2005
The Notch Regulator Numb Links the Notch and TCR Signaling Pathways
Anderson AC, Kitchens EA, Chan SW, St. Hill C, Jan YN, Zhong W, Robey EA. The Notch Regulator Numb Links the Notch and TCR Signaling Pathways. The Journal Of Immunology 2005, 174: 890-897. PMID: 15634911, DOI: 10.4049/jimmunol.174.2.890.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen-Presenting CellsCell DifferentiationCells, CulturedGene DeletionIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceMice, TransgenicNerve Tissue ProteinsReceptor, Notch1Receptors, Antigen, T-CellReceptors, Cell SurfaceSignal TransductionT-LymphocytesThymus GlandTranscription FactorsConceptsT cell developmentTCR signaling pathwaysCell developmentSignaling pathwaysAbsence of NumbT cell-APC interactionsInhibitor of NotchProtein NumbImportant adapterRelated proteinsNumbAPC contactMature T cellsTCR signalsCell membraneT cell activationPhysical interactionPathwayCell activationNotchImportant roleNumblikeProteinMutationsCoclusters
2004
Targeted deletion of numb and numblike in sensory neurons reveals their essential functions in axon arborization
Huang EJ, Li H, Tang AA, Wiggins AK, Neve RL, Zhong W, Jan LY, Jan YN. Targeted deletion of numb and numblike in sensory neurons reveals their essential functions in axon arborization. Genes & Development 2004, 19: 138-151. PMID: 15598981, PMCID: PMC540232, DOI: 10.1101/gad.1246005.Peer-Reviewed Original ResearchConceptsNuclear Notch1Sensory neuronsAxon arborizationAccumulation of Notch1Conditional mouse mutantsAxon branch pointsAfferent fibersSensory gangliaAxonal arborizationAxonal lengthSevere reductionAxon lengthArborizationCre recombinaseNotch1Endocytic-lysosomal pathwayTargeted deletionSignificant increaseUnknown mechanismNeuronsOverexpression of NumbMouse mutantsGangliaNeurogenesisPathway
1999
DLX‐1, DLX‐2, and DLX‐5 expression define distinct stages of basal forebrain differentiation
Eisenstat D, Liu J, Mione M, Zhong W, Yu G, Anderson S, Ghattas I, Puelles L, Rubenstein J. DLX‐1, DLX‐2, and DLX‐5 expression define distinct stages of basal forebrain differentiation. The Journal Of Comparative Neurology 1999, 414: 217-237. PMID: 10516593, DOI: 10.1002/(sici)1096-9861(19991115)414:2<217::aid-cne6>3.0.co;2-i.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsAntibody SpecificityBasal GangliaCell DifferentiationCell NucleusCerebral VentriclesCytoplasmGene Expression Regulation, DevelopmentalHomeodomain ProteinsIn Situ HybridizationIsomerismMiceMitosisMutagenesisNeuronsProsencephalonRecombinant ProteinsRNA, MessengerS PhaseTranscription FactorsConceptsDlx-2Dlx-1Dlx-5Dlx genesDlx-5 expressionEmbryonic day 10.5Ventricular zoneDlx proteinsDlx familyM phase cellsCraniofacial morphogenesisHomeobox genesSingle mutantsDaughter cellsDay 10.5GenesForebrain differentiationProteinSame cellsMouse forebrainMild phenotypeCellsDifferentiationExpressionDistinct stages
1997
Differential expression of mammalian Numb, Numblike and Notch1 suggests distinct roles during mouse cortical neurogenesis
Zhong W, Jiang M, Weinmaster G, Jan L, Jan Y. Differential expression of mammalian Numb, Numblike and Notch1 suggests distinct roles during mouse cortical neurogenesis. Development 1997, 124: 1887-1897. PMID: 9169836, DOI: 10.1242/dev.124.10.1887.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCerebral CortexCloning, MolecularCytoplasmDrosophilaDrosophila ProteinsGene Expression Regulation, DevelopmentalGenesIntracellular Signaling Peptides and ProteinsJuvenile HormonesMembrane ProteinsMiceModels, NeurologicalMolecular Sequence DataMorphogenesisMutationNerve Tissue ProteinsNervous SystemNeuronsOrgan SpecificityReceptor, Notch1Receptors, Cell SurfaceRecombinant Fusion ProteinsRNA, MessengerSequence Homology, Amino AcidStem CellsTranscription FactorsConceptsDaughter cellsNeural precursorsMammalian cortical neurogenesisAsymmetric cell divisionSignificant sequence similarityMouse cortical neurogenesisCortical neurogenesisEmbryonic nervous systemFunction mutant embryosCell-cell interactionsCell-intrinsic mechanismsMammalian NumbMouse NumbNumb lossProgenitor cell proliferationDrosophila neurogenesisMammalian genesMutant embryosCell-extrinsic mechanismsSequence similarityCell divisionDifferential segregationNumblikePostmitotic neuronsNumb
1994
Tissue-specific regulation of mouse hepatocyte nuclear factor 4 expression.
