2022
Predominance of Severe Plasma Leakage in Pediatric Patients With Severe Dengue in Puerto Rico
Paz-Bailey G, Sánchez-González L, Torres-Velasquez B, Jones E, Perez-Padilla J, Sharp T, Lorenzi O, Delorey M, Munoz-Jordan J, Tomashek K, Waterman S, Alvarado L, Rivera-Amill V. Predominance of Severe Plasma Leakage in Pediatric Patients With Severe Dengue in Puerto Rico. The Journal Of Infectious Diseases 2022, 226: 1949-1958. PMID: 35510941, PMCID: PMC10015274, DOI: 10.1093/infdis/jiac165.Peer-Reviewed Original ResearchConceptsSevere plasma leakageSevere dengueReverse transcriptase-polymerase chain reactionPlasma leakageSevere bleedingDengue virusHistory of feverDays of presentationTranscriptase-polymerase chain reactionYear oldsNonsevere dengueOrgan involvementCommon presentationPediatric patientsClinical manifestationsDengue patientsEmergency departmentLaboratory findingsIgM enzymePatientsSerum samplesImmunosorbent assayDengueChain reactionBleeding
2021
Early Release - Novel Assay to Measure Seroprevalence of Zika Virus in the Philippines - Volume 27, Number 12—December 2021 - Emerging Infectious Diseases journal - CDC
Adams C, Jadi R, Segovia-Chumbez B, Daag J, Ylade M, Medina F, Sharp T, Munoz-Jordan J, Yoon I, Deen J, Lopez A, de Silva A, Premkumar L. Early Release - Novel Assay to Measure Seroprevalence of Zika Virus in the Philippines - Volume 27, Number 12—December 2021 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2021, 27: 3073-3081. PMID: 34808091, PMCID: PMC8632176, DOI: 10.3201/eid2712.211150.Peer-Reviewed Original ResearchConceptsZIKV seroprevalenceDENV transmissionEnvelope domain IIIZika virusSerologic assaysInfectious Diseases journal - CDCType-specific antibodiesDengue virus serotypesYears of agePublic health laboratoriesRelated flavivirusesHealth laboratoriesFlaviviridae familySeroprevalenceChildren 9Virus serotypesDomain IIIVirusAntibodiesAssaysDENVFlavivirusesKnowledge gaps in the epidemiology of severe dengue impede vaccine evaluation
Sharp T, Anderson K, Katzelnick L, Clapham H, Johansson M, Morrison A, Harris E, Paz-Bailey G, Waterman S. Knowledge gaps in the epidemiology of severe dengue impede vaccine evaluation. The Lancet Infectious Diseases 2021, 22: e42-e51. PMID: 34265259, PMCID: PMC11379041, DOI: 10.1016/s1473-3099(20)30871-9.Peer-Reviewed Original ResearchConceptsDengue virus infectionSevere dengueVirus infectionDisease severityAnti-dengue virus antibodiesMajor organ failureHospital admissionOrgan failureVaccine evaluationClinical spectrumVaccine trialsPrevious infectionRisk factorsVirus antibodiesClinical studiesClinical practiceDengue virusStudy participantsInfectionLack of specificityHost factorsVirus characteristicsDengueEpidemiologyRange of agesFatal Dengue Acquired in Florida
Sharp T, Morris S, Morrison A, de Lima Corvino D, Santiago G, Shieh W, Rico E, Kopp E, Muñoz-Jordán J, Marttos A, Paz-Bailey G, Abbo L, Stanek D. Fatal Dengue Acquired in Florida. New England Journal Of Medicine 2021, 384: 2257-2259. PMID: 34107186, PMCID: PMC11304554, DOI: 10.1056/nejmc2023298.Peer-Reviewed Original ResearchDengue Virus Infections among Peace Corps Volunteers in Timor-Leste, 2018–2019
