2015
Systemic Administration of the TRPV3 Ion Channel Agonist Carvacrol Induces Hypothermia in Conscious Rodents
Feketa V, Marrelli S. Systemic Administration of the TRPV3 Ion Channel Agonist Carvacrol Induces Hypothermia in Conscious Rodents. PLOS ONE 2015, 10: e0141994. PMID: 26528923, PMCID: PMC4631363, DOI: 10.1371/journal.pone.0141994.Peer-Reviewed Original ResearchConceptsHypothermic effectConscious patientsNovel pharmacological methodHigh intravenous dosesInduction of hypothermiaTherapeutic hypothermiaBlood pressureIntravenous dosesSuboptimal effectivenessConscious rodentsPromising new strategySystemic administrationIntravenous administrationCold-seeking behaviorPharmacological methodsSubstantial toxicityHypothermia inductionSafe dosesHypothermiaChannel activationPatientsCore temperatureMiceAdministrationDoses
2014
Transient Receptor Potential Melastatin 8 Channel Inhibition Potentiates the Hypothermic Response to Transient Receptor Potential Vanilloid 1 Activation in the Conscious Mouse
Feketa V, Zhang Y, Cao Z, Balasubramanian A, Flores C, Player M, Marrelli S. Transient Receptor Potential Melastatin 8 Channel Inhibition Potentiates the Hypothermic Response to Transient Receptor Potential Vanilloid 1 Activation in the Conscious Mouse. Critical Care Medicine 2014, 42: e355-e363. PMID: 24595220, PMCID: PMC4137592, DOI: 10.1097/ccm.0000000000000229.Peer-Reviewed Original ResearchConceptsTRP melastatin 8TRP vanilloid 1Transient receptor potential channelsConscious miceTRPM8 inhibitorTransient receptor potential vanilloid 1 (TRPV1) activationCore temperatureAged male miceProspective animal studyAcademic medical centerPotential channelsCerebral ischemiaLoading doseDose-dependent dropMechanical ventilationVanilloid 1TRPV1 agonistsCardiac arrestCold defense responsesContinuous infusionAged miceHypothermic effectTherapeutic rangeMale miceHypothermic response
2013
Shivering and tachycardic responses to external cooling in mice are substantially suppressed by TRPV1 activation but not by TRPM8 inhibition
Feketa V, Balasubramanian A, Flores C, Player M, Marrelli S. Shivering and tachycardic responses to external cooling in mice are substantially suppressed by TRPV1 activation but not by TRPM8 inhibition. AJP Regulatory Integrative And Comparative Physiology 2013, 305: r1040-r1050. PMID: 24005250, PMCID: PMC3840321, DOI: 10.1152/ajpregu.00296.2013.Peer-Reviewed Original ResearchConceptsTachycardic responsesTRPV1 activationMuscle activityCore temperatureTRPM8 inhibitionConscious stroke patientsRole of TRPM8TRPM8 KO miceTreatment of strokeElectromyographic muscle activityInduction of hypothermiaCold-induced shiveringTherapeutic hypothermiaStroke patientsConscious miceTRPV1 agonistsKO miceAbdominal cavityTachycardiaBack musclesEffective hypothermiaMild decreaseProfound dropShiveringHypothermiaAttenuation of cold‐induced shivering and tachycardia with modulators of thermosensitive TRPV1 and TRPM8 channels
Feketa V, Marrelli S. Attenuation of cold‐induced shivering and tachycardia with modulators of thermosensitive TRPV1 and TRPM8 channels. The FASEB Journal 2013, 27: 1201.27-1201.27. DOI: 10.1096/fasebj.27.1_supplement.1201.27.Peer-Reviewed Original ResearchCold-induced shiveringTRPV1 activationAfferent neural pathwaysCold-sensitive neuronsTRPM8 inhibitionCore temperatureTachycardic responsesTherapeutic hypothermiaAfferent pathwaysTRPM8 antagonistsCapsaicin receptorTRPM8 ion channelHeart rateMouse modelPharmacological modulationTachycardiaSkin warmingNeural pathwaysCombined treatmentShiveringTRPV1TRPM8 channelsIon channelsInhibitionComplete inhibition