2013
Proximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion
Li H, Du Z, Barone S, Rubera I, McDonough A, Tauc M, Zahedi K, Wang T, Soleimani M. Proximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion. Journal Of Molecular Medicine 2013, 91: 951-963. PMID: 23508938, PMCID: PMC3730089, DOI: 10.1007/s00109-013-1015-3.Peer-Reviewed Original ResearchConceptsNa+/H+ exchanger NHE3Proximal convoluted tubulesKO miceMetabolic acidosisBicarbonate reabsorptionDays of acid loadingIsolated proximal convoluted tubulesConvoluted tubulesIn vitro microperfusion studyCompared to WT miceNHE3 knockout miceProximal tubule NHE3Acid loadNH4Cl acid loadMild metabolic acidosisWT miceNHE3Microperfusion studiesKnockout miceBicarbonate absorptionMutant miceVolume reabsorptionFloxed miceNH4Cl loadingTransgenic mice
2003
Intracellular Na concentration and Rb uptake in proximal convoluted tubule cells and abundance of Na/K-ATPase α1-subunit in NHE3−/− mice
Beck F, Neuhofer W, Dörge A, Giebisch G, Wang T. Intracellular Na concentration and Rb uptake in proximal convoluted tubule cells and abundance of Na/K-ATPase α1-subunit in NHE3−/− mice. Pflügers Archiv - European Journal Of Physiology 2003, 446: 100-105. PMID: 12690468, DOI: 10.1007/s00424-002-1001-z.Peer-Reviewed Original ResearchConceptsNhe3-/- miceCell Na concentrationIntracellular Na concentrationProximal convoluted tubulesNhe3-/-Proximal convoluted tubule cellsNa/K-ATPaseNa/H exchanger isoform 3Rb uptakeConvoluted tubulesExpression of Na/K-ATPaseApical Na entryExchanger isoform 3Intracellular Cl- concentrationNa/K-ATPase activityDistal convoluted tubuleConvoluted tubule cellsApical NHE3NHE3 deficiencyNa entryIntercalated cellsWestern blot analysisNa concentrationTubule cellsRenal cortex
1991
The role of phosphoinositide turnover in mediating the biphasic effect of angiotensin II on renal tubular transport.
Wang T, Chan Y. The role of phosphoinositide turnover in mediating the biphasic effect of angiotensin II on renal tubular transport. Journal Of Pharmacology And Experimental Therapeutics 1991, 256: 309-17. PMID: 1846421.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAngiotensin IIAnimalsBicarbonatesBiological TransportCalcium Channel BlockersDose-Response Relationship, DrugGallic AcidIsoquinolinesKidney Tubules, ProximalMalePerfusionPhosphatidylinositolsPiperazinesProtein Kinase InhibitorsRatsRats, Inbred StrainsSignal TransductionSodiumTetradecanoylphorbol AcetateConceptsEffects of Ang IIProximal convoluted tubulesAng IITMB-8Biphasic effectAngiotensin IISodium transportStimulatory effect of Ang IIPhosphoinositide turnoverN-diethylamino)-octyl-3,4,5-trimethoxybenzoateDose-dependent biphasic effectEffects of angiotensin IIRat proximal convoluted tubuleIntracellular calcium mobilizationRenal tubular transportOriginal perfusatePerfusion in vivoIntracellular calciumNa+/H+ exchangeCalcium mobilizationJHCO3Fluid reabsorptionConvoluted tubulesLuminal perfusionRat kidney
1990
Time- and dose-dependent effects of protein kinase C on proximal bicarbonate transport
Wang T, Chan Y. Time- and dose-dependent effects of protein kinase C on proximal bicarbonate transport. The Journal Of Membrane Biology 1990, 117: 131-139. PMID: 2120446, DOI: 10.1007/bf01868680.Peer-Reviewed Original ResearchConceptsProximal convoluted tubulesProtein kinase CKinase CFluid reabsorptionPCT transportRate of bicarbonate absorptionEffects of protein kinase CActivation of endogenous protein kinase CEndogenous protein kinase CActivation of protein kinase CBicarbonate transport activityInhibition of transport processesDose-dependent effectActivate protein kinase COriginal perfusateBasolateral sideDose-response natureBicarbonate absorptionBrush-border membranePerfusion timeMaximal effectConvoluted tubulesLuminal perfusionRat kidneyRenal tubulesCholinergic effect on rat proximal convoluted tubule
Wang T, Chan Y. Cholinergic effect on rat proximal convoluted tubule. Pflügers Archiv - European Journal Of Physiology 1990, 415: 533-539. PMID: 2109302, DOI: 10.1007/bf02583503.Peer-Reviewed Original ResearchConceptsPCT transportProximal convoluted tubulesA-23187Cholinergic receptorsHigher doses of carbacholInhibitory effect of carbacholRate of bicarbonate absorptionConvoluted tubulesCholinergic effectsRegulation of renal functionRat proximal convoluted tubuleEffects of cholinergic agentsEffect of carbacholAddition of carbacholDose of carbacholFunctional cholinergic receptorsCarbachol-induced effectsProximal tubular absorptionIonophore A-23187Bicarbonate-Ringer solutionInhibitory effectNo effectRenal functionBasolateral sideCalcium influx