Sánchez-González L, Venuto M, Poe S, Major C, Baskara L, Abdiyeva S, Murphy D, Munoz-Jordan J, Medina F, Paz-Bailey G, Petersen K, Becker K, Sharp T. Dengue Virus Infections among Peace Corps Volunteers in Timor-Leste, 2018–2019. American Journal Of Tropical Medicine And Hygiene 2021, 104: 2202-2209. PMID: 33901000, PMCID: PMC8176509, DOI: 10.4269/ajtmh.21-0020.Peer-Reviewed Original ResearchConceptsDengue virus infectionMosquito avoidance behaviorsVirus infectionPeace Corps volunteersAnti-DENV IgM antibodiesPeace Corps Medical OfficersOngoing health risksReporting feverDENV infectionIgM antibodiesRecent infectionEpidemiological characteristicsClinical managementMedical recordsMosquito bitesBed netsCollected bloodHealth servicesTravel historyDisease severityDisease controlInfectionMedical officersPreventive measuresDengueEstimating incidence of infection from diverse data sources: Zika virus in Puerto Rico, 2016
Quandelacy T, Healy J, Greening B, Rodriguez D, Chung K, Kuehnert M, Biggerstaff B, Dirlikov E, Mier-y-Teran-Romero L, Sharp T, Waterman S, Johansson M. Estimating incidence of infection from diverse data sources: Zika virus in Puerto Rico, 2016. PLOS Computational Biology 2021, 17: e1008812. PMID: 33784311, PMCID: PMC8034731, DOI: 10.1371/journal.pcbi.1008812.Peer-Reviewed Original ResearchConceptsGuillain-Barré syndrome casesBlood bank dataIncidence of infectionZika virus epidemicSubset of casesSyndrome casesWeekly infectionsUnderlying incidenceZika virusArboviral casesInfectionSurveillance dataEpidemic preparednessZika epidemicVirus epidemicEpidemicIncidenceLarge proportionCasesWeeks
2020
Notes from the Field: Interpretation of Rapid Diagnostic Tests for Leptospirosis During a Dengue Outbreak — Yap State, Federated States of Micronesia, 2019
Dawson P, Marfel M, Galloway R, Tareg A, Paz-Bailey G, Muñoz-Jordán J, Sharp T, Adams L, Bower W. Notes from the Field: Interpretation of Rapid Diagnostic Tests for Leptospirosis During a Dengue Outbreak — Yap State, Federated States of Micronesia, 2019. MMWR Morbidity And Mortality Weekly Report 2020, 69: 1832-1833. PMID: 33270615, PMCID: PMC7714033, DOI: 10.15585/mmwr.mm6948a6.Peer-Reviewed Original ResearchEpidemiologic and spatiotemporal trends of Zika Virus disease during the 2016 epidemic in Puerto Rico
Sharp T, Quandelacy T, Adams L, Aponte J, Lozier M, Ryff K, Flores M, Rivera A, Santiago G, Muñoz-Jordán J, Alvarado L, Rivera-Amill V, Garcia-Negrón M, Waterman S, Paz-Bailey G, Johansson M, Rivera-Garcia B. Epidemiologic and spatiotemporal trends of Zika Virus disease during the 2016 epidemic in Puerto Rico. PLOS Neglected Tropical Diseases 2020, 14: e0008532. PMID: 32956416, PMCID: PMC7529257, DOI: 10.1371/journal.pntd.0008532.Peer-Reviewed Original ResearchConceptsZIKV diseaseDisease casesZika virusZIKV infectionArboviral diseasesEvidence of infectionZika virus diseaseLaboratory-based surveillancePuerto Rico DepartmentPublic health authoritiesIgM ELISAZIKV epidemicHigh incidenceChikungunya virusLaboratory evidenceHealth authoritiesDiseaseEarly detectionRT-PCRInfectionSocioeconomic factorsVirus diseaseEpidemicEarlier midpointIncidenceClinical Characteristics, Histopathology, and Tissue Immunolocalization of Chikungunya Virus Antigen in Fatal Cases
