression of Notch1Fate determinantsNotch1 intracellular domainEmbryonic lethalEmbryonic lethalityReporter linesDifferentiation defectsHeart field
2005
The Notch Regulator Numb Links the Notch and TCR Signaling Pathways
Anderson AC, Kitchens EA, Chan SW, St. Hill C, Jan YN, Zhong W, Robey EA. The Notch Regulator Numb Links the Notch and TCR Signaling Pathways. The Journal Of Immunology 2005, 174: 890-897. PMID: 15634911, DOI: 10.4049/jimmunol.174.2.890.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen-Presenting CellsCell DifferentiationCells, CulturedGene DeletionIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceMice, TransgenicNerve Tissue ProteinsReceptor, Notch1Receptors, Antigen, T-CellReceptors, Cell SurfaceSignal TransductionT-LymphocytesThymus GlandTranscription FactorsConceptsT cell developmentTCR signaling pathwaysCell developmentSignaling pathwaysAbsence of NumbT cell-APC interactionsInhibitor of NotchProtein NumbImportant adapterRelated proteinsNumbAPC contactMature T cellsTCR signalsCell membraneT cell activationPhysical interactionPathwayCell activationNotchImportant roleNumblikeProteinMutationsCoclusters
2004
Targeted deletion of numb and numblike in sensory neurons reveals their essential functions in axon arborization
Huang EJ, Li H, Tang AA, Wiggins AK, Neve RL, Zhong W, Jan LY, Jan YN. Targeted deletion of numb and numblike in sensory neurons reveals their essential functions in axon arborization. Genes & Development 2004, 19: 138-151. PMID: 15598981, PMCID: PMC540232, DOI: 10.1101/gad.1246005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsIntegrasesIntracellular Signaling Peptides and ProteinsLysosomesMembrane ProteinsMiceMice, TransgenicMutagenesis, Site-DirectedNerve Tissue ProteinsNeurons, AfferentReceptor, Notch1Receptors, Cell SurfaceSequence DeletionTranscription FactorsTransport VesiclesConceptsNuclear Notch1Sensory neuronsAxon arborizationAccumulation of Notch1Conditional mouse mutantsAxon branch pointsAfferent fibersSensory gangliaAxonal arborizationAxonal lengthSevere reductionAxon lengthArborizationCre recombinaseNotch1Endocytic-lysosomal pathwayTargeted deletionSignificant increaseUnknown mechanismNeuronsOverexpression of NumbMouse mutantsGangliaNeurogenesisPathwayContinuing role for mouse Numb and Numbl in maintaining progenitor cells during cortical neurogenesis
Petersen PH, Zou K, Krauss S, Zhong W. Continuing role for mouse Numb and Numbl in maintaining progenitor cells during cortical neurogenesis. Nature Neuroscience 2004, 7: 803-811. PMID: 15273690, DOI: 10.1038/nn1289.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsEmbryo, MammalianGene TargetingImmunohistochemistryIn Situ HybridizationIn Situ Nick-End LabelingIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceMice, KnockoutMice, Mutant StrainsNeocortexNerve Tissue ProteinsNeuronsStem CellsConceptsMouse NumbProgenitor cellsEarly neurogenesisAsymmetric cell divisionProgenitor cell maintenanceLoss of NumbNeural progenitor cellsProgenitor cell depletionDrosophila melanogasterCell fateCell divisionNeuronal fateNumblNumbCortical neurogenesisStem cellsGeneral mechanismDorsal forebrainNeurogenesisCellsNervous systemFateSpecific malformationsDifferent neuronsMelanogaster
2003
Diversifying Neural Cells through Order of Birth and Asymmetry of Division
Zhong W. Diversifying Neural Cells through Order of Birth and Asymmetry of Division. Neuron 2003, 37: 11-14. PMID: 12526768, DOI: 10.1016/s0896-6273(02)01178-9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell DivisionCell LineageDrosophila melanogasterDrosophila ProteinsHumansIntracellular Signaling Peptides and ProteinsJuvenile HormonesNerve Tissue ProteinsNervous SystemNeuronsStem CellsConceptsAsymmetry of divisionCell fate diversificationCell fate determinationAsymmetric cell divisionMature nervous systemFate diversificationDrosophila genesFate determinationCell divisionCell typesProgenitor cellsNeural cellsDevelopmental neurobiologyNervous systemCommon setCellsDivisionGenesDiversityDiversificationKey questions
2002
Progenitor cell maintenance requires numb and numblike during mouse neurogenesis
Petersen PH, Zou K, Hwang JK, Jan YN, Zhong W. Progenitor cell maintenance requires numb and numblike during mouse neurogenesis. Nature 2002, 419: 929-934. PMID: 12410312, DOI: 10.1038/nature01124.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAnimalsCell DeathCell DifferentiationCell DivisionCell LineageDrosophila ProteinsGene DeletionIntracellular Signaling Peptides and ProteinsJuvenile HormonesMiceMice, KnockoutMotor NeuronsNerve Tissue ProteinsNervous SystemStem CellsTime FactorsConceptsMouse NumbNeural progenitor cellsMolecular mechanismsProgenitor cellsProgenitor cell maintenanceMammalian nervous systemOnset of neurogenesisConserved homologuesMutant embryosCourse of neurogenesisCell maintenanceMouse neurogenesisNeuronal fateNumbDividing cellsNeural cellsEarly neuronsNervous systemCritical roleProgenitorsCellsNeurogenesisDrosophilaTemporal patternsEmbryogenesis
1997
Differential expression of mammalian Numb, Numblike and Notch1 suggests distinct roles during mouse cortical neurogenesis
Zhong W, Jiang M, Weinmaster G, Jan L, Jan Y. Differential expression of mammalian Numb, Numblike and Notch1 suggests distinct roles during mouse cortical neurogenesis. Development 1997, 124: 1887-1897. PMID: 9169836, DOI: 10.1242/dev.124.10.1887.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCerebral CortexCloning, MolecularCytoplasmDrosophilaDrosophila ProteinsGene Expression Regulation, DevelopmentalGenesIntracellular Signaling Peptides and ProteinsJuvenile HormonesMembrane ProteinsMiceModels, NeurologicalMolecular Sequence DataMorphogenesisMutationNerve Tissue ProteinsNervous SystemNeuronsOrgan SpecificityReceptor, Notch1Receptors, Cell SurfaceRecombinant Fusion ProteinsRNA, MessengerSequence Homology, Amino AcidStem CellsTranscription FactorsConceptsDaughter cellsNeural precursorsMammalian cortical neurogenesisAsymmetric cell divisionSignificant sequence similarityMouse cortical neurogenesisCortical neurogenesisEmbryonic nervous systemFunction mutant embryosCell-cell interactionsCell-intrinsic mechanismsMammalian NumbMouse NumbNumb lossProgenitor cell proliferationDrosophila neurogenesisMammalian genesMutant embryosCell-extrinsic mechanismsSequence similarityCell divisionDifferential segregationNumblikePostmitotic neuronsNumb