2020
Reverse Transcription-Polymerase Chain Reaction Testing on Filter Paper-Dried Serum for Laboratory-Based Dengue Surveillance-American Samoa, 2018.
Curren E, Tufa A, Hancock W, Biggerstaff B, Vaifanua-Leo J, Montalbo C, Sharp T, Fischer M, Hills S, Gould C. Reverse Transcription-Polymerase Chain Reaction Testing on Filter Paper-Dried Serum for Laboratory-Based Dengue Surveillance-American Samoa, 2018. American Journal Of Tropical Medicine And Hygiene 2020, 102: 622-624. PMID: 31933466, PMCID: PMC7056409, DOI: 10.4269/ajtmh.19-0800.Peer-Reviewed Original ResearchConceptsReal-time reverse transcription-polymerase chain reactionResource-limited settingsTranscription polymerase chain reaction testingArboviral diseasesReverse transcription-polymerase chain reactionLaboratory-based surveillanceTranscription-polymerase chain reactionDengue virus RNADENV RNAReaction testingDiagnostic testingDengue surveillanceReference laboratorySerumSurveillance of dengueVirus RNAChain reactionDiseaseSurveillanceDengue
2017
Risk Factors Associated with Persistence of Zika Virus Nucleic Acid in Serum and Semen
Lozier M, Rosenberg E, Doyle K, Adams L, Klein L, Muñoz-Jordan J, Alvarado L, Sharp T, Paz-Bailey G. Risk Factors Associated with Persistence of Zika Virus Nucleic Acid in Serum and Semen. Open Forum Infectious Diseases 2017, 4: s56-s57. PMCID: PMC5632286, DOI: 10.1093/ofid/ofx162.133.Peer-Reviewed Original ResearchDetectable ZIKV RNADays post-symptom onsetLonger median timeNon-pregnant womenZIKV RNAPregnant womenSymptom onsetMedian timeStudy participationRT-PCRPost-symptom onsetDuration of detectionRT-PCR testingZika virus RNAProportion of menVirus nucleic acidZika virus nucleic acidProspective cohortPrevention recommendationsRisk factorsIndex caseDiagnostic testingFirst monthConjunctivitisSerumPersistence of Zika Virus in Body Fluids — Final Report
Paz-Bailey G, Rosenberg ES, Doyle K, Munoz-Jordan J, Santiago GA, Klein L, Perez-Padilla J, Medina FA, Waterman SH, Adams L, Lozier M, Bertrán-Pasarell J, Garcia Gubern C, Alvarado L, Sharp T. Persistence of Zika Virus in Body Fluids — Final Report. New England Journal Of Medicine 2017, 379: 1234-1243. PMID: 28195756, PMCID: PMC5831142, DOI: 10.1056/nejmoa1613108.Peer-Reviewed Original ResearchZIKV RNAZIKV RNA detectionVaginal secretionsIgM enzyme-linked immunosorbentDetectable ZIKV RNARNA detectionZika virus RNAEnzyme-linked immunosorbentZIKV exposureZIKV infectionWeek 4RNA clearanceZika virusSurveillance sitesFirst monthRT-PCRSerumVirus RNAMonthsBody fluidsUrineSecretionDaysMedianRegression models