Sharp T, Keating M, Shieh W, Bhatnagar J, Bollweg B, Levine R, Blau D, Torres J, Rivera A, Perez-Padilla J, Munoz-Jordan J, Sanabria D, Fischer M, Garcia B, Tomashek K, Zaki S. Clinical Characteristics, Histopathology, and Tissue Immunolocalization of Chikungunya Virus Antigen in Fatal Cases. Clinical Infectious Diseases 2020, 73: e345-e354. PMID: 32615591, PMCID: PMC11307670, DOI: 10.1093/cid/ciaa837.Peer-Reviewed Original ResearchConceptsSeptic shockFatal casesTissue specimensMononuclear cellsSplenic follicular dendritic cellsChikungunya virus antigenIntra-alveolar hemorrhageFatal septic shockFollicular dendritic cellsChikungunya virus infectionCommon histopathologic findingsBlood mononuclear cellsReverse transcriptase-polymerase chain reactionTranscriptase-polymerase chain reactionCHIKV antigenSevere comorbiditiesAcute illnessClinical characteristicsCHIKV infectionDendritic cellsMedian ageRecord reviewIllness onsetImmunohistochemical findingsHistopathologic findingsLaboratory-Acquired Dengue Virus Infection, United States, 2018 - Volume 26, Number 7—July 2020 - Emerging Infectious Diseases journal - CDC
Sharp T, Fisher T, Long K, Coulson G, Medina F, Herzig C, Koza M, Muñoz-Jordán J, Paz-Bailey G, Moore Z, Williams C. Laboratory-Acquired Dengue Virus Infection, United States, 2018 - Volume 26, Number 7—July 2020 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2020, 26: 1534-1537. PMID: 32568040, PMCID: PMC7323529, DOI: 10.3201/eid2607.191598.Peer-Reviewed Original ResearchReverse Transcription-Polymerase Chain Reaction Testing on Filter Paper-Dried Serum for Laboratory-Based Dengue Surveillance-American Samoa, 2018.
Curren E, Tufa A, Hancock W, Biggerstaff B, Vaifanua-Leo J, Montalbo C, Sharp T, Fischer M, Hills S, Gould C. Reverse Transcription-Polymerase Chain Reaction Testing on Filter Paper-Dried Serum for Laboratory-Based Dengue Surveillance-American Samoa, 2018. American Journal Of Tropical Medicine And Hygiene 2020, 102: 622-624. PMID: 31933466, PMCID: PMC7056409, DOI: 10.4269/ajtmh.19-0800.Peer-Reviewed Original ResearchConceptsReal-time reverse transcription-polymerase chain reactionResource-limited settingsTranscription polymerase chain reaction testingArboviral diseasesReverse transcription-polymerase chain reactionLaboratory-based surveillanceTranscription-polymerase chain reactionDengue virus RNADENV RNAReaction testingDiagnostic testingDengue surveillanceReference laboratorySerumSurveillance of dengueVirus RNAChain reactionDiseaseSurveillanceDengueTravel-Associated and Locally Acquired Dengue Cases — United States, 2010–2017
Rivera A, Adams L, Sharp T, Lehman J, Waterman S, Paz-Bailey G. Travel-Associated and Locally Acquired Dengue Cases — United States, 2010–2017. MMWR Morbidity And Mortality Weekly Report 2020, 69: 149-154. PMID: 32053577, PMCID: PMC7017959, DOI: 10.15585/mmwr.mm6906a1.Peer-Reviewed Original ResearchConceptsReverse transcription-polymerase chain reactionDengue casesDENV nonstructural protein 1 (NS1) antigenNonstructural protein 1 antigenMosquito-transmitted dengue virusAcute febrile illnessIgM positive casesAnti-DENV antibodiesTranscription-polymerase chain reactionProbable dengue casesCompetent mosquito vectorsFebrile illnessMedian ageDENV infectionFatal casesDENV antigenEpidemiologic trendsTravel-associatedDengue virusFamily FlaviviridaeVirus RNADistrict of ColumbiaChain reactionMosquito vectorsAntigen
2017
