Edges of human embryonic stem cell colonies display distinct mechanical properties and differentiation potential
Rosowski KA, Mertz AF, Norcross S, Dufresne ER, Horsley V. Edges of human embryonic stem cell colonies display distinct mechanical properties and differentiation potential. Scientific Reports 2015, 5: 14218. PMID: 26391588, PMCID: PMC4585749, DOI: 10.1038/srep14218.Peer-Reviewed Original ResearchMeSH KeywordsCell DifferentiationColony-Forming Units AssayCytoskeletonHuman Embryonic Stem CellsHumansMechanical PhenomenaConceptsHuman embryonic stem cellsUndifferentiated coloniesDifferentiation processCell fate decisionsHuman embryonic stem cell coloniesEmbryonic stem cell coloniesEmbryonic stem cellsEdges of coloniesStrong traction forcesStem cell coloniesEarly human developmentDevelopmental decisionsFate decisionsActin organizationLive imagingColony geometryMyosin activityCellular environmentSmall coloniesAdherent coloniesDifferentiation potentialCell coloniesDifferentiation efficiencyStem cellsColonies