Zhong W, Mirkovitch J, Darnell J. Tissue-specific regulation of mouse hepatocyte nuclear factor 4 expression. Molecular And Cellular Biology 1994, 14: 7276-7284. PMID: 7523862, PMCID: PMC359262, DOI: 10.1128/mcb.14.11.7276.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsBinding SitesDeoxyribonuclease IDNA ProbesDNA-Binding ProteinsGene Expression RegulationHepatocyte Nuclear Factor 4KidneyLiverLiver Neoplasms, ExperimentalMiceMice, TransgenicMolecular Sequence DataPhosphoproteinsPromoter Regions, GeneticRNATissue DistributionTranscription FactorsTransfectionTumor Cells, CulturedConceptsTransient transfection assaysLiver-enriched transcription factorsHNF-4HNF-4 geneLiver-specific expressionTranscription factorsPresence of DNase I-hypersensitive sitesHNF-1 binding siteDNase I hypersensitive sitesHepatocyte nuclear factor 4Distal enhancer elementsDNase hypersensitive sitesHepatoma-specific expressionSteroid hormone receptor superfamilyHNF-4 expressionTissue-specific expressionHNF-1 alphaHormone receptor superfamilyExpression of HNF-4RNA startDNA elementsEnhancer elementsHNF-1Promoter regionReporter constructs
1993
The expression pattern of a Drosophila homolog to the mouse transcription factor HNF‐4 suggests a determinative role in gut formation.
Zhong W, Sladek F, Darnell J. The expression pattern of a Drosophila homolog to the mouse transcription factor HNF‐4 suggests a determinative role in gut formation. The EMBO Journal 1993, 12: 537-544. PMID: 8440243, PMCID: PMC413236, DOI: 10.1002/j.1460-2075.1993.tb05685.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsChromosome DeletionChromosome MappingDNA-Binding ProteinsDrosophilaGene ExpressionHepatocyte Nuclear Factor 4In Situ HybridizationIntestinal MucosaIntestinesKidneyLiverMiceMolecular Sequence DataPhenotypePhosphoproteinsRNA, MessengerSequence Homology, Amino AcidTranscription FactorsConceptsDNA-binding domainDrosophila genesGut formationHNF-4Binding domainIdentical DNA binding domainsZinc finger DNA-binding domainHepatocyte nuclear factor 3Transcription factor HNF-4Amino acidsGroup of genesLiver-specific gene expressionDrosophila homologDrosophila embryoDrosophila mutantsMouse genesHNF-3Cross-hybridizationLate embryogenesisChromosomal deletionsDrosophilaFat bodyGene expressionExpression patternsGenes
1992
Murine chromosomal location of four hepatocyte-enriched transcription factors: HNf-3α, HNF-3β, HNF-3γ, and HNF-4
Avraham K, Prezioso V, Chen W, Lai E, Sladek F, Zhong W, Darnell J, Jenkins N, Copeland N. Murine chromosomal location of four hepatocyte-enriched transcription factors: HNf-3α, HNF-3β, HNF-3γ, and HNF-4. Genomics 1992, 13: 264-268. PMID: 1612587, DOI: 10.1016/0888-7543(92)90241-j.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsChromosome MappingCrosses, GeneticDigestive SystemDNA-Binding ProteinsGene Expression RegulationGenetic MarkersHepatocyte Nuclear Factor 3-alphaHepatocyte Nuclear Factor 3-betaHepatocyte Nuclear Factor 3-gammaHepatocyte Nuclear Factor 4HumansMiceMice, Inbred C57BLMuridaeNuclear ProteinsPhosphoproteinsSpecies SpecificityTranscription FactorsConceptsHNF-3 familyHepatocyte-enriched transcription factorsHNF-3Transcription factorsHNF-4Positive-acting transcription factorsHepatocyte nuclear factor 3Analysis of restriction fragment length polymorphismsInterspecific backcross miceDNA-binding domainHNF-3 alphaRestriction fragment length polymorphismFragment length polymorphismDrosophila genesChromosomal locationMouse chromosomeRegulatory regionsHNF-3BBinding domainBackcross miceLength polymorphismEndoderm developmentGut cellsGenesBinding sites
1990
Liver-enriched transcription factor HNF-4 is a novel member of the steroid hormone receptor superfamily.
Sladek F, Zhong W, Lai E, Darnell J. Liver-enriched transcription factor HNF-4 is a novel member of the steroid hormone receptor superfamily. Genes & Development 1990, 4: 2353-2365. PMID: 2279702, DOI: 10.1101/gad.4.12b.2353.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCell NucleusCloning, MolecularDNA-Binding ProteinsGene ExpressionHepatocyte Nuclear Factor 4LiverMacromolecular SubstancesMaleMolecular Sequence DataMultigene FamilyOligonucleotide ProbesPhosphoproteinsRatsRats, Inbred StrainsReceptors, SteroidRecombinant ProteinsSequence Homology, Nucleic AcidTranscription FactorsTranscription, GeneticConceptsSteroid hormone receptor superfamilyHNF-4 proteinHNF-4Hormone receptor superfamilyReceptor superfamilyTranscription factor HNF-4Regulation of transcriptionAmino acid sequencePyruvate kinase geneLigand-dependent transcription factorSequence-specific fashionDNA binding activityPolymerase chain reactionHNF-4 mRNANorthern blot analysisUnusual amino acidsLF-A1CDNA clonesActivate transcriptionBinding to sitesZinc fingerCotransfection assaysDNA bindingSynthetic oligonucleotidesTranscription factors