Differences in Prevalence of Symptomatic Zika Virus Infection, by Age and Sex—Puerto Rico, 2016
Lozier MJ, Burke RM, Lopez J, Acevedo V, Amador M, Read JS, Jara A, Waterman SH, Barrera R, Muñoz-Jordan J, Rivera-Garcia B, Sharp TM. Differences in Prevalence of Symptomatic Zika Virus Infection, by Age and Sex—Puerto Rico, 2016. The Journal Of Infectious Diseases 2017, 217: 1678-1689. PMID: 29216376, PMCID: PMC8849542, DOI: 10.1093/infdis/jix630.Peer-Reviewed Original ResearchConceptsZIKV infectionReverse transcription-polymerase chain reactionEnzyme-linked immunosorbent assaySex-dependent differencesSymptomatic infectionFemale sexSymptomatic Zika virus infectionZika virus infectionSymptomatic ZIKV infectionZika virus diseaseTranscription-polymerase chain reactionNonpregnant womenResidents of householdsZIKV diseaseRecent illnessVirus infectionDisease casesDiagnostic testingStudy participantsInfectionImmunosorbent assayConvenience samplePotential mechanismsChain reactionAgeAcute Zika Virus Infection as a Risk Factor for Guillain-Barré Syndrome in Puerto Rico
Dirlikov E, Medina NA, Major CG, Munoz-Jordan JL, Luciano CA, Rivera-Garcia B, Sharp TM. Acute Zika Virus Infection as a Risk Factor for Guillain-Barré Syndrome in Puerto Rico. JAMA 2017, 318: 1498-1500. PMID: 29049645, PMCID: PMC5817969, DOI: 10.1001/jama.2017.11483.Peer-Reviewed Original ResearchZika Virus -10 Public Health Achievements in 2016 and Future Priorities.
Oussayef N, Pillai S, Honein M, Ben Beard C, Bell B, Boyle C, Eisen L, Kohl K, Kuehnert M, Lathrop E, Martin S, Martin R, McAllister J, McClune E, Mead P, Meaney-Delman D, Petersen B, Petersen L, Polen K, Powers A, Redd S, Sejvar J, Sharp T, Villanueva J, Jamieson D. Zika Virus -10 Public Health Achievements in 2016 and Future Priorities. MMWR Morbidity And Mortality Weekly Report 2017, 65: 1482-1488. PMID: 28056005, DOI: 10.15585/mmwr.mm6552e1.Peer-Reviewed Original ResearchConceptsZika virusPregnant womenZika virus infectionEffective reversible contraceptionPersonal protective practicesPublic health achievementsPublic health activitiesPublic health agenciesPublic health emergencyWorld Health OrganizationSexual transmissionUnintended pregnancyReversible contraceptionVirus infectionCongenital microcephalyEmergency Operations CenterHealth achievementsHealth activitiesHealth agenciesHealth OrganizationHealth emergencyPregnancyVirusProtective practicesWomen
2016
Use of Household Cluster Investigations to Identify Factors Associated with Chikungunya Virus Infection and Frequency of Case Reporting in Puerto Rico
Bloch D, Roth NM, Caraballo EV, Muñoz-Jordan J, Hunsperger E, Rivera A, Pérez-Padilla J, Garcia B, Sharp TM. Use of Household Cluster Investigations to Identify Factors Associated with Chikungunya Virus Infection and Frequency of Case Reporting in Puerto Rico. PLOS Neglected Tropical Diseases 2016, 10: e0005075. PMID: 27764085, PMCID: PMC5072658, DOI: 10.1371/journal.pntd.0005075.