In vitro assembly of multiprotein complexes containing alpha, beta, and gamma tubulin, heat shock protein HSP70, and elongation factor 1 alpha.
Marchesi V, Ngo N. In vitro assembly of multiprotein complexes containing alpha, beta, and gamma tubulin, heat shock protein HSP70, and elongation factor 1 alpha. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 3028-3032. PMID: 8464918, PMCID: PMC46230, DOI: 10.1073/pnas.90.7.3028.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCentrifugation, Density GradientCHO CellsCricetinaeElectrophoresis, Polyacrylamide GelHeat-Shock ProteinsHeLa CellsHumansImmunoblottingMacromolecular SubstancesMitosisModels, StructuralMolecular WeightNocodazolePeptide Elongation Factor 1Peptide Elongation FactorsRibonucleoproteinsTubulinConceptsElongation factor 1 alphaHeat shock protein Hsp70Multiprotein complexesShock protein Hsp70Factor 1 alphaGamma-tubulinProtein Hsp70Regulation of mitosisPresence of ATPDistinct complexesMitotic centrosomesActin isoformsSucrose gradient ultracentrifugationVitro assemblyTubulin isoformsLow abundanceCHO cellsHSP70Degrees C incubationProteinCognate formIsoformsSmall precursorsHigh-speed centrifugationComplexes