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseAdolescentAdultAgedAged, 80 and overAir ConditioningAntibodies, ViralChikungunya FeverChikungunya virusChildChild, PreschoolCymbopogonDisease NotificationDisease OutbreaksEnzyme-Linked Immunosorbent AssayExanthemaFamily CharacteristicsFemaleFeverHumansInfantMaleMiddle AgedPoint-of-Care SystemsPolymerase Chain ReactionPuerto RicoYoung AdultConceptsSymptomatic CHIKV infectionRecent CHIKV infectionCHIKV infectionPublic health authoritiesChikungunya virusChikungunya patientsMedical careHealth authoritiesCase reportingAnti-CHIKV IgMAcute febrile illnessChikungunya virus infectionCare rapid diagnostic testsRapid diagnostic testsPuerto Rico DepartmentCluster investigationsAcute illnessFebrile illnessSkin rashResidents of householdsDevelopment of diseaseMultivariable analysisIllness onsetIgG antibodiesIgM ELISAEarly Indicators of Fatal Leptospirosis during the 2010 Epidemic in Puerto Rico
Sharp TM, García B, Pérez-Padilla J, Galloway RL, Guerra M, Ryff KR, Haberling D, Ramakrishnan S, Shadomy S, Blau D, Tomashek KM, Bower WA. Early Indicators of Fatal Leptospirosis during the 2010 Epidemic in Puerto Rico. PLOS Neglected Tropical Diseases 2016, 10: e0004482. PMID: 26914210, PMCID: PMC4767218, DOI: 10.1371/journal.pntd.0004482.Peer-Reviewed Original ResearchConceptsFirst medical visitWhite blood cellsLeptospirosis patientsFatal outcomeMedical visitsFatal leptospirosisLaboratory valuesHospitalized leptospirosis patientsNon-fatal patientsRoutine laboratory valuesLeg painControl patientsMost patientsCase patientsHospital admissionMultivariable analysisIllness onsetPlatelet countDengue patientsFatal casesWBC countLeptospirosis epidemicPatientsMedical careOld male
2013
Dengue outbreak--Federated States of Micronesia, 2012-2013.
Taulung L, Masao C, Palik H, Samo M, Barrow L, Pretrick M, Nilles E, Kool J, Aure F, Simmons R, Moore F, Pyke A, Taylor C, Cao-Lormeau V, Sharp T, Chen T. Dengue outbreak--Federated States of Micronesia, 2012-2013. MMWR Morbidity And Mortality Weekly Report 2013, 62: 570-3. PMID: 23863704, PMCID: PMC4604812.Peer-Reviewed Original ResearchConceptsRapid diagnostic testsNonstructural protein 1World Health OrganizationHealth servicesDengue virus nonstructural protein 1Dengue outbreakHospital surge capacityVirus nonstructural protein 1Resource-limited settingsPublic health activitiesPublic health servicesOutbreak response plansMuscle painClinical managementImmunoglobulin MEnhanced surveillanceEpidemiologic surveillanceSurge capacityHealth consequencesDiagnostic testsHealth activitiesHealth OrganizationProtein 1Laboratory servicesDengueVirus-Specific Differences in Rates of Disease during the 2010 Dengue Epidemic in Puerto Rico
Sharp TM, Hunsperger E, Santiago GA, Muñoz-Jordan JL, Santiago LM, Rivera A, Rodríguez-Acosta RL, Feliciano L, Margolis HS, Tomashek KM. Virus-Specific Differences in Rates of Disease during the 2010 Dengue Epidemic in Puerto Rico. PLOS Neglected Tropical Diseases 2013, 7: e2159. PMID: 23593526, PMCID: PMC3617145, DOI: 10.1371/journal.pntd.0002159.Peer-Reviewed Original ResearchConceptsAcute febrile illnessReverse transcriptase-polymerase chain reactionLaboratory-positive casesPrimary infectionDengue casesDENV-1DENV-2Anti-DENV IgM antibodiesSerum specimenMortality of dengueMosquito-transmitted dengue virusPrimary DENV infectionPassive dengue surveillance systemHealth care providersPrimary prevention interventionsTranscriptase-polymerase chain reactionOnly age groupDENV nucleic acidLaboratory diagnostic dataRates of diseaseFebrile illnessSevere dengueDENV infectionIgM antibodiesDENV antigen